Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)

Ashkani, Sadegh; Rafii, Mohd Y.; Meon, Sariah; Akmar, A. Siti Nor; Rusli, Ibrahim; Harun, Abdul Rahim; Latif, Abdul

doi:10.4238/vol10-3gmr1331

Cited by 43 publications

(38 citation statements)

References 30 publications

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“…Plant material and crossing scheme BC 2 F 1 population derived from a cross between resistant rice variety, Pongsu Seribu 2, and the susceptible variety MR219 was genotyped with selected SSR markers tightly linked to blast resistance genes (Ashkani et al 2011). The general backcrossing steps were followed until BC 2 F 1 population.…”

Section: Methodsmentioning

confidence: 99%

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Tanweer

Rafii

Sijam

et al. 2015

Australasian Plant Pathol.

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The study was conducted to identify the suitable SSR markers in relation to blast disease of rice. Backcross breeding was applied and BC 2 F 1 generation was produced using rice variety Pongsu Seribu 2 as blast resistant parent and MR219 as susceptible parent. Eleven SSR markers related to blast resistance genes (Pi gene) were found highly polymorphic between the parental lines. According to single-gene model only two markers, RM208 and RM206, fitted to the expected test cross ratio (1:1) in BC 2 F 1 generation with the relation to resistant and susceptible plants. Further BC 2 F 1 population carrying 320 plants were inoculated with the highly virulent pathotype P7.2 of Magnaporthe oryze. Chi-square (χ2) test for single-gene model showed goodness of fit (p=0.45) to the expected segregation ratio (1:1) for phenotypic segregation in BC 2 F 1 population. In marker segregation analysis two markers RM208 (χ2=1.513, p=0.218) and RM206 (χ2=0.613, P=0.433) clearly produced goodness of fit to the expected test cross ratio (1:1) for the single-gene model. The present finding could help the rice breeders to monitor the blast resistance genes with appropriate and highly segregating SSR markers in rice varietal development program against blast disease.

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Section: Methodsmentioning

confidence: 99%

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Tanweer

Rafii

Sijam

et al. 2015

Australasian Plant Pathol.

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“…The gels were documented using Alpha Imager 1220 (Alpha Innotech, CA, USA). Microsatellite or Simple sequence repeat (SSR) markers were used for the rice lines selection (Temnykh et al, 2001;McCouch et al, 2002;Ashkani et al, 2011).…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

Performance of Yield and Yield Contributing Characteristics of Bc2f3 Population With Addition of Blast Resistant Gene

et al. 2015

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The study was carried out in the University Putra Malaysia (UPM) Rice Research Centre to evaluate the yield performance of newly developed selected blast resistant plants of BC 2 F 3 generations derived from a cross between MR263, a high yielding rice variety but blast susceptible and Pongsu Seribu 1, donor with blast resistant (Pi-7(t) and genes and qLN2 QTL), Malaysian local variety. On the basis of assessed traits, the plants 12, 6, 7, 5, 21, 22, 5, 26, 11, 8, 10, 13 and 15 had the higher yield, blast resistant and good morphological traits. More than 70% heritability was found in days to maturity, plant height, tiller numbers per hill, and panicle per hill, 80% heritability was found in filled grain and yield per hill and more than 90% heritability was found in grain length, grain width and seed weight. Cluster analysis based on the traits grouped 30 plants along with MR263 into seven clusters. According to PCA, the first four principal components account for about 69.3% total variation for all measured traits and exhibited high correlation among the characteristics analyzed.Index terms: Yield; rice; fungi resistance. RESUMOO estudo foi realizado no Centro de Arroz da Universidade Putra Malásia (UPM), para avaliar o desempenho produtivo de plantas resistentes a brunose do arroz recém desenvolvidas de BC 2 F 3 gerações derivadas do cruzamento entre MR263, uma variedade de arroz de alta produtividade, embora suscetível a brunose e Ponsu Seribu 1, doador com resistência a brunose e genes e qLN2 QTL], e também uma variedade local da Malásia. Com base nas características avaliadas, as plantas 12, 6, 7, 5, 21, 22, 5, 26, 11, 8, 10, 13 e 15 apresentaram o maior rendimento, resistência a brunose e boas características morfológicas. Mais de 70% de herdabilidade foram encontradas em dias para maturação, altura da planta, número de perfilhos por cova, e panículas por cova, 80% de herdabilidade foram encontradas em grãos e rendimento por colina e mais de 90% de herdabilidade foi observada em grãos, largura de grãos e peso das sementes. A análise de agrupamento com base nos traços agruparam 30 plantas, juntamente com MR263 em sete clusters. De acordo com o PCA, os quatro primeiros componentes principais responsáveis por cerca de 69,3% da variação total para todas as características medidas apresentaram alta correlação entre as características analisadas. Termos para indexação: Rendimento; arroz; resistência fúngica.

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“…The four-leaf stage seedlings were placed in inoculation chambers and inoculated with a conidial suspension (5 x 10 4 conidia/ mL). The inoculated plants were kept in the chambers at 26°C with 95% relative humidity and darkness for 24 h. They were then transferred to the greenhouse (28°-30°C/day and 20°-22°C/ night) for disease incubation, at 100% relative humidity, by intermittently spraying water for 1-2 min every 2 h (Ashkani et al, 2011). Each line was inoculated in two independent experiments, and three replications were conducted for each experiment.…”

Section: Pathogen Inoculation and Disease Evaluationmentioning

confidence: 99%

Population structure analysis and association mapping of blast resistance in indica rice (Oryza sativa L.) landraces

Ah³

et al. 2016

Genet. Mol. Res.

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ABSTRACT. Rice blast caused by Magnaporthe oryzae is one of the most devastating rice diseases worldwide. To understand the genetic diversity of indica landrace accessions and identify simple sequence repeat (SSR) markers that are associated with blast resistance, a population of 276 indica landraces from across the world was constructed. This population was then used to evaluate the blastresistance phenotype through artificial inoculation under controlled conditions in 2012 and 2013. The genetic diversity and association of the population with resistance were analyzed by examining the phenotype for 160 SSR markers distributed on 12 rice chromosomes. The 276 accessions were classified into seven groups using model-and distance-based cluster analyses. Associations between SSR markers and blast resistance showed that 26 SSR markers were significantly associated with blast resistance in 2012 and 2013 (P < 0.01) and that the phenotypic variation ranged from 2.68 to 13.11%. Nineteen of the markers associated with blast resistance were located in regions where genes or quantitative trait loci (QTLs) have been previously reported, and seven were newly identified in this study. These results indicate that marker-trait association has potential advantages over classical linkage analysis and QTL mapping, and that these markers could be used for marker-assisted selection in rice blast-resistance-breeding programs.

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Analysis of simple sequence repeat markers linked with blast disease resistance genes in a segregating population of rice (Oryza sativa)

Cited by 43 publications

References 30 publications

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Identification of suitable segregating SSR markers for blast resistance in rice using inheritance and disease reaction analysis in backcross families

Performance of Yield and Yield Contributing Characteristics of Bc2f3 Population With Addition of Blast Resistant Gene

Population structure analysis and association mapping of blast resistance in indica rice (Oryza sativa L.) landraces

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