Analysis of selected veterinary antibiotics in fish by micellar liquid chromatography with fluorescence detection and validation in accordance with regulation 2002/657/EC

Rambla-Alegre, Maria; Péris-Vicente, Juan; Esteve‐Romero, Josep; Carda‐Broch, Samuel

doi:10.1016/j.foodchem.2010.05.119

Cited by 65 publications

(53 citation statements)

References 22 publications

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“…Moreover, micellar mobile phases are non-flammable, less toxic, more environmentally friendly, and relatively inexpensive than those used in hydroorganic-HPLC. 34 Micellar liquid chromatography has been previously proposed to detect chemical pollutants in wastewater, 35 and the pesticide carbaryl 36 in environmental water.…”

Section: Introductionmentioning

confidence: 99%

Analysis of thiabendazole, 4-tert-octylphenol and chlorpyrifos in waste and sewage water by direct injection – micellar liquid chromatography

Romero-Cano

Kassuha

Péris-Vicente

et al. 2015

Analyst

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A micellar liquid chromatographic method has been developed for the simultaneous quantification of the pesticides thiabendazole and chlorpyrifos, as well as an alkylphenol, which is included in pesticide formulations, i.e., 4-tert-octylphenol, in water. A sample was filtered and directly injected, avoiding large extraction steps using toxic solvents, thus expediting the experimental procedure. The contaminants were eluted without interferences in <17 min, using a mobile phase of 0.15 M sodium dodecyl sulfate -6% 1-pentanol buffered at pH 3, running through a C18 column at 1 mL min −1 under the isocratic mode. This optimal mobile phase was selected using a statistical approach, which considers the retention factor, efficiency and peak shape of the analytes measured in only a few mobile phases. The detection was carried out by measuring absorbance at 220 nm. The method was successfully validated in terms of specificity, calibration range (0.5-10 mg L −1 ), linearity (r 2 > 0.994), limit of detection and quantification (0.2-0.3; and 0.5-0.8 mg L −1 , respectively), intra-and interday accuracy (95.2-102.9%), precision (<8.3%), and ruggedness (<9.3%). The stability in storage conditions (at least 14 days) was studied. The method was safe, inexpensive, produced little pollutant and has a short analysis time, thus it is useful for the routine analysis of samples. Finally, the method was applied to analyse wastewater from the fruit-processing industry, wastewater treatment plants, and in sewage water belonging to the Castelló area (Spain). The results were similar to those obtained by an already reliable method.

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Section: Introductionmentioning

confidence: 99%

Analysis of thiabendazole, 4-tert-octylphenol and chlorpyrifos in waste and sewage water by direct injection – micellar liquid chromatography

Romero-Cano

Kassuha

Péris-Vicente

et al. 2015

Analyst

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“…8,16 After a simple dilution and filtration, samples are able to be directly injected, expediting the experimental procedure. 17 Our previously developed analytical method 6 can be adapted by the modification of the mobile phases in order to analyze three determined antiretrovirals. This way, this modified methodology will keep the benefits of micellar liquid chromatography, including the use of a low amount of pollutant chemicals.…”

Section: 9mentioning

confidence: 99%

Monitoring of HAART regime antiretrovirals in serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Casas-Breva¹,

Péris-Vicente

Rambla-Alegre

et al. 2012

Analyst

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A methodology based on micellar liquid chromatography to monitor five antiretroviral drugs (lamivudine, stavudine, tenofovir, zidovudine and efavirenz) was proposed. Antiretrovirals were studied in sets of three, corresponding to each highly active antiretroviral therapy (HAART) regime, prescribed to acquired immunodeficiency syndrome (AIDS)-infected patients. Four aqueous micellar mobile phases buffered at pH 7 were optimized to separate these compounds, using sodium dodecyl sulfate as the tensioactive, and 1-propanol or 1-pentanol as the organic modifier. The composition of each mobile phase was optimized for each antiretroviral. The common separation conditions were: C18 apolar column (125 Â 4.6 mm, 5 mm particle size), UV detection set at 214 nm, and mobile phase running at 1 mL min À1 without controlling the temperature. The finally suggested method was validated for five analysed antiretroviral drugs following the US Food and Drug Administration guidelines in terms of: linearity between 0.5 and 50 ppm (r 2 > 0.9995), sensitivity (LOD lower than 0.25 ppm), intra-and inter-day precision (<7.1 and <5.2%, respectively) and accuracy (recovery 88.5-105.3% and 93.5-101.3%, respectively), as well as robustness (<6.5%). The proposed method was used to monitor the level of antiretrovirals in the serum of AIDS patients. The suggested methodology was found to be useful in the routine analysis of antiretrovirals in serum samples.

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“…Sodium dodecyl sulphate (SDS) is an anionic surfactant and usually is used as a micellar media because it is easily dissolved in water and could be removed from the chromatographic system. MHPLC methods based on the use of SDS and a small amount of organic modifier have been applied as a novel technology of separation in different disciplines such as food science [19,20], pharmacy [21,22], and environmental science [23] since it was put forward by Armstrong [24].…”

Section: Introductionmentioning

confidence: 99%

Development micellar HPLC method for simultaneous determination of ephedrine, pseudoephedrine, and methylephedrine in Ephedra Herb and traditional Chinese medicinal preparations

Dong

et al. 2015

Acta Chromatographica

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Summary.A micellar high-performance liquid chromatography (HPLC) method has been described for simultaneous determination of ephedrine, pseudoephedrine, and methylephedrine in Ephedra Herb and two traditional Chinese preparations. The separation and determination of ephedrine, pseudoephedrine, and methylephedrine were performed using a mobile phase containing 1.75 × 10 −1 mol·L −1 sodium dodecyl sulphate and 0.02 mol·L −1 potassium hydrogen phosphate with 10% (v/v) methanol at pH 3.0, running at 1.5 mL·min −1 by a Venusil XBP C18 (250 × 4.6 mm, 5 μm) column at 40 °C. The detected wavelength was set at 210 nm. The method was validated according to the International Conference on Harmonization of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines. The main analytical parameters were linearity (r > 0.9990), intra-and inter-day precisions (relative standard deviation [RSD %], 0.33-1.63, and RSD %, 1.26-2.20, respectively), limit of quantifications [LOQs], and limit of detections [LODs] (2.6 × 10 −4 and 7.8 × 10 −5 mg·mL −1 for ephedrine, 6.8 × 10 −4 and 2.0 × 10 −4 mg·mL −1 for pseudoephedrine, and 5.0 × 10 −4 and 1.5 × 10 −4 mg·mL −1 for methylephedrine). RSDs of recoveries were <5.5% in the three samples. Based on the optimized chromatographic conditions and the eluted orders, a model of separation mechanism for the analytes was established. The results indicated that the proposed method was an accurate, "green" and cheap method.

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Analysis of selected veterinary antibiotics in fish by micellar liquid chromatography with fluorescence detection and validation in accordance with regulation 2002/657/EC

Cited by 65 publications

References 22 publications

Analysis of thiabendazole, 4-tert-octylphenol and chlorpyrifos in waste and sewage water by direct injection – micellar liquid chromatography

Analysis of thiabendazole, 4-tert-octylphenol and chlorpyrifos in waste and sewage water by direct injection – micellar liquid chromatography

Monitoring of HAART regime antiretrovirals in serum of acquired immunodeficiency syndrome patients by micellar liquid chromatography

Development micellar HPLC method for simultaneous determination of ephedrine, pseudoephedrine, and methylephedrine in Ephedra Herb and traditional Chinese medicinal preparations

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