2006
DOI: 10.1016/j.jpba.2006.02.052
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Analysis of phytosterols in foods

Abstract: Phytosterols are bioactive compounds, one of their most studied and outstanding properties being their cholesterol-lowering activity. This explains the growing interest in the phytosterol contents of foods as either intrinsic or added components. The different steps (extraction, saponification, clean up, chromatographic determination) of plant sterol determination are reviewed, and emphasis is placed on the methods used to assay different phytosterols in food.

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Cited by 279 publications
(236 citation statements)
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“…While they lower the levels of LDL-cholesterol, levels of 'good' HDL-cholesterol and triacyglycerols remain unaffected [74]. The beneficial health effects of plant sterols have led to the development of functional foods enriched in sterols.…”
Section: Phytosterolsmentioning
confidence: 99%
“…While they lower the levels of LDL-cholesterol, levels of 'good' HDL-cholesterol and triacyglycerols remain unaffected [74]. The beneficial health effects of plant sterols have led to the development of functional foods enriched in sterols.…”
Section: Phytosterolsmentioning
confidence: 99%
“…The main proteins are two globulins, arachine and conarchine, that represent at least 95% of the total seed proteins (Yoshida et al, 1995;Andersen et al,1998;Han et al, 2009). Phytosterols and triterpenes make up the greatest proportion of the unsaponifi able components of vegetable oils (Azadmard-Damirchi et al, 2005); they are considered to have anti-inflammatory, anti-bacterial, antiartherosclerotic, antioxidative (Lagarda et al, 2006), antiulcerative and antitumor properties in humans (Moreau et al, 2002), as well as contributing to the oxidative and thermal stability and shelf-life of vegetable oils (Beveridge et al, 2002;Przybylski andEskin, 2006, Alvarenga andEsteban, 2005). Peanut seeds have high nutritional and commercial value due to their protein, fatty acid, carbohydrate and fi ber content, as well as vitamins, calcium, and phosphorous.…”
Section: Introductionmentioning
confidence: 99%
“…Methanolic solutions of known gallic acid concentrations in the range of 0-250 mg L −1 were used for calibration. The results were expressed as mg gallic acid equivalents (GAE) 100 g −1 fresh weight (FW) using the calibration curve (equation 4): y = −0.0273 + 0.00517x, r 2 = 0.999 (4) Tocopherol and phytosterol analysis Phytosterols and tocopherols were assessed by the saponification of 50-100 mg of extracted oil. 20 The saponification was started with 1.0 mL of 1 mol L −1 KOH in ethanol.…”
Section: Phenolic Compoundsmentioning
confidence: 99%
“…2,3 Two hundred kinds of phytosterols are present in vegetables; β-sitosterol (24-α-ethylcholesterol), campesterol (24-α-mathylcholesterol), and stigmasterol (∆ 22 , 24-α-ethylcholesterol) are the most abundant. 4,5 Tocopherol is a natural component present in vegetable products and it has antioxidant activity against reactive oxygen species (ROS) and it can offer health benefits against the development of chronic diseases. 6,7 Thus, phytosterols and tocopherols compounds have important function on antioxidant, anti-inflammatory, antitumor, and enhanced immunity action, together with other biological activities.…”
Section: Introductionmentioning
confidence: 99%