Analysis of radioprotection and antimutagenic effects of Ilex paraguariensis infusion and its component rutin

Abstract

DNA repair pathways, cell cycle checkpoints, and redox protection systems are essential factors for securing genomic stability. The aim of the present study was to analyze the effect of Ilex paraguariensis (Ip) infusion and one of its polyphenolic components rutin on cellular and molecular damage induced by ionizing radiation. Ip is a beverage drank by most inhabitants of Argentina, Paraguay, Southern Brazil, and Uruguay. The yeast Saccharomyces cerevisiae (SC7Klys 2-3) was used as the eukaryotic model. Exponentially growing cells were exposed to gamma rays (γ) in the presence or absence of Ip or rutin. The concentrations used simulated those found in the habitual infusion. Surviving fractions, mutation frequency, and DNA double-strand breaks (DSB) were determined after treatments. A significant increase in surviving fractions after gamma irradiation was observed following combined exposure to γ+R, or γ+Ip. Upon these concomitant treatments, mutation and DSB frequency decreased significantly. In the mutant strain deficient in MEC1, a significant increase in γ sensitivity and a low effect of rutin on γ-induced chromosomal fragmentation was observed. Results were interpreted in the framework of a model of interaction between radiation-induced free radicals, DNA repair pathways, and checkpoint controls, where the DNA damage that induced activation of MEC1 nodal point of the network could be modulated by Ip components including rutin. Furthermore, ionizing radiation-induced redox cascades can be interrupted by rutin potential and other protectors contained in Ip.

Development of a preliminary sensory lexicon for mate tea

“…Numerous active components have been identified in Yerba Mate. Some of them are antioxidant (Boaventura et al, ; Mateos, Baeza, Sarriá, & Bravo, ), anti‐obesity (Alkhatib, ; Arçari, Santos, Gambero, & Ribeiro, ), antidiabetic (Kang et al, ), neuroprotective (Santos et al, ), vasodilatory (Pontilho et al, ), anti‐mutagenic (Bracesco et al, ), anti‐glycation effects, increasing of energy, antimicrobial (Yuan & Yuk, ), and anti‐inflammatory (Luz et al, ).…”

Efecto radioprotector del ácido tánico y vinos de la var Vitis vinífera L. cv Tannat en Saccharomyces cerevisiae.

“…A partir de los resultados obtenidos en curvas dosisefecto de rayos gamma (50, 100 y 200 Gy), realizadas en nuestro laboratorio y considerando resultados publicados con dosis menores que no mostraron diferencias significativas; se seleccionó la dosis de radiación gamma de 200 Gy para los tratamientos simples y combinados (Tabla 2, Figura 2) (29,30) . Sosa, V., Contreras, V., Blanc, L., Dellacassa, E., Carrau, F., Bracesco Tratamientos combinados (2 agentes): diez minutos previos a la irradiación se trataron las alícuotas de células en medio nutriente con: AT y vino Tannat a las concentraciones antes señaladas para luego someterlas a una dosis de radiación gamma de 200 Gy.…”

Ilex Paraguariensis as a Healthy Food Supplement for the Future World

“…On the other hand, there are some authors who claimed that polycyclic aromatic hydrocarbons (PAH) derived from the incomplete combustion of wood as contaminants present in the yerba mate leaf, are responsible for the association between the infusion and cancer, because of the presence of PAH as they are recognized carcinogenic agents [22]. However, further Subsequent investigations documented the non-transmission of PAH to the infusion and also a protective effect of the infusion against the damage produced by certain PAHs [23]. In 2016, IARC committee of experts and proposes the reclassification of many foods, including yerba mate infusion.…”