Analysis of Proapoptotic Protein Trafficking to and from Mitochondria

Vega‐Naredo, Ignacio; Oliveira, Gabriela; Cunha‐Oliveira, Teresa; Serafim, Teresa L.; Sardão, Vilma A.; Oliveira, Paulo J.

doi:10.1007/978-1-0716-1433-4_10

Cited by 2 publications

(2 citation statements)

References 45 publications

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“…Mitochondria play a key role in cell death and its regulation, and Bcl-2 family proteins control mitochondrial outer membrane permeability by regulating membrane potential ( 34 ). The imbalance of Bcl-2 family expression results in the release of proapoptotic factors into the cytoplasm, thereby inducing apoptosis ( 35 ).…”

Section: Discussionmentioning

confidence: 99%

Atlas of metabolism reveals palmitic acid results in mitochondrial dysfunction and cell apoptosis by inhibiting fatty acid β-oxidation in Sertoli cells

Luo²,

Xuan³

et al. 2022

Front. Endocrinol.

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In recent years, the impact of lipotoxicity on male fertility has received extensive attention, especially on Sertoli cells (SCs). In SCs, energy metabolism is important as disorders of energy metabolism result in infertility eventually. However, the underlying mechanism of lipotoxicity on energy metabolism in SCs remains unknown. Advances in high-throughput metabolomics and lipidomics measurement platforms provide powerful tools to gain insights into complex biological systems. Here, we aimed to explore the potential molecular mechanisms of palmitic acid (PA) regulating energy metabolism in SCs based on metabolomics and lipidomics. The results showed that glucose metabolism-related metabolites were not significantly changed, which suggested that PA treatment had little effect on glucose metabolism and may not influence the normal energy supply from SCs to germ cells. However, fatty acid β-oxidation was inhibited according to accumulation of medium- and long-chain acylcarnitines in cells. In addition, the pool of amino acids and the levels of most individual amino acids involved in the tricarboxylic acid (TCA) cycle were not changed after PA treatment in SCs. Moreover, PA treatment of SCs significantly altered the lipidome, including significant decreases in cardiolipin and glycolipids as well as remarkable increases in ceramide and lysophospholipids, which indicated that mitochondrial function was affected and apoptosis was triggered. The increased apoptosis rate of SCs was verified by elevated protein expression levels of Cleaved Caspase-3 and Bax as well as decreased Bcl-2 protein expression level. Together, these findings indicated that PA may result in mitochondrial dysfunction and increased apoptosis by inhibiting fatty acid β-oxidation of SCs.

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Section: Discussionmentioning

confidence: 99%

Atlas of metabolism reveals palmitic acid results in mitochondrial dysfunction and cell apoptosis by inhibiting fatty acid β-oxidation in Sertoli cells

Luo²,

Xuan³

et al. 2022

Front. Endocrinol.

View full text Add to dashboard Cite

show abstract

“…After being regulated by apoptosis-related gene B cell lymphoma-2 (Bcl-2), the Caspase family can directly destroy cell structure and induce mitochondrial apoptosis (Häcker, 2018). Related studies have found that Smac/DIABLO and Omi/HtrA2 are present in mitochondria, which can be released from mitochondria into cytoplasm when the cell structure is disrupted, thus serving as a marker of mitochondrial apoptosis (Grosser et al, 2021;Vega-Naredo et al, 2021). Toll-like receptor 4 (TLR4) signalling is a classical pathway of polysaccharide mediated immunoregulation (Li et al, 2022;Zhao et al, 2022).…”

Section: Immunomodulatory Activitymentioning

confidence: 99%

The chemistry and efficacy benefits of polysaccharides from Atractylodes macrocephala Koidz

et al. 2022

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Atractylodes macrocephala Koidz (AM), traditional Chinese medicine (TCM) with many medicinal values, has a long usage history in China and other oriental countries. The phytochemical investigation revealed the presence of volatile oils, polysaccharides, lactones, flavonoids, and others. The polysaccharides from AM are important medicinal components, mainly composed of glucose (Glc), galactose (Gal), rhamnose (Rha), arabinose (Ara), mannose (Man), galacturonic acid (GalA) and xylose (Xyl). It also showed valuable bioactivities, such as immunomodulatory, antitumour, gastroprotective and intestinal health-promoting, hepatoprotective, hypoglycaemic as well as other activities. At the same time, based on its special structure and pharmacological activity, it can also be used as immune adjuvant, natural plant supplement and vaccine adjuvant. The aim of this review is to summarize and critically analyze up-to-data on the chemical compositions, biological activities and applications of polysaccharide from AM based on scientific literatures in recent years.

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Analysis of Proapoptotic Protein Trafficking to and from Mitochondria

Cited by 2 publications

References 45 publications

Atlas of metabolism reveals palmitic acid results in mitochondrial dysfunction and cell apoptosis by inhibiting fatty acid β-oxidation in Sertoli cells

Atlas of metabolism reveals palmitic acid results in mitochondrial dysfunction and cell apoptosis by inhibiting fatty acid β-oxidation in Sertoli cells

The chemistry and efficacy benefits of polysaccharides from Atractylodes macrocephala Koidz

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