Analysis of repair of abasic sites in early onset breast cancer patients

Rossi, Ottavio; Carrozzino, Fabio; Cappelli, Enrico; Carli, Franca; Fròsina, Guido

doi:10.1002/(sici)1097-0215(20000101)85:1<21::aid-ijc4>3.3.co;2-k

Cited by 5 publications

(7 citation statements)

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“…The vital role of APE1 in mammalian systems is also supported by findings demonstrating either an altered expression pattern of APE1 [105,122] or mutations in APE1 [123–125] being associated with pathological conditions including cancer and neurodegenerative disorders [126]. However, such disease conditions are not always correlated with impaired DNA repair function of APE1, thus suggesting that other cellular functions of APE1 may be more important [127,128]. APE1 was also recently shown to interact with ribosomal RNA and NPM1, which is a nuclear protein involved in a variety of cellular processes including ribosome biogenesis [129].…”

Section: Ape1mentioning

confidence: 95%

Endoribonucleases – enzymes gaining spotlight in mRNA metabolism

Barnes

Lee

2010

The FEBS Journal

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The efficient turnover of messenger RNA represents an important mechanism that allows the cell to control gene expression. Until recently, the mechanism of mRNA decay was mainly attributed to exonucleases, comprising enzymes that degrade RNAs from the ends of the molecules. This article summarizes the endoribonucleases, comprising enzymes that cleave RNA molecules internally, which were identified in more recent years in eukaryotic mRNA metabolism. Endoribonucleases have received little attention in the past, based on the difficulty in their identification and a lack of understanding of their physiological significance. This review aims to compare the similarities and differences among this group of enzymes, as well as their known cellular functions. Despite the many differences in protein structure, and thus difficulties in identifying them based on amino acid sequence, most endoribonucleases possess essential cellular functions and have been shown to play an important role in mRNA turnover.

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Section: Ape1mentioning

confidence: 95%

Endoribonucleases – enzymes gaining spotlight in mRNA metabolism

Barnes

Lee

2010

The FEBS Journal

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“…These abnormal sites can be formed from spontaneous depurination, deamination, ionizing radiation or from repair mechanisms in plants and animals [5–7]. The abasic sites can potentially cause carcinogenesis, in addition to cellular death [8]. Since the DNA damage sustained from one DNA molecule to another is different, a sensitive and reliable method to detect AP sites on individual DNA molecules is fundamentally essential in the field of genetic studies.…”

Section: Introductionmentioning

confidence: 99%

Near‐field optical imaging of abasic sites on a single DNA molecule

Kim¹,

Muramatsu²,

Lee³

et al. 2003

FEBS Letters

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Scanning near-¢eld optical microscopy (SNOM) imaging was performed to allow for the direct visualization of damaged sites on individual DNA molecules to a scale of a few tens of nanometers. Fluorescence in situ hybridization on extended DNA molecules was modi¢ed to detect a single abasic site. Abasic sites were speci¢cally labelled with a biotinlylated aldehyde-reactive probe and £uorochrome-conjugated streptavidin. By optimizing the performance of the SNOM technique, we could obtain high contrast near-¢eld optical images that enabled high-resolution near-¢eld £uorescence imaging using optical ¢ber probes with small aperture sizes. High-resolution near-¢eld £uorescence imaging demonstrated that two abasic sites within a distance of 120 nm are clearly obtainable, something which is not possible using conventional £uorescence in situ hybridization combined with far-¢eld £uorescence microscopy.

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“…Repair of AP sites in PBL was analyzed by an incision assay on randomly depurinated plasmid DNA [16,17]. Figure 1 shows the AP site incision activity in PBL from controls (Con) and cases (Cas).…”

Section: Resultsmentioning

confidence: 99%

“…The assay for incision of AP sites in PBL has been described [15][16][17]. Briefly, lymphocytes from 5 ml blood were isolated by Ficoll (ICN, Milano, Italy) and resuspended at a cell density of 10 7 cells/ml in lysis buffer (0.07 M Hepes.…”

Section: Ap Sites Incision Assaymentioning

confidence: 99%

Incision of AP sites in lung cancer patients: a pilot study

Rossi

Filiberti

Neri

et al. 2002

Teratog Carcinog Mutagen

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DNA base excision repair (BER) removes frequent DNA lesions of either endogenous or exogenous origin. Some indications point to BER defects in lung cancer patients. We have investigated the ability of ten lung cancer patients to repair natural AP sites, the most frequent genetic lesion, using an in vitro assay in which peripheral blood lymphocytes (PBL) extracts incise randomly depurinated plasmid DNA. The median value of repair activity in patients was lower than that of matched controls but the difference did not reach significance. Unlike other BER enzymatic steps, marked defects in incision of AP sites may not be associated with lung carcinogenesis.

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Analysis of repair of abasic sites in early onset breast cancer patients

Cited by 5 publications

References 0 publications

Endoribonucleases – enzymes gaining spotlight in mRNA metabolism

Endoribonucleases – enzymes gaining spotlight in mRNA metabolism

Near‐field optical imaging of abasic sites on a single DNA molecule

Incision of AP sites in lung cancer patients: a pilot study

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