Analysis of seasonal and annual field-evolved insecticide resistance in populations of Thrips hawaiiensis in banana orchards

Germinara

et al. 2020

Insects

Self Cite

Thrips hawaiiensis is a common thrips pest of various plant flowers with host preference. Plant volatiles provide important information for host-searching in insects. We examined the behavioral responses of T. hawaiiensis adults to the floral volatiles of Gardenia jasminoides Ellis, Gerbera jamesonii Bolus, Paeonia lactiflora Pallas, and Rosa chinensis Jacq. in a Y-tube olfactometer. T. hawaiiensis adults showed significantly different preferences to these four-flower plants, with the ranking of G. jasminoides > G. jamesonii > P. lactiflora ≥ R. chinensis. Further, 29 components were identified in the volatile profiles of G. jasminoides, and (Z)-3-hexenyl tiglate (14.38 %), linalool (27.45 %), and (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (24.67 %) were the most abundant. Six-arm olfactometer bioassays showed that T. hawaiiensis had significant positive responses to (Z)-3-hexenyl tiglate, linalool, and (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene tested at various concentrations, with the most attractive ones being 10−3 μL/μL, 10−2 μL/μL and 100 μg/μL for each compound, respectively. In pairing of these three compounds at their optimal concentrations, T. hawaiiensis showed the preference ranking of (Z)-3-hexenyl tiglate > linalool > (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene. Large numbers of T. hawaiiensis have been observed on G. jasminoides flowers in the field, which might be caused by the high attraction of this pest to G. jasminoides floral volatiles shown in the present study. Our findings shed light on the olfactory cues routing host plant searching behavior in T. hawaiiensis, providing important information on how T. hawaiiensis targets particular host plants. The high attractiveness of the main compounds (e.g., linalool, (E3,E7)-4,8,12-trimethyltrideca-1,3,7,11-tetraene, particular (Z)-3-hexenyl tiglate) identified from volatiles of G. jasminoides flowers may be exploited further to develop novel monitoring and control tools (e.g., lure and kill strategies) against this flower-inhabiting thrips pest.

Section: Introductionmentioning

confidence: 99%

Behavioral Responses of Thrips hawaiiensis (Thysanoptera: Thripidae) to Volatile Compounds Identified from Gardenia jasminoides Ellis (Gentianales: Rubiaceae)

Cao

Germinara

et al. 2020

Insects

Self Cite

Kansai Byochugai Kenkyukaiho

“…2). This thrips, however, developed resistance to pesticides in banana plantations in south China (Fu et al, 2018(Fu et al, , 2019. Pesticides have been applied against thrips by most growers in several areas, with more than 20 applications per banana-growing cycle (Fu et al, 2018).…”

Section: Perspectivementioning

confidence: 99%

A Review of Ecological Factors Associated with Wild Host Plants as Refuges for Suppressing Pesticide Resistance in Agricultural Arthropod Pests

Morishita

2021

Arthropod pests continue to occur throughout the year by inter-plant movement and dispersal between cultivated host plants that are applied with pesticides and wild host plants that serve as refuges in the area of cultivation. This paper reviews the relationship between the host range and status of pesticide resistance in agricultural arthropod pests in Japan. The decisive factors for determining the development of pesticide resistance were concluded with attention paid to wild host plants as refuges. The arthropod pests that developed pesticide resistance were as follows: (1) monophagous species infesting cultivated crops treated with pesticides, (2) polyphagous species with a host range restricted to cultivated plants lacking wild host plants, and (3) certain populations of polyphagous species develop resistance in habitats such as tea fields and greenhouses with yearround cultivation because they are relatively isolated from the surrounding populations that do not develop resistance due to the abundance of refuges. In all cases, pest populations inhabit environments where they are restricted to mate with the susceptible populations from refuges of wild host plants.

“…Resistance to insecticides in insect pests is often regulated by physiological changes that are due to detoxification enzymes 13 . Many studies demonstrated the detoxification enzymes (cytochrome P450s, glutathione S‐transferases, and carboxylesterase) mediate resistance in various insects, including H. armigera , 9,14,15 Spodoptera littoralis , 16 Cydia pomonella , 17 Thrips hawaiiensis , 18 Spodoptera exigua , 19 and Spodoptera frugiperda 20 . In West Africa, deltamethrin resistance in H. armigera were suppressible by piperonyl butoxide (PBO) and trichlorophenyl propynyl ether (TCPPE), thus indicating a major role of P450 enzyme(s) in H. armigera resistance 15 .…”

Section: Introductionmentioning

confidence: 99%

Field‐evolved resistance to 11 insecticides and the mechanisms involved in Helicoverpa armigera (Lepidoptera: Noctuidae)

Rui

Pest Management Science

et al. 2021

BACKGROUND To understand the ongoing resistance of cotton bollworm, Helicoverpa armigera, the sensitivity of five field populations to commonly used insecticides, indoxacarb, abamectin, methoxyfenozide, chlorfenapyr, chlorantraniliprole, spinetoram, lambda‐cyhalothrin, carbosulfan, metaflumizone, chlorpyrifos, and flufenoxuron, were evaluated. Furthermore, the biochemical and molecular mechanisms of field‐evolved resistance in H. armigera were also investigated. RESULTS Five field populations of H. armigera showed moderate resistance to indoxacarb, chlorantraniliprole, metaflumizone, methoxyfenozide, carbosulfan and lambda‐cyhalothrin. The resistance ratio (RR) of indoxacarb was significantly correlated with glutathione‐S‐transferases (GSTs) activity (r = 0.913, P = 0.011). Methoxyfenozide RR was largely correlated with cytochrome P450s activity (r = 0.860, P = 0.028). Besides, six cytochrome P450s genes of CYP4L5 in AQP, CYP6B7 and CYP9A14 in HDP and BDP, CYP9A17V2 in HDP and YSP, CYP332A1 in HDP, LFP, AQP and YSP, CYP337B1 in YSP, and two GSTs genes of GSTd1 and GSTs1 in HDP were overexpressed (>5‐fold). Moreover, indoxacarb RR was positively correlated with the overexpression of GSTs1, GSTd1 and CYP9A14 genes (r = 0.880, 0.98 and 0.86, P = 0.021, 0.001 and 0.028, respectively). The transcript of CYP9A17V2 and CYP337B1 were found to be correlated with metaflumizone RR (r = 0.950, P = 0.004) and carbosulfan RR (r = 0.850, P = 0.033), respectively. CONCLUSION H. armigera can be effectively controlled using abamectin, chlorfenapyr, chlorpyrifos and spinetoram in Hebei and Shandong provinces. The present study demonstrated that the relative expression level of GSTs1, GSTd1, CYP9A14, CYP9A17V2 and CYP337B1 genes were significantly correlated with the resistance ratio to indoxacarb, metaflumizone and carbosulfan in field H. armigera.