Analysis of parameters characterizing argyrophilic zones in intact lymphocytes of domestic sheep (&lt;i&gt;Ovis aries L.&lt;/i&gt;, 1758) and their hybrids with argali (&lt;i&gt;Ovis ammon L.&lt;/i&gt;, 1758)

Klenovitsky, P. M.; Иолчиев, Б. С.; Ветох, А. Н.

doi:10.32634/0869-8155-2021-344-1-52-56

Cited by 3 publications

(1 citation statement)

References 7 publications

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“…The same authors investigated NOR activity in metaphase chromosomes while studying the species diversity of animals. Russian scientists determined the differences between the groups of sheep of different genotypes by the number of NORs in interphase cells and the density of their staining (Klenovitsky PM et al, 2021). The authors believe that these indices characterize the state of argophilic zones of the cell nucleus.…”

Section: Discussionmentioning

confidence: 99%

Polymorphism of nucleolar organizer regions in different Ukrainian cattle breeds

2021

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Aim. To study the activity of nucleolar organizer regions (NORs) in different Ukrainian cattle breeds in terms of their apparent activity status in silver stain and possible relation with milk productivity. Methods. Chromosome prepara- tions using lymphocytes from the peripheral blood of 90 cows of different breeds were used in the study. NOR activity was determined by visual evaluation of concentrations of silver precipitation on NORs in individual chromosomes. A 50 % silver nitrate solution was used to stain chromosome preparations. NORs were detected as dark spots on telomeres of the corresponding chromosomes. Results. The cytological analysis of chromosome preparations from lymphocytes of first lactation cows detected NOR polymorphism in Ukrainian Red-and-Motley dairy cattle (URM), Ukrainian Black-and-White dairy breed (UBW), and hybrid cows, obtained by crossing Ukrainian Red-and-Motley dairy breed and Montbeliarde bulls (URM × M). First lactation cows of URM and UBM had higher or the same inci- dence of cells with four (29.8 and 30 %) and five (17.1 and 19.5 %) NORs, while in URM × M cows the incidence of cells with the same number of NORs was almost twice lower; cells with 7 and 8 NORs exceeded a similar index for other investigated breeds almost twice (2.5 against 4.5 % and 2.0 against 4.2 %). The highest level of chromosomal aberrations (CA) was observed in the group of animals with medium number (2 to 3 NORs per cell), and the lowest – in the group with a high number of NORs (from 6 to 7) with a reliable intergroup difference (p < 0.01). NOR activity was the highest in the group of animals of local origin (URM × M) with a milk yield over 7,000 kg in 305 days of the first lactation and the lowest in the UBW cows with a milk yield of 4–5,000 kg during the first lactation. Conclusions. We determined the differences in the activity of nucleolar organizers between the investigated groups of cows of dairy breed. URM × M hybrids reliably (р ≤ 0.05) exceeded dairy UBW cows by this index. No statistically significant different was found between other investigated groups of animals by this trait. Higher dairy productivity was found in the animals with higher frequency of NORs in the chromosomes of metaphase cells. In our opinion, the number of active NORs demonstrates relative variability between their number and the rate of protein synthesis, required to implement the productivity traits of the investigated animals.

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Section: Discussionmentioning

confidence: 99%

Polymorphism of nucleolar organizer regions in different Ukrainian cattle breeds

2021

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Investigation of some nucleoli traits in interphase leukocytes of two rabbit breeds and their hybrid

Dsitsiuk,

Boiko,

Honchar

et al. 2024

Agric. sci. pract.

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Aim. The aim was to study the differences in three traits of nucleoli in interphase leukocytes of two rab- bit breeds and a hybrid thereof. Methods. Doe rabbits, 4-month -old, from Ukrainian breed Poltavske Sriblo (n = 30), Californian (n = 25), and their hybrid (n = 21) were used in the experiment. The nucleoli in intact blood leukocytes were stained using silver staining according to Howell and Black (1980). Stained cells were observed, and their nucleoli counted in light microscopy at 10×100 oil immersion magnification. 200 leukocytes from each animal were analyzed. The activity of nucleoli was evaluated by the following parameters: the aver- age number of nucleoli in the nucleus (nNO), the total area of nucleoli in the nucleus (ΣSNO, mcm 2), the share of nucleolus area in the lymphocyte nucleus area (shΣS NO, %). Statistical analysis was conducted using the STATISTICA software package (2020). Results. The average number of nucleoli per cell varied from – 1.70 ± 0.08 in Californian rabbits to 5.90 ± 0.29 in hybrid animals. A statistically significant difference (p < 0.05) was found between the experimental groups of purebred and hybrid rabbits. The variation coefficient for the index of the average number of nucleoli per cell was on the average level of variability: 20.58 % for the rabbits of Poltavske sriblo breed, 19.50 % for Californian rabbits, and 16.49 % for hybrid ones. The total area of nucleoli in the cells of all the investigated animals varied from 5 μm2 in one Californian rabbit to 12 μm2 in animals of hybrid origin. The share of the nucleolus area in the nucleus area for rabbits of Poltavske sriblo, Californian, and hybrid breeds was 26.10 ± 1.80 %, 24.30 ± 1.62 and 29.40 ± 2.50, respectively. Conclusions. Polymorphism was observed for three nucleolar parameters after silver staining of interphase leukocytes of rabbits of Poltavske sriblo, Californian breed and their hybrid. This concerned 1) the average number of nucleoli (p < 0.05); 2) the total area of nucleoli (p < 0.05); 3) the average share of the nucleolar area in the nucleus area (p < 0.05). The results of our comparative analysis of the investigated nucleolar activity parameters suggest a higher activity of nucleoli in the animals of hybrid origin. In the future, the results of such studies may be used to assess the potential ability of animals to implement productive traits.

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Prospects for the use of modern methods of staining nucleolar organizers regions of cells for the diagnosis of diseases in animals

Novgorodova¹

2022

Agrarnaâ nauka

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Relevance. In recent years, issues of human and animal oncology have been considered in a broad aspect as a separate interdisciplinary science. Some diseases are more common in animals than in humans. That is why the selection of the necessary methods of purposeful value is required. In connection with the above data, the relevance of the work lies in the consideration of various methods of using NOR cells for the diagnosis of diseases of various etiologies in animals.Methods and results. In order to determine a particular disease, it is necessary not only to carefully study the available histopathological methods, but also to develop new ones. The comparability of human, bovine, sheep, and other mammalian gene maps opens up the possibility of applying methods used in medicine for animals (for example, FISH analysis with human DNA probes). Argyrophilic proteins associated with NOR ofcells are widely used in diagnostic pathology of a different nature. AgNOR staining method is considered as a proliferation marker. The use of the NOR method is promising in the differentiation of malignant and benign diseases. This method consists of counting the number of nuclei, determining the area and size of the nucleoli. Thus, methods based on the calculation of NOR are the most promising for diagnosing various pathologies in animals, including taking into account the prognosis of diseases.

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Analysis of parameters characterizing argyrophilic zones in intact lymphocytes of domestic sheep (<i>Ovis aries L.</i>, 1758) and their hybrids with argali (<i>Ovis ammon L.</i>, 1758)

Cited by 3 publications

References 7 publications

Polymorphism of nucleolar organizer regions in different Ukrainian cattle breeds

Polymorphism of nucleolar organizer regions in different Ukrainian cattle breeds

Investigation of some nucleoli traits in interphase leukocytes of two rabbit breeds and their hybrid

Prospects for the use of modern methods of staining nucleolar organizers regions of cells for the diagnosis of diseases in animals

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