Analysis of protonated and alkali metal cationized aminoglycoside antibiotics by collision-activated dissociation and infrared multi-photon dissociation in the quadrupole ion trap

Goolsby, Brian; Brodbelt, Jennifer S.

doi:10.1002/1096-9888(200008)35:8<1011::aid-jms29>3.0.co;2-r

Cited by 34 publications

(33 citation statements)

References 46 publications

(62 reference statements)

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“…142 Da for gentamicin C 2 , C 2a , C 2b ). Another typical fragment formed by the loss of 117 Da from the garosamine sugar has been reported [46][47][48]. The [M+H-117] + ion could be further fragmented to the ion at m/z 205 by the loss of ring A. Glycoside bond cleavage between ring A and B yields the ion m/z 322 (ring B and C, garamine) by the loss of ring A (e.g.…”

Section: Fragmentation Behavior Of Gentamicin Componentsmentioning

confidence: 99%

Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry

Zheng

Wang

et al. 2011

Sci. China Chem.

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Several gentamicin bulk samples from different origins were investigated using an LC/MS method. LC equipped with ion trap MS with positive ionization was performed on a Capcell Pak C 18 (AQ) column with the mobile phase containing 50 mM trifluoroacetic (TFA) and methanol. Impurities present in batches of gentamicin bulk samples were elucidated and compared according to their fragmentation behavior. In total seventeen impurities present in samples, five impurities were not elucidated and two compounds were identified preliminarily. It was observed that the impurity profiles were different in samples from different origins which indicate necessity in the quality control of gentamicin. gentamicin, liquid chromatography, LC/MS, impurity analysis

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Section: Fragmentation Behavior Of Gentamicin Componentsmentioning

confidence: 99%

Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry

Zheng

Wang

et al. 2011

Sci. China Chem.

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“…1). More recently, collisionally activated dissociation of the protonated tobramycin enabled identification of two new compounds in tobramycin sample [10]. More recently, collisionally activated dissociation of the protonated tobramycin enabled identification of two new compounds in tobramycin sample [10].…”

Section: Introductionmentioning

confidence: 99%

Development and validation of capillary electrophoresis method for tobramycin with precapillary derivatization and UV detection

et al. 2002

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One of the major drawbacks in the analysis of aminoglycoside antibiotics is their lack of UV chromophore and/or fluorophore. Tobramycin, a representative member of this group, was examined in this study. To overcome the detection hurdle, a precapillary derivatization followed by capillary electrophoresis analysis with direct UV detection was investigated. A central composite design was applied to optimize the method and three parameters were selected in this study: buffer pH, temperature and % acetonitrile (ACN). Selectivity between tobramycin main component and its adjacent peaks as well as the peak efficiency and symmetry factors were established as responses. For each response, a model was obtained by a second-order mathematical expression. Successful results were obtained with a simple background electrolyte (BGE) containing 30 mM sodium tetraborate, pH 10.2, and ACN (75:25 v/v). Under these conditions, baseline separation of tobramycin from its adjacent kanamycin B and an unknown peak was achieved. A temperature of 20 degrees C and applied voltage of 28.0 kV were used. The method showed good validation data in terms of precision, limits of quantitation and detection, specificity and linearity and was found to be suitable for analysis of tobramycin bulk pharmaceutical samples.

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“…This spectrum also confirmed five phosphorylation sites. Positive ions other than those formed from proteins and peptides have also been subjected to study with ECD, including peptide nucleic acids (PNAs), 167 DNA, 168 oligosaccharides 169 and synthetic polymers. 170,171 An ECD spectrum of polypropylene glycol (PPG) ions [H C 3 H 6 O 82 OH C nH] nC is shown in Fig.…”

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confidence: 99%

Charge permutation reactions in tandem mass spectrometry

McLuckey

2004

J. Mass Spectrom.

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Central to the tandem mass spectrometry experiment is the process that gives rise to product ions, i.e. the reaction intermediate to stages of mass analysis. Changes in mass or charge of the parent ion (or both) are generally readily detected by all forms of tandem mass spectrometry. Charge changing, or charge permutation, reactions have a long history in mass spectrometry. However, with the advent of new ionization methods, such as electrospray ionization, and the expansion of tandem mass spectrometry instrumentation to include ion trapping instruments, the past decade has seen a major increase in the types of charge permutation reactions that can be studied. Most charge permutation reactions involve electrons or protons as the charge mediating agents. This report, therefore, provides an overview of charge permutation reactions involving protons or electrons. Particular emphasis is placed on processes that involve interactions of precursor ions with gaseous neutral species, electrons or oppositely charged ions. Charge permutation reactions involving electron gain/loss are described first according to a rough order of the energy required for the reaction beginning with the most endoergic reactions and ending with the most exoergic reactions. An analogous approach is then taken with charge permutation reactions involving proton gain/loss. Important charge permutation reactions discussed herein, among others, include those referred to as charge inversion, charge stripping, electron capture dissociation, collision-induced ionization and charge separation. These reaction types, and others described herein, are the subjects of active research and are also finding use in many current areas of application.

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Analysis of protonated and alkali metal cationized aminoglycoside antibiotics by collision-activated dissociation and infrared multi-photon dissociation in the quadrupole ion trap

Cited by 34 publications

References 46 publications

Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry

Impurity analysis of gentamicin bulk samples by improved liquid chromatography-ion trap mass spectrometry

Development and validation of capillary electrophoresis method for tobramycin with precapillary derivatization and UV detection

Charge permutation reactions in tandem mass spectrometry

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