Analysis of pesticides by chemiluminescence detection in the liquid phase

Gámiz‐Gracia, Laura; Garcı́a-Campaña, Ana M.; Soto‐Chinchilla, Jorge J.; Huertas-Pérez, José F.; González‐Casado, Antonio

doi:10.1016/j.trac.2005.05.009

Cited by 112 publications

(41 citation statements)

References 74 publications

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“…Only PIR and CLO gave similar LODs and LOQs with both detection systems. Although greater sensitivity of CL versus DAD determination was expected [9], it should be noted that the optimization process must find a compromise between the CL response for all the pesticides tested. Accordingly, the greater sensitivity value of one is often disregarded in order to have the best results for the whole group.…”

Section: Analytical Characteristicsmentioning

confidence: 99%

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Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV)

Catalá-Icardo

Zamora

Torres-Cartas

et al. 2014

Journal of Chromatography A

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A new, fast, selective and sensitive method has been developed for the simultaneous determination of nine organothiophosphorus (OTP) pesticides, namely omethoate, dimethoate, disulfoton-sulfoxide, methidathion, phosmet, malathion, diazinon, pirimiphos-methyl and chlorpyrifos. The pesticides were separated on a Kinetex C18 column by gradient elution with acetonitrile:water. A post-column basic hydrolysis of the pesticides and later a chemiluminescence (CL) reaction with cerium (IV) in acid medium was carried out. Hexadecylpyridinium chloride highly enhanced the CL emission. Under optimized conditions, linearity, precision, limits of detection and quantification, and accuracy were determined. Both selectivity and sensitivity were compared with those obtained with UV detection. In combination with SPE, limits of detection in the range 15-80 ng/L and 5-30 ng/L were obtained when 250 mL and 1000 mL of solution were treated, respectively. When applied to 250 mL of sample the inter-day precision of the method was between 3.5% and 7.3% and the intra-day precision between 2.9% and 6.0%. The method was applied to determine OTP pesticides in spiked water samples from different origins: irrigation, river, sea, ground, spring, mineral and tap waters, being the percentage of recovery of added amounts near 100% form most of the pesticides.

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Section: Analytical Characteristicsmentioning

confidence: 99%

“…In fact, CL has been gaining increasing acceptance and has been used to determine OPPs residues in recent years [9][10][11][12][13][14][15]. Nevertheless, practical application of the HPLC-CL technique for the determination of pesticides is still uncommon.…”

Section: Introductionmentioning

confidence: 99%

Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV)

Catalá-Icardo

Zamora

Torres-Cartas

et al. 2014

Journal of Chromatography A

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“…Oxidants such as permanganate, hypochlorite, iodine or hydrogen peroxide can be used, being the reaction catalyzed by metal ions, ferricyanide, some metallocomplexes (hemin, hemoglobin) and peroxidases. The two types of oxidants more frequently used with 6 ] has some advantages over H 2 O 2 for its utilization in CE: (i) it comprises the oxidant and the catalyst at the same time, hence the CE-CL configuration is simpler; (ii) there are no problems with disruptions of current due to bubble formation occurring with H 2 O 2 ; (iii) it is soluble in alkaline media and therefore (iv) it is suitable for the luminol reaction (pH 10-11). One possible drawback of the luminol-based CL systems could be that the reaction is only efficient over a narrow pH range and this could limit the pH at which CE separation can be carried out.…”

Section: Luminol and Derivativesmentioning

confidence: 99%

“…The reaction of luminol with K 3 [Fe(CN) 6 ] produces an intense CL emission; therefore, the analytes can have two effects: enhancement or inhibition of the CL emission. Even some analytes, such as rutin or chlorogenic acid, can have both effects depending on the experimental conditions.…”

Section: Clinical and Pharmaceutical Applicationsmentioning

confidence: 99%

“…Due to its simplicity, low cost and high sensitivity and selectivity, CL-based detection has become a quite useful detecting tool in flow injection (FI), column LC and in CE systems, making this technique an interesting field of research, such as clinical [4], pharmaceutical and biomedical [5] or environmental and food analysis [6]. Minimal instrumentation is required and because no external light source is needed, the optical system is quite simple.…”

Section: Introductionmentioning

confidence: 99%

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

2010

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Advances and analytical applications in chemiluminescence coupled to capillary electrophoresisThe present review presents the state of the art of the developments, key strategies and analytical applications of chemiluminescence detection coupled to CE (CE-CL). Different parts considering the most common CL systems have been included, such as the tris(2,2 0 -bipyridine)ruthenium(II) system, the luminol and derivatives reaction, the peroxyoxalate CL or direct oxidations. New advances in homemade configurations and applications in different fields such as clinical, pharmaceutical, environmental and food analysis have been included. The focus is on studies which appeared from 2000 to the end of 2009.

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Non‐enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device

et al. 2008

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A new method for non-enzymatic aqueous peroxyoxalate chemiluminescence (POCL) biomolecular detection using imaging chip-based devices has been developed. A water-soluble amide of oxalic acid was synthesized and used in the investigation and characterization of POCL immunodetection in an aqueous environment. Six fluorescent dyes commonly used in biological detection were tested, and the intensity of light generated from the aqueous POCL reactions was characterized in the liquid phase. Direct detection sensitivity comparisons between a standard fluorescent method and this POCL method were performed in both liquid and solid phases. Results showed that detection sensitivity using the POCL method is comparable to that of the fluorescent method. POCL biomolecular detection on a nitrocellulose membrane was also investigated using a charge-coupled device (CCD) camera. Again, POCL detection sensitivity proved to be comparable to that using the fluorescent detection method. In an application of aqueous POCL biomolecular detection, Staphylococcus aureus enterotoxin B (SEB) and its antibody were used to demonstrate immuno- and affinity detection. For further applications, such as DNA and protein arrays, simultaneous detection of biomolecules labelled with different fluorescent labels was investigated, using a complementary metal oxide semiconductor (CMOS) colour imaging chip.

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Analysis of pesticides by chemiluminescence detection in the liquid phase

Cited by 112 publications

References 74 publications

Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV)

Determination of organothiophosphorus pesticides in water by liquid chromatography and post-column chemiluminescence with cerium(IV)

Advances and analytical applications in chemiluminescence coupled to capillary electrophoresis

Non‐enzymatic aqueous peroxyoxalate chemiluminescence immune detection using a CCD camera and a CMOS device

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