Analysis of phosphatidylcholine in soy lecithins by HPLC

Rhee, Jae-Sung; Shin, Min‐Chol

doi:10.1007/bf02678722

Cited by 19 publications

(3 citation statements)

References 6 publications

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“…A forthcoming method is detection of phospholipid classes by mass spectrometry (MS) (20)(21)(22)(23). Different elution systems have been used for phos- (14,21,25), chloroform/methanol/ water (16,26,27), and acetonitrile/methanol/water (28)(29)(30)(31), most including different buffers in various ratios. Today the analysis of phospholipid-containing material by 31 P NMR is a widespread method.…”

Section: Introductionmentioning

confidence: 99%

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Helmerich¹,

Koehler²

2003

J. Agric. Food Chem.

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Phospholipid classes were determined qualitatively and quantitatively in eight commercial lecithins and three flour improvers by thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), and (31)P nuclear magnetic resonance spectroscopy ((31)P NMR). The total amounts of phospholipids as well as the amounts of phospholipid classes in the samples were comparable but depended on the method used for quantification. Highest selectivity was provided by (31)P NMR as all phospholipids and lysophospholipids could easily be quantified. By TLC only lysophosphatidylcholine could not be quantified, whereas HPLC was the method with the lowest selectivity, because lysophospholipids, except lysophosphatidylethanolamine, could not be determined. Sensitivity was best for HPLC and TLC with detection limits of 20-170 mug/mL. By means of (31)P NMR these figures increased by a factor of 10-70. The coefficients of variation were 5.5, 6.8, and 12.8% for quantification by TLC, HPLC, and (31)P NMR, respectively, showing that TLC was the method with the best reproducibility. Altogether, (31)P NMR can be recommended for the quantification of phospholipids, because it is easy to perform and results can be obtained quickly. As it requires minimum instrumental equipment, TLC is a good alternative to (31)P NMR. If high sensitivity is required, HPLC is the best method.

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Section: Introductionmentioning

confidence: 99%

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Helmerich¹,

Koehler²

2003

J. Agric. Food Chem.

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“…Perhaps the most commonly reported separations by HPLC have been monoacylglycerols (Filip and Kleunova, 1993;Takagi and Ando, 1994;Ranger and Wenz, 1989;Tajano and Kondoh, 1987;Martin et al, 1989;Rilsom and Hoffmayer, 1978;Brueschweiler, 1977;Firestone, 2005aj) and phospholipids (Christie, 1996;Melton, 1992;Sotirhos et al, 1986;Hurst and Martin, 1984;Huyghebaert and Baert, 1992;Tumanaka and Fujita, 1990;Rhee and Shin, 1982;Hsieh et al, 1981;Kaitaranta and Bessman, 1981 p. 5;Firestone, 2005aj;Luquain et al, 2001). Free glycerine may also be determined by HPLC (Firestone, 2005ak).…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Food Emulsifiers and Their Applications

2008

120

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“…Many reports on the HPLC method for PC analysis have been released. However, since most of the reports use UV as a detector, measured values are affected by differences in fatty acid composition, particularly if unsaturated fatty acids are present in the composition of PC (5,6). On the other hand, the values obtained by the RI-detector are hardly affected by differences in fatty acid composition.…”

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confidence: 99%

Quantitative Determination of phosphatidylcholine by an HPLC‐RI system

Yamagishi

Akiyama

Kimura

et al. 1989

J Americ Oil Chem Soc

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The following describes the quantitative determining method for phosphatidylcholine (PC) using the HPLC-RI system which we have developed. It uses Lichrosorb, Si 60 (10 pro), 4.6 mm X 250 mm as the column and a mobile phase consisting of n-hexane/isopropanol/water ---1:4:1. In this report, we compared data from selected highpurity (60-100 wt%) samples using the HPLC-RI, HPLC-UV and conventional TLC-P methods.Under the conditions we described, the HPLC-UV method was somewhat affected by fatty acid compositions. As a result, there were some inconsistencies in the measured values. However, the HPLC-RI method we propose was applicable to PC from both egg yolk and soybeans. In addition, the HPLC-RI method produced data which correlated well with data from the TLC-P method, and this data was highly accurate and exhibited satisfactory reproducibility.

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Analysis of phosphatidylcholine in soy lecithins by HPLC

Abstract: A simple and rapid high pressure liquid chromatographic method with RI detector was developed to determine the content of phos‐phatidylcholine in soy lecithins.

Cited by 19 publications

References 6 publications

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Comparison of Methods for the Quantitative Determination of Phospholipids in Lecithins and Flour Improvers

Food Emulsifiers and Their Applications

Quantitative Determination of phosphatidylcholine by an HPLC‐RI system

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