Analysis of Radiomodulatory Effect of Low-Level Laser Irradiation by Clonogenic Survival Assay

Djavid, Gholamreza Esmaeeli; Goliaie, Bahram; Nikoofar, Alireza

doi:10.1089/pho.2015.3893

Cited by 19 publications

(14 citation statements)

References 47 publications

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“…Further studies should be performed to address these issues. LLLT may also selectively inhibit cancer cell proliferation (64) or enhance tumor cell killing with RT (63). This imply that LLLT may have a role as an adjuvant to RT to enhance cancer treatment and be incorporated into the RT treatment plan.…”

Section: Bridging Laboratory Findings To Clinical Applicationsmentioning

confidence: 99%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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Photobiomodulation (PBM) using low-level laser therapy (LLLT) is a treatment that is increasingly used in oncology. Studies reported enhancement of wound healing with reduction in pain, tissue swelling and inflammatory conditions such as radiation dermatitis, oral mucositis, and lymphedema. However, factors such as wavelength, energy density and irradiation frequency influence the cellular mechanisms of LLLT. Moreover, the effects of LLLT vary according to cell types. Thus, controversy arose as a result of poor clinical response reported in some studies that may have used inadequately planned treatment protocols. Since LLLT may enhance tumor cell proliferation, these will also need to be considered before clinical use. This review aims to summarize the current knowledge of the cellular mechanisms of LLLT by considering its effects on cell proliferation, metabolism, angiogenesis, apoptosis and inflammation. With a better understanding of the cellular mechanisms, bridging findings from laboratory studies to clinical application can be improved.

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Section: Bridging Laboratory Findings To Clinical Applicationsmentioning

confidence: 99%

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Tam

Ramkumar

et al. 2020

Front. Oncol.

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“…1 It is broadly applied to reduce inflammation, 2 promote tissue regeneration, 3 relieve pain 4 enhance healing process of wound 5,6 and increase cell proliferation. 7,8 The molecular mechanisms of biological responses after LLL exposure have not been fully demonstrated. It is believed that physiological effects induced by LLL depend on the absorption of energy by some molecular photoacceptors or chromophores in the target cells.…”

Section: Introductionmentioning

confidence: 99%

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

et al. 2017

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Introduction: In recent years, low-power lasers have been widely used in medicine. With the introduction of affordable light emitted diode (LED), clinical application of LED light has become more and more popular. However, some researchers believe that due to lack of coherence of the LED light, it can be different in terms of biological effects, in comparison with laser. In this study, the biological effects of low-level laser (LLL) to those of LED light are compared and discussed. Methods: Human skin fibroblast cell line Hu02 was irradiated with LLL and LED light with a wavelength of 660 nm, power output of 35 mW and in continuous mode and the control group was not irradiated. The biological effects were compared through analysis of cell proliferation, production of reactive oxygen species (ROS) within the cell and rate of cell division. Results: Our findings showed that production of ROS within the cell was linearly increased both in the LED and laser light irradiated cells. However, laser light is more incremental in comparison to LED light. The MTT results showed that laser light at low energy density (less than 5 J/cm 2 ) increased the rate of cell proliferation after 24 hours. Although, the rate of cell division was increased in energy density of 1 J/cm 2 compared to the control group, this increase was not statistically significant. Discussion: The findings indicated that the coherence properties of laser light provided more energy for the cells, and in a constant energy density, laser light created more oxidative stresses in comparison with LED light.

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“…Previous works showed that LLLT can prevent or mitigate the deleterious effects of the ionizing radiation , however, it is unclear whether LLLT could mitigate the damage only in healthy cells or if the laser radiation would also promote tumor cell recovery after IR. Our results showed different behavior of fibroblasts and tumor cells treated with IR followed by LLLT.…”

Section: Discussionmentioning

confidence: 99%

“…On the contrary, low‐level laser therapy (LLLT) using red and near infrared wavelengths is a non‐ionizing radiation that has been reported to modulate harmful effects of the IR in vitro and in vivo . In fact, in vitro studies show that depending on light parameters and type of cell, pre‐exposure to LLLT can modulate the cytotoxic response of normal and cancer cells to γ radiation . Pre‐clinical and clinical trials report that LLLT following IR is able to reduce cell apoptosis and stimulate revascularization of parotid glands and to improve thyroid and liver functions besides enhancing antioxidant levels and the number of platelets .…”

Section: Introductionmentioning

confidence: 99%

Exploring the effects of low‐level laser therapy on fibroblasts and tumor cells following gamma radiation exposure

Silva¹,

Cabral²,

Camargo³

et al. 2016

Journal of Biophotonics

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Ionizing radiation (IR) induces DNA damage and low-level laser therapy (LLLT) has been investigated to prevent or repair detrimental outcomes resulting from IR exposure. Few in vitro studies, however, explore the biological mechanisms underlying those LLLT benefits. Thus, in this work, fibroblasts and tumor cells are submitted to IR with doses of 2.5 Gy and 10 Gy. After twenty-four-h, the cells are exposed to LLLT with fluences of 30 J cm , 90 J cm , and 150 J cm . Cellular viability, cell cycle phases, cell proliferation index and senescence are evaluated on days 1 and 4 after LLLT irradiation. For fibroblasts, LLLT promotes - in a fluence-dependent manner - increments in cell viability and proliferation, while a reduction in the senescence was observed. Regarding tumor cells, no influences of LLLT on cell viability are noticed. Whereas LLLT enhances cell populations in S and G /M cell cycle phases for both cellular lines, a decrease in proliferation and increase in senescence was verified only for tumor cells. Putting together, the results suggest that fibroblasts and tumor cells present different responses to LLLT following exposure to gamma-radiation, and these promising results should stimulate further investigations. Senescence of tumor cells and fibroblasts on the 4 day after ionizing radiation (IR) and low-level laser therapy (LLLT) exposures. The number of senescent cells increased significantly for tumor cells (a) while for fibroblasts no increment was observed (b). The blue collor indicates senescence activity.

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Analysis of Radiomodulatory Effect of Low-Level Laser Irradiation by Clonogenic Survival Assay

Cited by 19 publications

References 47 publications

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

Review on the Cellular Mechanisms of Low-Level Laser Therapy Use in Oncology

A Comparative Study of 660 nm Low-Level Laser and Light Emitted Diode in Proliferative Effects of Fibroblast Cells

Exploring the effects of low‐level laser therapy on fibroblasts and tumor cells following gamma radiation exposure

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