Analysis of RAPD and AFLP markers linked to resistance to Fusarium oxysporum f. sp. lactucae race 2 in lettuce (Lactuca sativa L.)

Aruga, Daisuke; Tsuchiya, Nobuaki; Matsumura, Hideo; Matsumoto, Etsuo; Hayashida, Nobuaki

doi:10.1007/s10681-012-0665-5

Cited by 18 publications

(15 citation statements)

References 22 publications

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“…Many DNA-based marker systems, such as random amplified polymorphic DNA (RAPD), simple sequence repeat, amplified fragment length polymorphism, sequence characterized amplified region, and sequence-related amplified polymorphism have been used in this discipline (Palombi and Damiano, 2002;Lee et al, 2004;Fang et al, 2006;Comlekcioglu et al, 2010;Aruga et al, 2012). Among these, RAPD has proven to be a particularly useful means for cultivar analysis and genetic diversity detection (Williams et al, 1990) because it has the advantage of speed and simplicity.…”

Section: Introductionmentioning

confidence: 99%

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Cao

Zhou

et al. 2015

Genet. Mol. Res.

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ABSTRACT. Cultivar identification diagrams (CIDs) provide a rapid and efficient approach for identifying cultivars based on random amplified polymorphic DNA (RAPD) markers. In this paper, 64 tomato cultivars were identified using a CID. Using RAPD profiles, clustering analysis was performed to analyze genetic diversity. The results showed that 8 RAPD primers could completely separate the 64 cultivars according to the obtained polymorphic bands; a CID of the 64 tomato cultivars was then constructed. As verification of the CID validity, 8 randomly selected cultivars were investigated and proven to be well distinguished. In addition, 33 DNA bands were obtained, 20 (60.6%) of which were polymorphic. Genetic distances were calculated with a range of 0.032 to 1.402. Clustering analysis showed that the 64 tomato cultivars were divided into 4 groups with a similarity coefficient of 0.40. Using this novel strategy, with the same RAPD data, both CID and clustering analysis can simultaneously determine tomato cultivars and their genetic diversity.

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Section: Introductionmentioning

confidence: 99%

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Cao

Zhou

et al. 2015

Genet. Mol. Res.

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“…At least five race‐specific R ‐genes ( Dm5/8 , Dm10 , Dm17 , Dm43 , Dm45 ) for resistance to downy mildew (caused by Bremia lactucae ) are located on LG 1 (McHale, ; McHale et al ., ; Michelmore, ) together with genes for resistance to Lettuce mosaic virus ( Mo‐2 ; McHale et al ., ), Turnip mosaic virus ( Tu ; Robbins et al ., ), root downy mildew ( plr ; Kesseli et al ., ) and fusarium wilt ( FUS1 ; Michelmore, ). LG 2 harbours a large cluster of R genes for resistance to downy mildew ( Dm1 , Dm2 , Dm3 , Dm6 , Dm14 , Dm15 , Dm16 , Dm18 ; McHale, ; McHale et al ., ; Michelmore, ) and resistance genes against lettuce dieback ( Tvr1 ; Simko et al ., ), fusarium wilt ( FUS2 , RRD2 ; Michelmore, ; Aruga et al ., ) and anthracnose ( ANT2 ; McHale et al ., ). On LG 7 the only known major resistance gene is the FUS3 gene conferring resistance to fusarium wilt (Michelmore, ).…”

Section: Discussionmentioning

confidence: 99%

Evaluation and QTL mapping of resistance to powdery mildew in lettuce

et al. 2013

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Lettuce (Lactuca sativa) is the major leafy vegetable that is susceptible to powdery mildew disease under greenhouse and field conditions. Quantitative trait loci (QTLs) for resistance to powdery mildew under greenhouse conditions were mapped in an interspecific population derived from a cross between susceptible L. sativa cultivar Salinas and the highly susceptible L. serriola accession UC96US23. Four significant QTLs were detected on linkage groups LG 1 (pm-1.1), LG 2 (pm-2.1 and pm-2.2) and LG 7 (pm-7.1), each explaining between 35 to 42% of the phenotypic variation. The four QTLs are not located in the documented hotspots of lettuce resistance genes. Alleles for the disease resistance at the four QTLs originated from both parents (two from each), demonstrating that even highly susceptible accessions may provide alleles for resistance to powdery mildew. These QTLs appeared to operate during limited periods of time. Results of the field trials with F 2:3 and F 3:4 families derived from a Soraya (moderately resistant) 9 Salinas cross demonstrated effective transfer of resistance to powdery mildew in this material. An integrated rating approach was used to estimate relative levels of resistance in 80 cultivars and accessions tested in a total of 23 field and greenhouse experiments. Generally, very low resistance was observed in crisphead-type lettuces, while the highest relative resistance was recorded in leaf and butterhead types. Comparison of two disease assessment methods (percentage rating and the area under the disease progress steps, AUDPS) for detection of QTLs shows that the two approaches complement each other.

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“…“Eruption” x “Reine des Glaces” and “Delsay” x “Reine des Glaces” RIL populations Sandoya et al, 2019 Root rot QTL for race 2 major resistance locus L. sativa cvs. “VP1013” x “Patriot” F 2 population Aruga et al, 2012 Lettuce aphid Identified significant marker-trait associations for aphid resistance Diverse panel of wild and cultivated lettuce accessions, representing all major leaf types and of worldwide origin Walley et al, 2017 Post-harvest Shelf life Identification of multiple QTL for shelf life and association physiological, biochemical and biophysical traits, trait data collected in two field trials in two geographic locations L. sativa cv. Salinas and L. serriola acc.…”

Section: Targeting Plant Genetics For Improving the Post-harvest Qualmentioning

confidence: 99%

Innovative breeding technologies in lettuce for improved post-harvest quality

Damerum

Chapman

Taylor

2020

Postharvest Biology and Technology

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Highlights Delivering fresh, safe, high quality and long shelf life lettuce is a key challenge. Poor shelf life leads to major crop waste, largely overlooked in breeding programmes. Research has identified genetic variation for post-harvest quality. We outline multiomic datasets, genetic and genomic resources for lettuce breeding.

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Analysis of RAPD and AFLP markers linked to resistance to Fusarium oxysporum f. sp. lactucae race 2 in lettuce (Lactuca sativa L.)

Cited by 18 publications

References 22 publications

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

A novel random amplified polymorphic DNA-based strategy for genetic diversity analysis and identification of tomatoes

Evaluation and QTL mapping of resistance to powdery mildew in lettuce

Innovative breeding technologies in lettuce for improved post-harvest quality

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