Analysis of PLA/PHB Biopolymer Material with Admixture of Hydroxyapatite and Tricalcium Phosphate for Clinical Use

Kohan, Miroslav; Lancoš, Samuel; Schnitzer, Marek; Živčák, Jozef; Hudák, Radovan

doi:10.3390/polym14245357

Cited by 8 publications

(6 citation statements)

References 53 publications

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“…The results showed that all samples were safe, resulting in cell viability higher than 70%, similar to control ( Figure 7 ). This result confirmed previous ones that demonstrated no toxicity of biocomposites based on PHB/BC, and PHB blended with different biopolymers, including PLA and Hydroxyapatite (HA) [ 62 , 63 ]. Furthermore, the PHB/BC-based biocomposites proved to support 3T3-L1 preadipocyte proliferation and induced a positive effect on osteoblast differentiation in vivo.…”

Section: Resultssupporting

confidence: 91%

3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications

Celestino,

Lima,

Fontes

et al. 2023

JFB

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In this work, scaffolds based on poly(hydroxybutyrate) (PHB) and micronized bacterial cellulose (BC) were produced through 3D printing. Filaments for the printing were obtained by varying the percentage of micronized BC (0.25, 0.50, 1.00, and 2.00%) inserted in relation to the PHB matrix. Despite the varying concentrations of BC, the biocomposite filaments predominantly contained PHB functional groups, as Fourier transform infrared spectroscopy (FTIR) demonstrated. Thermogravimetric analyses (i.e., TG and DTG) of the filaments showed that the peak temperature (Tpeak) of PHB degradation decreased as the concentration of BC increased, with the lowest being 248 °C, referring to the biocomposite filament PHB/2.0% BC, which has the highest concentration of BC. Although there was a variation in the thermal behavior of the filaments, it was not significant enough to make printing impossible, considering that the PHB melting temperature was 170 °C. Biological assays indicated the non-cytotoxicity of scaffolds and the provision of cell anchorage sites. The results obtained in this research open up new paths for the application of this innovation in tissue engineering.

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Section: Resultssupporting

confidence: 91%

3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications

Celestino,

Lima,

Fontes

et al. 2023

JFB

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“…The results of the experiment present knowledge about their suitability for clinical use in the human body. A similar topic was addressed by Kohan et al [ 23 ], who performed cytotoxicity and cell proliferation tests on PLA/PHB + HA/TCP polymers using L929 mouse fibroblasts. The results showed that the materials are not cytotoxic, and the metabolic activity of the cells is greater than 70% in all cases.…”

Section: Discussionmentioning

confidence: 83%

“…They investigated mixtures of PLA and PHB materials at different mass ratios, which helped us in the analysis of SEM images. Similarly, Kohan et al [ 23 ] observed the distribution of the ceramic component in the PLA/PHB composite using SEM analysis. Through SEM, Čulenová et al [ 13 ] observed the surface of the PLA/PHB/TPS material and the visible concentration of the cell distribution.…”

Section: Discussionmentioning

confidence: 99%

“…Filaments were manufactured at the Department of Biomedical Engineering, Technical University in Košice, according to the study by Kohan et al, 2022 [ 23 ]. Each type of the compared mixed polymer materials was prepared in the form of granules with a weight of approximately 10 g. In this form, the material was placed in the moisture analyzer Radwag 50/1 (RADWAG, Radom, Poland), where it was dried.…”

Section: Methodsmentioning

confidence: 99%

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Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers

Trebuňová,

Petroušková,

Balogová

et al. 2023

JFB

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One of the blends that is usable for 3D printing while not being toxic to cell cultures is the lactic acid (PLA)/polyhydroxybutyrate (PHB)/thermoplastic starch (TPS) blend. The addition of plasticizers can change the rate of biodegradation and the biological behavior of the material. In order to evaluate the potential of the PLA/PHB/TPS material in combination with additives (plasticizers: acetyl tributyl citrate (ATBC) and oligomeric lactic acid (OLA)), for use in the field of biomedical tissue engineering, we performed a comprehensive in vitro characterization of selected mixture materials. Three types of materials were tested: I: PLA/PHB/TPS + 25% OLA, II: PLA/PHB/TPS + 30% ATBC, and III: PLA/PHB/TPS + 30% OLA. The assessment of the biocompatibility of the materials included cytotoxicity tests, such as monitoring the viability, proliferation and morphology of cells and their deposition on the surface of the materials. The cell line 7F2 osteoblasts (Mus musculus) was used in the experiments. Based on the test results, the significant influence of plasticizers on the material was confirmed, with their specific proportions in the mixtures. PLA/PHB/TPS + 25% OLA was evaluated as the optimal material for biocompatibility with 7F2 osteoblasts. The tested biomaterials have the potential for further investigation with a possible change in the proportion of plasticizers, which can have a fundamental impact on their biological properties.

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“…The extruder temperature profiles were adjusted to 170, 180, 180, and 195 • C while maintaining a screw speed of 50-60 rpm. The resulting extrudate was cooled through a dual air-cooling system to produce a filament with a diameter of 1.75 mm [38]. The morphology of the generated DCP-PHB/PMMA filament was analysed using a Scanning Electron Microscope (SEM) (HITACHI S-4700, Tokyo, Japan).…”

Section: Filament Fabrication and Sem Analysismentioning

confidence: 99%

Sustainable Polyhydroxyalkanoate Production from Food Waste via Bacillus mycoides ICRI89: Enhanced 3D Printing with Poly (Methyl Methacrylate) Blend

Rofeal,

Abdelmalek,

Pietrasik

2023

Polymers

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In view of implementing green technologies for bioplastic turning polices, novel durable feedstock for Bacillus mycoides ICRI89 used for efficient polyhydroxybutyrate (PHB) generation is proposed herein. First, two food waste (FW) pretreatment methods were compared, where the ultrasonication approach for 7 min was effective in easing the following enzymatic action. After treatment with a mixture of cellulase/amylases, an impressive 25.3 ± 0.22 g/L of glucose was liberated per 50 g of FW. Furthermore, a notable 2.11 ± 0.06 g/L PHB and 3.56 ± 0.11 g/L cell dry eight (CDW) over 120 h were generated, representing a productivity percentage of 59.3 wt% using 25% FW hydrolysate. The blend of polyhydroxybutyrate/poly (methyl methacrylate) (PHB/PMMA = 1:2) possessed the most satisfactory mechanical properties. For the first time, PHB was chemically crosslinked with PMMA using dicumyl peroxide (DCP), where a concentration of 0.3 wt% had a considerable effect on increasing the mechanical stability of the blend. FTIR analysis confirmed the molecular interaction between PHB and PMMA showing a modest expansion of the C=O stretching vibration at 1725 cm−1. The DCP-PHB/PMMA blend had significant thermal stability and biodegradation profiles comparable to those of the main constituent polymers. More importantly, a 3-Dimetional (3D) filament was successfully extruded with a diameter of 1.75 mm, where no blockages or air bubbles were noticed via SEM. A new PHB/PMMA “key of life” 3D model has been printed with a filling percentage of 60% and a short printing time of 19.2 min. To conclude, high-performance polymeric 3D models have been fabricated to meet the pressing demands for future applications of sustainable polymers.

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Analysis of PLA/PHB Biopolymer Material with Admixture of Hydroxyapatite and Tricalcium Phosphate for Clinical Use

Cited by 8 publications

References 53 publications

3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications

3D Filaments Based on Polyhydroxy Butyrate—Micronized Bacterial Cellulose for Tissue Engineering Applications

Evaluation of Biocompatibility of PLA/PHB/TPS Polymer Scaffolds with Different Additives of ATBC and OLA Plasticizers

Sustainable Polyhydroxyalkanoate Production from Food Waste via Bacillus mycoides ICRI89: Enhanced 3D Printing with Poly (Methyl Methacrylate) Blend

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