Analysis of phenolic compounds, antioxidant and antimicrobial properties of some endemic medicinal plants

Abstract

This study was designed to evaluate the phenolic compounds and the in vitro antioxidant and antimicrobial activities of Onobrychis nitida, Hedysarum cappadocicum, Ebenus laguroides and Ebenus macrophylla which are medicinal plants and endemic for the flora of Turkey. The RP-HPLC-DAD (reverse phasehigh performance liquid chromatography with a diode array detector) was used to evaluate the phenolic contents. The antioxidant properties were determined to use total phenolic content (TPC), ferric reducing antioxidant power (FRAP), and DPPH• radical scavenging activity assays. Antibacterial tests were performed against 11 different microorganisms by using the microwell dilution method. Each of the plant extracts were confirmed by bioactive assays which demonstrated a significant activity due to different chemical characteristics. Especially, rutin was the dominant component in Ebenus species with 19.434-11.808 mg phenolic/g extract. While the highest phenolic content (101.73 mg gallic acid equivalent/g extract) was observed in O. nitida, the highest FRAP value was in E. laguroides (719.09 μM FeSO4.7H2O equivalent), and the strongest DPPH degree was in the E. macrophylla extract with IC50: 69.45 μg/mL, respectively. Although plant extracts didn't have efficient values for antimicrobial activity, the slight effect was arisen in O. nitida against B. subtilis and S. aureus. The results showed that all the extracts could be used in pharmacological or dietary applications due to their valuable properties.

Antioxidant and Antibacterial Effects of Some Medicinal Plants of Iran

“…The beneficial medicinal effect of a plant is due to the secondary metabolites in the plant [50][51][52][53]. There are alkaloids, flavonoids and phenolic compounds in the methanol extract of E. khuzistanicum flower according to GC/MS analysis that similar results have been described by Tiwari et al (2011) [50].…”

Biological activity, and volatile and phenolic compounds from five Lamiaceae species

“…Plant secondary metabolites can be separated into two major classes by their chemical structure: easy phenols (phenolic acids and coumarins) and polyphenols (flavonoids, stilbenes, lignans, tannins). Due to their biological activity (antibacterial, antifungal, antiviral and antioxidant), phenolic compounds play a significant role in some metabolic pathways 54,55 . The RP‐HPLC with DAD analysis, a common method for the identification of the phenolic compounds in plants, was used to detect major phenolic compounds in the investigated plant extracts.…”

Total phenolic content, antioxidant, antibacterial and antifungal activities, FT-IR analyses of Brassica oleracea L. var. acephala and Ornithogalum umbellatum L.

“…For many centuries, plants have been utilized to heal infectious illnesses and regarded as considerable source of new antimicrobial drugs (BEREKSI et al, 2018). Many studies have been performed to screen the antimicrobial capacities of medicinal plants (BEKTAŞ et al, 2018;KAYA et al, 2018;MOSTAFA et al, 2018).…”