Analysis of Potato Glycoalkaloids by High‐performance Liquid Chromatography

Bushway, Rodney J.; Barden, E. S.; Wilson, A. M.; Bushway, Alfred A.

doi:10.1111/j.1365-2621.1980.tb07528.x

Cited by 31 publications

(9 citation statements)

References 10 publications

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“…Friedman and others (2003) reported glycoalkaloid content of 8 varieties contained about 630 mg and 3500 mg/kg of dehydrated whole potato and peel, respectively. Previously, Bushway and others (1980) reported that α‐chaconine and α‐solanine concentration varied from 3.89 to 23.04 mg, 1.69 to 12.51 mg/100 g in flesh portion of potato among different potato cultivars, respectively (Fitzpatrick and Osman 1974).…”

Section: Resultsmentioning

confidence: 99%

Glycoalkaloids (α‐Chaconine and α‐Solanine) Contents of Selected Pakistani Potato Cultivars and Their Dietary Intake Assessment

Aziz

Randhawa

Butt

et al. 2012

Journal of Food Science

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Glycoalkaloids (α-solanine and α-chaconine) are naturally occurring toxic compounds in potato tuber (Solanum tuberosum L.) that cause acute intoxication in humans after their consumption. Present research was conducted to evaluate α-chaconine, α-solanine, and total glycoalkaloids (TGAs) contents in the peel and flesh portions by high-performance liquid chromatography method in selected Pakistani potato cultivars. The α-solanine content varies 45.98 ± 1.63 to 2742.60 ± 92.97 mg/100 g of dry weight (DW) in peel and from 4.01 ± 0.14 to 2466.56 ± 87.21 mg/100 g of DW in flesh. Similarly, α-chaconine content varied from 4.42 ± 0.16 to 6818.40 ± 211.07 mg/100 g of DW in potato peel and from 3.94 ± 0.14 to 475.33 ± 16.81 mg/100 g DW in flesh portion. The TGA concentration varied from 177.20 ± 6.26 to 5449.90 ± 192.68 mg/100 g of DW in peel and from 3.08 ± 0.11 to 14.69 ± 0.52 mg/100 g of DW in flesh portion of all the potato cultivars tested. All the potato cultivars contained lower concentration of TGA than the limits recommended as safe, except 2 cultivars, that is FD8-3 (2539.18 ± 89.77 mg/100 g of DW) and Cardinal (506.16 ± 17.90 mg/kg). The dietary intake assessment of potato cultivars revealed that Cardinal, FD 35-36, FD 8-3, and FD 3-9 contained higher amount of TGA in whole potato, although FD 8-3 only possessed higher content of TGA (154.93 ± 7.75) in its flesh portion rendering it unfit for human consumption. Practical Application: This paper was based on the research conducted on toxic compounds present in all possible potato cultivars in Pakistan. Actually, we quantify the toxic compounds (glycoalkaloids) of potato cultivars through HPLC and their dietary assessment. This paper revealed safety assessment and their application in food industries especially potato processing.

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Section: Resultsmentioning

confidence: 99%

Glycoalkaloids (α‐Chaconine and α‐Solanine) Contents of Selected Pakistani Potato Cultivars and Their Dietary Intake Assessment

Aziz

Randhawa

Butt

et al. 2012

Journal of Food Science

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“…enough concentrated ammonia to raise the pH to 11.0. The ammoniacal extract was then warmed to 70 °C and cooled overnight at 4 °C, and the precipitate was collected by centrifugation (Bushway et al, 1980). After the pellet was washed twice with 1% ammonia and dried (16 h at 70 °C in a vacuum oven), the glycoalkaloids were dissolved in an appropriate volume of methanol, filtered, and, following acid hydrolysis, titrated with bromophenol blue in 10% phenol (Fitzpatrick and Osman, 1974).…”

Section: Methodsmentioning

confidence: 99%

Glycoalkaloids of wild, tuber-bearing Solanum species

Gregory¹,

Sinden²,

Osman³

et al. 1981

J. Agric. Food Chem.

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The total glycoalkaloid (TGA) levels and individual glycoalkaloid compositions of current potato cultivare do not represent a toxicological or teratogenic hazard. However, the widespread exploitation of wild, tuber-bearing Solanum species in potato breeding programs suggests the need for caution in the future. Some species contain much higher TGA levels than are found in Solanum tuberosum (Schreiber, 1963; Osman et al., 1978). There is also widespread occurrence among these wild species of glycoalkaloids which are not commonly associated with commercial varieties [for a review, see Schreiber (1968)]. Since TGA levels and individual glycoalkaloid compositions are largely under genetic control (Sanford and Sinden, 1972;McCollum and Sinden, 1979), and glycoalkaloids have been associated with undesirable potato flavor (Sinden and Deahl, 1976), mammalian toxicity and teratogenicity [for a review, see Kuc (1975)], careful consideration of glycoalkaloid contents in the parental species is highly desirable.The need for this was illustrated in the late 1960s when a Gr. Tuberosum cultivar Lenape, which had S. chacoense in its ancestry (Akeley et al., 1968

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“…(1985), andvanGelder (1991). High-performance liquid chromatography (HPLC) has been reported by several authors in the separation and quantitation of some major GA (Bushway et al, 1980(Bushway et al, ,1986Crabbe and Fryer, 1980;Hunter et al, 1980;Morris and Lee, 1981; Carmen et al, 1986;Hellenes, 1986;Kobayashi et al, 1989;Saito et al, 1990). GA do not have a suitable UV chromaphore, and therefore absorbance is measured at 208 nm, where many compounds absorb light.…”

Section: Introductionmentioning

confidence: 99%