Analysis of seven STR human loci for paternity testing by microchip electrophoresis

Fraige, Karina; Travensolo, Regiane F.; Carrilho, Emanuel

doi:10.1590/s1516-89132013000200006

Cited by 8 publications

(9 citation statements)

References 8 publications

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“…Then, in the current study the extracted salivary DNA was used as a template for amplification using three autosomal human specific genes (D21S11, D18S51 and CSF1PO) with relatively small size (ranging from 214 to 323bp). These three loci were chosen as they are among the thirteen STR markers selected to form the core of FBI Laboratory's Combined DNA Index System (Fraige et al, 2013). Important points before starting -All reaction mixtures were done in an area separate from that used for DNA preparation or PCR product analysis.…”

Section: Scoring Resultsmentioning

confidence: 99%

“…Thirteen STR loci -used for paternity identification-were successfully amplified by PCR after DNA extraction from saliva and blood. The conditions for the PCR were optimized using the DNA from the saliva samples, and the same conditions were applied to the DNA extracted from blood yielding the same results, i.e., good quality and quantity of DNA extracted (Fraige et al, 2013). Chávez-Briones et al, (2015) recorded the second case in forensic literature reporting the analysis of DNA isolated from bite marks present on a victim s' body in order to identify the perpetrator of a crime.…”

Section: Discussionmentioning

confidence: 99%

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Rapid Stain Biomarker : A Novel Tool for the Forensic Detection of Saliva (Effect of Time and Surface on its Reliability )

Mahmoud

Zaki

2018

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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Saliva is one of the vital fluids secreted by the human body; it can be deposited on the human skin or other materials through biting, sucking, licking and kissing. Saliva stains encountered in forensic casework is an important source of DNA; so, it is considered an important evidence for personnel identification. The rapid Stain Identification of Human Saliva (RSID TM-Saliva) bioassay is designed to detect specifically the presence of human salivary -amylase enzyme. Test development is completed within 10 minutes and can detect as little as 1l of human saliva. The detection protocol can be completely integrated into the procedures for DNA extraction and analysis. Aim: Assess the efficacy of RSID TM-Saliva strip test for the detection of human saliva under some different variables (different surfaces & different time intervals).Evaluate the quality of DNA extracted from unpreserved saliva and the possibility of its usage as evidence in forensics. Methodology: Fifty two saliva samples were collected from four volunteers. Forty eight of them were deposited over surfaces of different nature (plastic bottles, glass cup and cigarette butts) at the same time. Four saliva samples were collected directly from the floor of the mouth of each participant by a cotton swab (positive control group). All samples were left to dry for 10 min at room temperature then extracted and analyzed according to the protocol designed for (RSID TM-Saliva). Collected samples were studied as three tested groups and as control groups. Saliva samples collected from each participant were categorized into four subgroups according to the time interval of saliva extraction [zero (on the spot), 10, 20, and 30 days].Positive samples for saliva identification were subjected to DNA extraction and typing; as three autosomal primers short tandem repeats (STRs) specific to human genomic DNA (D21S11, D18S51 and CSF1PO) were amplified by polymerase chain reaction (PCR); then subjected to separation and analysis of fragments size by agarose gel electrophoresis to determine their allelic size through comparison to the standard allelic 100 bp ladder. Results: Saliva could be identified in 100% of tested samples as well as for the positive control samples, which were subjected to successful DNA extraction followed by PCR amplification of the chosen three STRs. Results of agarose gel revealed that the sizes of these PCR products were in accordance with the 100 bp DNA ladder; validating the good quality and quantity of the extracted DNA Conclusion: the current study demonstrated that non-stored saliva deposited over different surfaces for up to 30 days presents an ideal source for DNA which may be used for forensic identification. The study also evidenced that the new RSID-saliva strip test is a fast, easy, sensitive and reliable method for saliva identification over different surfaces.

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Section: Scoring Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Rapid Stain Biomarker : A Novel Tool for the Forensic Detection of Saliva (Effect of Time and Surface on its Reliability )

Mahmoud

Zaki

2018

Ain Shams Journal of Forensic Medicine and Clinical Toxicology

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“…One drawback of this method is the potential for misidentification of multiple blood meals. However, using the Agilent Bioanalyzer [ 65 ] or MALDI-ToF [ 66 ] for genotyping could provide a cost-effective alternative to capillary electrophoresis-based systems.…”

Section: Discussionmentioning

confidence: 99%

Understanding mosquito host-choice behaviour: a new and low-cost method of identifying the sex of human hosts from mosquito blood meals

et al. 2021

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Background Mosquito-borne diseases are a global health problem, causing hundreds of thousands of deaths per year. Pathogens are transmitted by mosquitoes feeding on the blood of an infected host and then feeding on a new host. Monitoring mosquito host-choice behaviour can help in many aspects of vector-borne disease control. Currently, it is possible to determine the host species and an individual human host from the blood meal of a mosquito by using genotyping to match the blood profile of local inhabitants. Epidemiological models generally assume that mosquito biting behaviour is random; however, numerous studies have shown that certain characteristics, e.g. genetic makeup and skin microbiota, make some individuals more attractive to mosquitoes than others. Analysing blood meals and illuminating host-choice behaviour will help re-evaluate and optimise disease transmission models. Methods We describe a new blood meal assay that identifies the sex of the person that a mosquito has bitten. The amelogenin locus (AMEL), a sex marker located on both X and Y chromosomes, was amplified by polymerase chain reaction in DNA extracted from blood-fed Aedes aegypti and Anopheles coluzzii. Results AMEL could be successfully amplified up to 24 h after a blood meal in 100% of An. coluzzii and 96.6% of Ae. aegypti, revealing the sex of humans that were fed on by individual mosquitoes. Conclusions The method described here, developed using mosquitoes fed on volunteers, can be applied to field-caught mosquitoes to determine the host species and the biological sex of human hosts on which they have blood fed. Two important vector species were tested successfully in our laboratory experiments, demonstrating the potential of this technique to improve epidemiological models of vector-borne diseases. This viable and low-cost approach has the capacity to improve our understanding of vector-borne disease transmission, specifically gender differences in exposure and attractiveness to mosquitoes. The data gathered from field studies using our method can be used to shape new transmission models and aid in the implementation of more effective and targeted vector control strategies by enabling a better understanding of the drivers of vector-host interactions.

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“…10 Unavailability of child's parent information for comparison in the forensic DNA examination process is one of the problems that might occur in forensic DNA analysis. 1 Unlike DNA testing that uses thparent's DNA as comparisons, individual identification using siblings' DNA has a lower accuracy rate, which is not approaching 100%. 11 For the results of our study regarding STR CODIS loci alleles on paternity test in Human Genetic Study group ITD Universitas Airlangga, the highest percentage of locus alleles are as follows: TPOX allele 9 (34%), D3S1358 allele17 (35%), FGA allele 21 (36%), D5S818 allele 11 (33%), CSF1PO allele 9 (36%), DS820 allele 9 (38%), D8S1179 allele 12 (50%), THOI allele 9 (27%), vWA allele 18 (22%), D13S317 allele 8 (44%), D16S539 allele 10 (29%), D18S51 allele 16 (34%), and D21S11 allele 31(64%) (Table 2).…”

Section: Discussionmentioning

confidence: 99%

“…This comparison makes use of, among others, DNA from the siblings of the victims or the perpetrators when no comparison from the parents or children is possible. 1 A paternity test is a tool to determine whether a man is the biological father of someone and is a legal procedure for fatherhood. It is challenging to determine paternity because there are a lot of things that need to be confirmed.…”

Section: Introductionmentioning

confidence: 99%

Paternity Test Through Kinship Analysis as Forensic Identification Technique

Yudianto¹,

Setiawan

Sumino³

2021

mkb

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Paternity tests is often faced with the unavailability of information from the father, mother, or child as a comparison in forensic DNA examination process. Therefore, comparisons with information from close family lines are needed, for example from the victim's siblings or the perpetrator if there are no comparisons from parents or siblings. This study was conducted by the Human Genetic Study Group of Airlangga University in its campus from January to April 2020. The aim of the study was to analyze the use of kinship analysis in paternity test through STR CODIS examination on siblings. This was an observational laboratory study with a temporary design. DNA sample extraction level and purity results were measured with the mean DNA sample level of 675±5.35ng/µL, while the purity values ranged from 1.05 to 1.86. The paternity test principle is based on comparison process between the parents’ alleles with the child’s alleles. However, if the parents’ alleles are not available, the siblings’ alleles can be used as a comparison for identification purpose, which is known as kinship analysis. Statistically, full siblings have a 2 alleles accuracy probability of [0.25] 25%, which was the same as not having the same allele or 0 allele, while 1 allele accuracy reached 50%. All CODIS STR loci had the highest percentage of 2 allele sharing. Therefore, it is recommended to use sibling or kinship analysis if both parents are absent.

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Analysis of seven STR human loci for paternity testing by microchip electrophoresis

Abstract: ABSTRACT

Cited by 8 publications

References 8 publications

Rapid Stain Biomarker : A Novel Tool for the Forensic Detection of Saliva (Effect of Time and Surface on its Reliability )

Rapid Stain Biomarker : A Novel Tool for the Forensic Detection of Saliva (Effect of Time and Surface on its Reliability )

Understanding mosquito host-choice behaviour: a new and low-cost method of identifying the sex of human hosts from mosquito blood meals

Paternity Test Through Kinship Analysis as Forensic Identification Technique

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