Analysis of short-chain acids from bacteria by gas-liquid chromatography with a fused-silica capillary column

Abstract

The use of a flexible, fused-silica capillary column for gas-liquid chromatographic analysis of short-chain acids from bacteria is illustrated with a standard acid mixture and with a derivatized extract of culture medium from Clostridium difficile.

Bacterial composition of murine fecal microflora is indigenous and genetically guided

“…In earlier studies, the GLC of stool samples has proved suitable for reliable analysis of a large number of samples in a short time [15,16]. In this study, GLC was performed using an HP 6890 gas chromatograph with an Ultra 2 (Crosslinked 5% PH ME Siloxane) 25 mU0.2 mm column (HP 19091B) combined with HP ChemStation analysis software.…”

Characteristics of ‘atypical’, cytochrome oxidase‐negative Aeromonas salmonicida isolated from ulcerated flounders (Platichthys flesus (L.))

“…Experimental conditions. Gas liquid chromatography (GLC) of the bacterial cellular fatty acids was performed as described previously (Moss and Nunez-Montiel 1982 ;Eerola and Lehtonen 1988). The bacteria were cultured for GLC on fastidious anaerobic agar (BBL Microbiology Systems), scraped from the plates, saponified by heating for 30 min at 100°C in 15% (w/v) NaOH in 50% aqueous methanol, methylated with methanol/HCl and analysed as described earlier (Eerola and Lehtonen 1988).…”

“…All G L C profiles were compared as pairs to calculate similarity indices between individual sample pairs. These values were further analysed by cluster analysis and presented as dendrograms (Moss and Nunez-Montiel 1982) in order to assess the success of the GLC analysis in assigning strains belonging to the same species as the appropriate clusters. In addition, mean values and standard deviations were calculated for the similarity indices within and between each species group.…”

Gas chromatographic detection of sulphur compounds as methyl esters after growth of anaerobic bacteria in broth media

“…Thus, the data allowed us to identify these compounds as methyl esters of 3-(methy1thio)-propanoic acid for the U1 peak and 4-(methy1thio)-butanoic acid for the U2 peak. Unfortunately, no standard acids were available for comparison but, on the one hand, the mass spectrum of methyl 3-(methy1thio)-propanoate was analogous to the spectrum of authentic compound attainable in the computer library (EPA/NIH) and, on the other hand, these acids have already been described as trifluoroacetyl butyl ester derivatives from spent medium of a C. difjicile strain (Moss & Nunez-Montiel 1982). According to these authors, when this strain was grown in the medium supplemented with 0.4% (w/v) DL-methionine, the relative amounts of both acids increased significantly.…”