Analysis of steroids by high-resolution gas—liquid chromatography

Spontaneous Hypercortisolism without Cushing's Syndrome

“…Insulin levels in plasma were determined by means of radio-immuno-assay (13). Gas chromatographic analysis of urinary steroids (as methoximtrimethylsilylderivatives) was performed using glass capillary columns (14). Identification of peaks was obtained by using the Kovats-indices (15).…”

Methodology of the thyroid gland disease decision-making using profiling in steroid hormone pathway

“…We measured the creatinine concentration in the urine using the Jaffé method. Metabolic profile is a quantitative data acquired in test for the materials utilizing in metabolite urine [3]. The measured metabolite profile is consist of well-known hormones such as androsterone, dehydroepiandrosterone, DHEA, 2-hydroxyestradiol, 2-hydroxyestrone, 2-methoxyestrone and other unknown hormones.…”

“…The first approach on profiling was conducted to steroids in urine, sugars, sugar alcohols and organic acid participated in Krebs cycle [1] and the study on the steroid profile was carried out live [2][3][4][5][6] after introduction of analysis method using glass capillary column and GC-MS.…”

Derivatization in gas chromatography

“…Methoxime trimethylsilyl derivatives were prepared from several steroids isolated from urine (227). The retention indexes were measured for the methoxime trimethylsilyl derivatives of some corticosteroid metabolites at 250" on an SE-30 column using c28-c30, C30-C32, and C28-C32 alkane mixtures as standards.…”