Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity

Vorobieva, E.; Meringova, L. F.; Leontieva, Galina; Grabovskaya, K. B.; Suvorov, Alexander

doi:10.1007/bf02931468

Cited by 11 publications

(5 citation statements)

References 19 publications

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“…6 About 30% of GBS possess the bac gene which can be predominantly found in the strains of serotypes I and II. 7 The ScaAB protein is the major surface lipoprotein similar to the lipoprotein receptor-associated antigen I (LraI) family.. 8,9 This protein is presented on the surface of gram-positive pathogens and closely related to PsaA protein of S pneumoniae which is considered a protein-based vaccine candidate against pathogenic pneumococci which are the most common cause of community-acquired pneumonia. 10 The ScpB1 protein represents the portion of C5a peptidase, which is contained among 100% of strains of group A and B streptococci, thus providing a universal target for vaccines against pathogenic streptococci.…”

Section: Introductionmentioning

confidence: 99%

Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

et al. 2017

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We investigate the protective effect of combined vaccination based on live attenuated influenza vaccine (LAIV) and group B streptococcus (GBS) recombinant polypeptides against potential pandemic H7N9 influenza infection followed by GBS burden. Mice were intranasally immunized using 107 50% egg infectious dose (EID50) of H7N3 LAIV, the mix of the 4 GBS peptides (group B streptococcus vaccine [GBSV]), or combined LAIV + GBSV vaccine. The LAIV raised serum hemagglutination-inhibition antibodies against H7N9 in higher titers than against H7N3. Combined vaccination provided advantageous protection against infections with A/Shanghai/2/2013(H7N9)CDC-RG influenza and serotype II GBS. Combined vaccine significantly improved bacterial clearance from the lungs after infection compared with other vaccine groups. The smallest lung lesions due to combined LAIV + GBSV vaccination were associated with a prevalence of lung interferon-γ messenger RNA expression. Thus, combined viral and bacterial intranasal immunization using H7N3 LAIV and recombinant bacterial polypeptides induced balanced adaptive immune response, providing protection against potential pandemic influenza H7N9 and bacterial complications.

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Section: Introductionmentioning

confidence: 99%

Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

et al. 2017

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“…Indeed, Tettelin et al (2002) did not detect expression of SAG1533 (MtsA) on the GBS surface using FACS analysis. However, it is worth noting that antibodies against MtsA were detected in sera of mice following infection with GBS (Vorobieva et al 2005). Antibodies to homologues of MtsA were also found in convalescent sera of humans with GAS and pneumococcal infections (Lei et al 2004;Rapola et al 2000) and in mice experimentally infected with GAS (Lei et al 2004).…”

Section: Discussionmentioning

confidence: 97%

Expression of the MtsA lipoprotein of Streptococcus agalactiae A909 is regulated by manganese and iron

Bray

Sutcliffe

Harrington

2008

Antonie van Leeuwenhoek

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Metal ion acquisition and homeostasis are essential for bacterial survival, growth and physiology. A family of metal ion, ABC-type import systems have been identified in Gram-positive bacteria, in which the solute-binding proteins are predicted to be membrane-anchored lipoproteins. The prediction that the MtsA protein of Streptococcus agalactiae A909 is a lipoprotein was confirmed. The expression of MtsA was co-ordinately regulated by the presence of both manganese and ferrous ions suggesting that MtsA may be involved in the uptake of both these ions. MtsA was shown to be expressed at levels of ferrous ions known to be present in amniotic fluid, a growth medium for S. agalactiae during neonatal infection.

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“…pneumoniae infection [17]. Recently, Seon et al, 2017 have shown that attenuated pneumococcus pep27 mutant which protect mice against secondary pneumococcal challenge after influenza virus infection increased antibody titers against PspA protein but not type 2 capsular polysaccharide These data suggest that although pneumococcus is surrounded by a powerful polysaccharide capsule, at the stages of its reproduction, the internal proteins become available for molecular interaction and the antibodies forming against these proteins are protective [18].…”

Section: Discussionmentioning

confidence: 99%

Mucosal vaccine based on attenuated influenza virus and the group B Streptococcus recombinant peptides protected mice from influenza and S. pneumoniae infections

et al. 2019

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Although many influenza-related deaths are attributable to secondary bacterial infection with S . pneumoniae , vaccines that simultaneously protect against influenza and pneumococcal infection are currently not developed. The aim of our study was to evaluate the possibility to prevent post-influenza pneumococcal infection using an associated vaccine based on live influenza vaccine (LAIV) combined with recombinant polypeptides derived from superficial factors of Group B streptococcus (GBS) determining pathogenicity. We demonstrated in a model of post-influenza pneumococcal pneumonia that intranasal pneumococcal super-infection seriously complicated the course of A/Shanghai/2/2013(H7N9) CDC-RG virus infection in mice. Associated immunization using LAIV and GBS vaccine (GBSV) prevented post-influenza pneumococcal pneumonia better than mono-LAIV or GBSV immunization. At the same time, parenteral pneumococcal post-influenza infection of immune mice was more severe in the groups immunized using recombinant GBS peptides which can be explained by antibody-dependent enhancement of infection. In this case, the introduction of blockers of histamine receptors type 1 and 2 reduced the burden of secondary pneumococcal infection.

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Analysis of recombinant group B streptococcal protein ScaAB and evaluation of its immunogenicity

Cited by 11 publications

References 19 publications

Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

Prevention of Influenza A(H7N9) and Bacterial Infections in Mice Using Intranasal Immunization With Live Influenza Vaccine and the Group B Streptococcus Recombinant Polypeptides

Expression of the MtsA lipoprotein of Streptococcus agalactiae A909 is regulated by manganese and iron

Mucosal vaccine based on attenuated influenza virus and the group B Streptococcus recombinant peptides protected mice from influenza and S. pneumoniae infections

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