Analysis of PCBs in waste oil by enzyme immunoassay

Lambert, Nadine M.; Fan, Titan S.; Pilette, J. F.

doi:10.1016/s0048-9697(96)05389-2

Cited by 18 publications

(7 citation statements)

References 3 publications

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“…The detection limits, defined as the detectable concentration equivalent to twice the standard deviation of zero binding, were within 5.0 to 12.9 µg L -1 , which is low enough for quantification of PCB in soil samples. PCB levels of 50 µg g -1 or more constitutes soil contamination [7,17]. Because 5 g soil was extracted with 5 mL organic solvent in this ELISA and the soil extract was diluted 100 times for ELISA, the detection limit achieved by use of this ELISA for soil analysis will be 0.5-1.29 µg g -1 soil, which is approximately 40-100 times lower than the value of 50 µg g -1 .…”

Section: Resultsmentioning

confidence: 99%

Direct competitive ELISA for the determination of polychlorinated biphenyls in soil samples

et al. 2002

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A direct competitive ELISA for determination of polychlorinated biphenyls (PCB) in soil samples was described. The standard calibration curve based on Delor 103 (Aroclor 1242) was constructed in the dynamic range of 10-1000 micro g L(-1) and a detection limit of 5.0-12.9 micro g L(-1) (or 0.5-1.29 micro g g(-1) soil) was achieved. When spiked soil samples were extracted with methanol recoveries were 90.6-106.3 %. The effect of methanol and DMSO on assay signal and sensitivity was established. Eight PCB-contaminated soil samples were analyzed by ELISA and gas chromatography (GC). The ELISA results from Soxhlet extraction were in a good agreement with those of GC (correlation coefficient 0.9866; n=8). Except for one soil sample the results from ELISA with methanol extraction were not significantly different from those from GC.

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Section: Resultsmentioning

confidence: 99%

Direct competitive ELISA for the determination of polychlorinated biphenyls in soil samples

et al. 2002

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“…In addition, Glass et al [67] reported a less complicated simple two-step liquid-liquid extraction which was used to detect PCBs in transformer oil, resulting in no false negatives at a 1.4 ppm nominal cutoff. Moreover, using a simple oxidation of the sample extract (including 1 mL acetonitrile, 0.5 mL isooctane, 1 mL H 2 SO 4 and 8 mL “Quenching Buffer”) to analyze PCBs in waste oil, ELISA results were highly correlated with the results obtained by instrumental analysis [68]. …”

Section: Detection Methodsmentioning

confidence: 99%

Immunoanalysis Methods for the Detection of Dioxins and Related Chemicals

Tian

Xie

et al. 2012

Sensors

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With the development of biotechnology, approaches based on antibodies, such as enzyme-linked immunosorbent assay (ELISA), active aryl hydrocarbon immunoassay (Ah-I) and other multi-analyte immunoassays, have been utilized as alternatives to the conventional techniques based on gas chromatography and mass spectroscopy for the analysis of dioxin and dioxin-like compounds in environmental and biological samples. These screening methods have been verified as rapid, simple and cost-effective. This paper provides an overview on the development and application of antibody-based approaches, such as ELISA, Ah-I, and multi-analyte immunoassays, covering the sample extraction and cleanup, antigen design, antibody preparation and immunoanalysis. However, in order to meet the requirements for on-site fast detection and relative quantification of dioxins in the environment, further optimization is needed to make these immuno-analytical methods more sensitive and easy to use.

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“…Recently, there has been an increasing use of immunoassays for the detection of environmental contaminants because of their reliability, rapid detection, ease-of-operation, and relatively low cost. 15 During the past two decades, several immunoassays, including the radioimmunoassay, 16,17 ELISA, [18][19][20] the uoroimmunoassay, 21 immunosensor assay, [22][23][24] bioelectrochemical immunoassay, 25,26 real-time quantitative uorescence immuno PCR, [27][28][29] and commercial PCB test kits, have been developed for PCB detection in the environment. Immunoassays are also capable of detecting a wide variety of PCB congeners at submicrogram levels.…”

Section: Introductionmentioning

confidence: 99%