Analysis of Seawaters for the Recovery of Culturable <i>Vibrio parahaemolyticus</i> and Some Other Vibrios

Alam, M.J.; Tomochika, Ken Ichi; Miyoshi, Shin‐ichi; Shinoda, Sumió

doi:10.1111/j.1348-0421.2001.tb02636.x

Cited by 18 publications

(10 citation statements)

References 32 publications

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“…Also, the concentration of V. parahaemolyticus varied greatly between the methods ( Table 3). Selective and enrichment media are known to give decreased sensitivity of detection for natural populations for a variety of reasons, including poor or no growth on selective media [1,15], as well as competition with other bacteria comprising the microbial community of the natural environment when enrichment broth is employed [23]. This may explain why MPN-culture estimation of total V. parahaemolyticus may be signi¢cantly less than for MPN-PCR estimation using toxR primers.…”

Section: Resultsmentioning

confidence: 99%

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Alam

2002

FEMS Microbiology Letters

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Seawater and organic material (live and/or dead matter deposited on any substratum submersed in seawater) were collected during the cool weather season from a coast of the Seto-Inland Sea, Japan, and analyzed to determine Vibrio parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by the polymerase chain reaction (PCR), using isolated DNA from enrichment culture of the samples. About 95% of the samples were positive for V. parahaemolyticus (with densities of 3 to s 1400 cells per 100 ml water or 10 g organic samples) by the most-probable-number (MPN)-PCR technique with species-specific toxR primers, but only 40% were positive by the conventional MPN-culture technique (with densities ranging from 3 to 240 cells per 100 ml water or 10 g organics). Furthermore, the tdh and trh genes were positive in 55% and 20% of samples, respectively, by the MPN-PCR technique. No tdh and trh gene-positive strains were isolated by the conventional MPN-culture procedure. The difference in detection between the MPN-culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors. ß

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Section: Resultsmentioning

confidence: 99%

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Alam

2002

FEMS Microbiology Letters

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“…Also, the concentration of V. parahaemolyticus measured varied greatly between the methods (Table 3). Selective and enrichment media are known to give decreased sensitivity of detection for natural populations for a variety of reasons, including poor or no growth on selective media (Kaysner et al ., 1989; Alam et al ., 2001), as well as competition with other bacteria comprising the microbial community of the natural environment when enrichment broth is used (Tamplin et al ., 1991). This may explain why MPN culture estimation of total V. parahaemolyticus may be significantly less than for MPN‐PCR estimation using toxR primers.…”

Section: List Of Primers Target Genes and Amplicon Sizesmentioning

confidence: 99%

Studies on pathogenic Vibrio parahaemolyticus during a warm weather season in the Seto Inland Sea, Japan

Alam

Miyoshi

Shinoda

2003

Environmental Microbiology

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Vibrio parahaemolyticus is a potentially pathogenic bacterium, occurring naturally in estuarine and marine environments throughout the world. The incidence of this organism in an aquatic environment depends upon many ecofactors. Sea water and organic material were collected during the warm weather season from a coast of the Seto Inland Sea, Japan, and analysed to determine V. parahaemolyticus densities and the occurrence of pathogenic strains, defined as those possessing tdh and/or trh genes by polymerase chain reaction (PCR), using isolated DNA from enrichment culture of the samples. About 99% of samples were positive for V. parahaemolyticus with densities of 3 to >1400 cells per 100 ml of water or 10 g of organic samples by the most-probable-number (MPN)-PCR technique, but only 76.6% were positive by the conventional MPN culture technique, with densities ranging from 3 to >1400 cells per 100 ml of water or 10 g of organics. Furthermore, the tdh and trh genes were positive in 41.5% and 8.5% of samples, respectively, by the MPN-PCR technique. No tdh and trh gene-positive strains were isolated by the conventional MPN culture procedure. The difference in detection between the MPN culture and the MPN-PCR techniques appeared to be significant and may be attributed to different detection sensitivities and other factors.

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“…The availability of nutrients may lead to an excessive production of biomass, with bacterial proliferation being stimulated by the extracellular products of the phytoplankton, which may favour the development of Vibrio spp. (Alam, Tomochika, Miyoshi & Shinoda ). This would be expected in the BS, then, although the results of the present study indicate that, even with the increased availability of nutrients in this system, the growth of Vibrio spp.…”

Section: Resultsmentioning

confidence: 95%

Microbiological quality ofLitopenaeus vannameiculture using conventional and biofloc systems

et al. 2015

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Shrimp farming is a fast-expanding activity that has supported the growth in the supply of these crustaceans to consumers around the world. However, the end product is vulnerable to contamination at all stages of the process, including the rearing tanks, where current practices prioritize to raise stocking densities and the minimization of water renewal. It is thus important to evaluate the potential of these systems for the proliferation of undesirable microorganisms, which may render the product unfit for human consumption. In the present study, the presence of coagulase-positive Staphylococcus, Salmonella spp., Vibrio spp., and total and thermotolerant coliforms was verified in biofloc tank and conventional pond systems used for the rearing of Litopenaeus vannamei in Pernambuco, Brazil, and the results were compared with the legislation regulating the marketing of fresh shrimp. Samples were collected from two biofloc tanks with a density of 375 shrimp m À2 , and two conventional ponds with 12 shrimp m À2 . None of the samples tested positive for either Salmonella spp. or coagulase-positive Staphylococcus, which is consistent with the legislation. While no standards are defined legally for Vibrio spp. or coliforms, very low concentrations were recorded in both systems, in comparison with other studies. While some variation in bacterial contamination was observed over the rearing process, the end product of both systems was fit for human consumption. The results of the study indicate that, while the water is not renewed in the biofloc system, the development of undesirable microorganisms can be controlled, with no adverse effects for the end product.

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Analysis of Seawaters for the Recovery of Culturable Vibrio parahaemolyticus and Some Other Vibrios

Cited by 18 publications

References 32 publications

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Environmental investigation of potentially pathogenic Vibrio parahaemolyticus in the Seto-Inland Sea, Japan

Studies on pathogenic Vibrio parahaemolyticus during a warm weather season in the Seto Inland Sea, Japan

Microbiological quality ofLitopenaeus vannameiculture using conventional and biofloc systems

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