Analysis Of Organoleptic And Coliform Value In Fresh Mackerel (Rastrelliger Sp.) Fish In Tpi Sorong City

Sukmawati, Sukmawati; Badaruddin, Iksan; Dewi, Nurul Kusuma; Situmorang, Nurbaity; Mahfut, Mahfut; Mustapa, Fery

doi:10.1088/1742-6596/1764/1/012035

Cited by 3 publications

(2 citation statements)

References 6 publications

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“…Kontaminasi mikroba dapat diketahui melalui uji total plate count (TPC), uji coliform, uji staphylococcus, dan uji Salmonella (Sukmawati, et. al., 2021).…”

Section: Pendahuluanunclassified

Pengaruh Lama Penyimpanan Produk Ikan Cakalang (Katsuwonus pelamis) Asap Terhadap Nilai Mutu Organoleptik Dan Nilai Mutu Mikrobiologi Di Pasar Remu Kota Sorong

Sukmawati

2021

JB&P

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Proses pengasapan ikan di Indonesia khususnya di Sorong, masih dilakukan pengasapan secara tradisionall dengan modal dan skala usaha kecil sehingga penggunaan alat masih sederhana, selain itu sanitasi dan hygiene masih kurang diperhatikan dalam penanganan dan pengolahannya. Produk ikan yang diproses secara tradisional sangat rentan terhadap kerusakan mikrobiologi akibat kontaminasi bakteri patogen, jamur patogen maupun racun yang dihasilkan. Tujuan dari penelitian ini ialah untuk mengetahui pengaruh lama penyimpanan ikan cakalang (Katsuwonus pelamis) asap tehadap nilai mutu organoleptik dan nilai mutu Mikrobiologi di Pasar Remu Kota Sorong, dan untuk mengetahui jumlah koloni pada ikan cakalang (Katsuwonus pelamis) asap di Pasar Remu Kota Sorong. Metode yang dalam penalitian ini yakni deskriptif dan eksperimen. Perlakuan lama penyimpanan pada ikan cakalang (Katsuwonus pelamis) asap pada hari ke-1,hari ke-3,hari ke-5 memberikan pengaruh terhadap nilai mutu organoleptik untuk kenampakan ikan, tekstur ikan, bau ikan rasa ikan, dan terdapat jamur, sedangkan untuk pengamatan lendir tidak memberikan pengaruh pada ketiga sampel baik dari hari ke-1,hari ke-3, maupun hari ke-5 sampel tidak menunjukan adanya lendir pada bagian dalam maupun luar daging ikan. Jumlah total plate mikroba pada ikan cakalang asap pada hari ke-1 untuk sampel A = 1,6X105 ,sampel B =1,2X105 dan sampel C = 3,6X104 menunjukan bahwa total plate count(TPC) tidak melwati batas standar maksimum SNI, Sedangkan untuk pengamatan hari ke-3 dan hari ke-5 baik sampel A, sampel B, maupun sampel C jumlah total plate count mikroba pada sampel tersebut melewati batas maksimum SNI.

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“…Kontaminasi mikroba dapat diketahui melalui uji total plate count (TPC), uji coliform, uji staphylococcus, dan uji Salmonella (Sukmawati, et. al., 2021).…”

Section: Pendahuluanunclassified

Pengaruh Lama Penyimpanan Produk Ikan Cakalang (Katsuwonus pelamis) Asap Terhadap Nilai Mutu Organoleptik Dan Nilai Mutu Mikrobiologi Di Pasar Remu Kota Sorong

Sukmawati

2021

JB&P

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“…The OMF presence in plants can protect them from ORSV infection (Otero et al, 2013;Minh et al, 2016;Mahfut, 2021). Enhanced production of antagonistic enzymes and secondary metabolites can prevent and decrease the severity of disease caused by pathogenic infections (Sukmawati et al, 2021). Trichoderma sp.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Mycorrhiza and Its Potential as Biocontrol

MAHFUT

2023

SABRAOJBG

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Orchid mycorrhizal fungi (OMF) are vital biocontrol agents, especially for Odontoglossum ringspot virus (ORSV). The promising study helped identify the mycorrhiza isolate from native tropical orchids and determine its potential as a biocontrol. Sample collection of healthy roots of Phalaenopsis amabilis emanated in Yogyakarta, Indonesia, carrying out molecular identification with rDNA-ITS amplification using a set of universal primers ITS1 and ITS4. In vivo, antagonist tests began by inoculating viruses and mycorrhiza to determine the effect of growth and induction of secondary metabolites. The result showed one isolate of Trichoderma sp. associated with the molecular analysis has amplified the ITS1- 5.8S-ITS4 section by 600–750 bp DNA. The sequenced products revealed insertion and substitution occurrences, which may have caused the variance by strain diversity and potential severity. Indonesian isolates have undergone speciation and separation from other isolates by a substantial distance. The considerable effects were the increase in leaf length, leaf width, root length, leaf count, the number of roots, fresh weight, and a lowering of the virus content. The analysis of the plant growth parameters and virus concentrations provided significant differences among the treatments inoculated with orchid mycorrhiza (Mycorrhiza [M], Mycorrhiza + Virus [MV], and Virus + Mycorrhiza [VM]) and those without orchid mycorrhiza inoculation (Control [C] and Virus [V]). The orchid resistance suggested that the virus infecting plant leaves contain more phenolic chemicals. This study is the first-ever report of the Trichoderma sp. isolated from native tropical orchids in Indonesia.

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Screening of Sugarcane Commercial GMP Varieties Tolerant to Drought Stress Based on Molecular Detection of P5CS Gene

Mahfut,

Ananda,

Susiyanti

et al. 2024

J. Multidiscip. Appl. Nat. Sci.

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Sugarcane (Saccharum officinarum L.) requires sufficient a water supply to produce optimal productivity. The selection of the superior varieties of sugarcane that are tolerant to drought can be done by molecular detection of the presence of the pyrroline-5-carboxylate synthetase (P5CS) gene. The research is aimed at molecular detection of P5CS gene in a commercial varieties at PT Gunung Madu Plantations (GMP), Indonesia that are potentially drought resistant. Similar research has never been conducted at PT GMP, as evidenced by the absence of official publications. The varieties used are GMP 3, GMP 5, RGM 06-654, PS 864, RGM 08-1026, PSJT 941, RGM 01-1834, GP11, RGM 07-099, and RGM 02-108. The research phases include DNA isolation, quantitative and qualitative DNA amplification, and PCR product visualization. Molecular data analysis is based on DNA band scoring results in the form of binary data which is then used to calculate the level of polimorphism information content (PIC) on the primary data used. The results of the study showed that nine out of ten varieties had P5CS-specific band sizes of ±167 bp, namely GMP 3, GMP 5, RGM 06-654, PS 864, PSJT 941, RGM 01-1834, GP 11, RGM 07-099, and RGM 02-108. A total of four of the nine varieties showed P5CS gene band thicknesses of RGM06-654, PSJT 941, PS864, and RGM 01-1834 which indicates the selected varieties most resistant to drought. The result of the PIC calculation has a value of > 0.25 which indicates the primary P5CS used is quite informative. The molecular detection was continued on one selected variety is PSJT 941, which showed that the relationship analysis of P5CS gene shows close affinity with the isolates from China and Bogor.

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Analysis Of Organoleptic And Coliform Value In Fresh Mackerel (Rastrelliger Sp.) Fish In Tpi Sorong City

Cited by 3 publications

References 6 publications

Pengaruh Lama Penyimpanan Produk Ikan Cakalang (Katsuwonus pelamis) Asap Terhadap Nilai Mutu Organoleptik Dan Nilai Mutu Mikrobiologi Di Pasar Remu Kota Sorong

Pengaruh Lama Penyimpanan Produk Ikan Cakalang (Katsuwonus pelamis) Asap Terhadap Nilai Mutu Organoleptik Dan Nilai Mutu Mikrobiologi Di Pasar Remu Kota Sorong

Molecular Characterization of Mycorrhiza and Its Potential as Biocontrol

Screening of Sugarcane Commercial GMP Varieties Tolerant to Drought Stress Based on Molecular Detection of P5CS Gene

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