Analysis of radioactive phosphates in extracts of cerebral tissues

Heald, P. J.

doi:10.1042/bj0630235

Cited by 30 publications

(14 citation statements)

References 22 publications

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“…Thus, up to 40% of the volume in these slices may be composed of disrupted cells. ATP levels near in vivo values have been reported for cortical slices with only one cut edge Ibata et al, 19711, while ATP levels similar to those found in this study were observed in cortical slices with two cut surfaces (Heald, 1956;Cohen, 1962).…”

Section: Discussionsupporting

confidence: 90%

“…ATP, PCr, total adenylate, total creatine, and cyclic nucleotide levels were all reduced by approximately 50% in the hippocampal slices. This has been noted previously by McIlwain and others (McIlwain et al, 1951;Thomas, 1956Thomas, , 1957Heald, 1956;McIlwain, 1975), who attempted to approxi-mate more closely the in vivo values with media additives. Calculated values such as energy charge and the PCr/ATP ratio are independent of brain slice protein content and resemble in vivo values.…”

Section: Discussionsupporting

confidence: 56%

“…levels have been reported t o be roughly half of in vivo values (McIlwain e t al., 1951;Heald, 1956; Thomas, 1956Thomas, , 1957Cohen, 1962). Finally, brain slices show a marked redistribution of cations, with a lower intracellular K + / N a + ratio (Keesey e t al., 1965;Franck et al, 1968).…”

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confidence: 99%

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Metabolic Stability of Hippocampal Slice Preparations During Prolonged Incubation

et al. 1984

Journal of Neurochemistry

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Hippocampal slices were prepared under three conditions: (1) in medium containing glucose and oxygen at 4 degrees C; (2) as in (1), but at 37 degrees C; (3) in medium devoid of glucose and oxygen at 37 degrees C. The rates of recovery to roughly steady-state levels and through 8 h of incubation were monitored for energy metabolite levels and related parameters. In vitro stable values are compared with in situ hippocampal levels. Regardless of the conditions under which slices were prepared, metabolite levels required up to 3 h to stabilize, and these levels were maintained or improved through 8 h of incubation. Further, the maximal concentrations of metabolites were independent of the conditions of slice preparation. Total adenylates and total creatine levels reached 55% of those in vivo. Lactate decreased from the decapitation-induced high levels, but stabilized at concentrations about twice those in rapidly frozen brain. Cyclic AMP and cyclic GMP exhibited peak levels at 30 min of incubation, and cyclic GMP remained elevated for 3 h. Although all three methods of slice preparation resulted in similar metabolite profiles on incubation, the initial decreases in high energy phosphates were delayed by chilling. Most striking, the slices prepared in the absence of glucose and oxygen exhibited much smaller orthodromic evoked potentials in the dentate gyrus. The presence of glucose and oxygen during preparation of the slices appears to be critical to the electrophysiological response of the tissue.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 56%

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Metabolic Stability of Hippocampal Slice Preparations During Prolonged Incubation

et al. 1984

Journal of Neurochemistry

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“…Adeno8ine tripho8phate, creatine phlophate and gluco8e 1-pho8phate. These were samples described previously (Heald, 1956).…”

Section: Methodsmentioning

confidence: 99%

“…During an investigation of the rapid changes in slices of cerebral cortex of guinea pigs brought about by electrical pulses (Heald, 1954) it was found that the rate of breakdown of creatine phosphate appeared to be higher than that required to provide the 'energy-rich' phosphate involved in the metabolism of glucose. From this it was suggested that the 'energy-rich' phosphate released in response to pulses was not used solely in the phosphorylation of glucose.…”

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confidence: 99%