Analysis of steroids by off-line computerized gas chromatography-mass spectrometry

Reimendal, Robert.; Sjövall, Jan

doi:10.1021/ac60309a014

Cited by 121 publications

(26 citation statements)

References 19 publications

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“…Axelson and Sjö-vall developed a derivative purification method based on lipophilic Sephadex [11] and this was utilized for many years by steroid profilers but was finally replaced by a water extraction method devised by Dr Norman Taylor. A key development around 1970 was the computerization of GC/MS data recording and processing, and soon all instrument operations were managed by data systems [12].…”

Section: Gc/msmentioning

confidence: 99%

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Shackleton

2010

The Journal of Steroid Biochemistry and Molecular Biology

196

116

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Section: Gc/msmentioning

confidence: 99%

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Shackleton

2010

The Journal of Steroid Biochemistry and Molecular Biology

196

116

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“…Spectra were recorded on magnetic tape using the incremental technique [13]. Background subtraction, normalization and calculation of deuterium content were made on an IBM 1800 computer [14]. In the analyses of deuterium content, 5 -10 spectra were recorded during the elution of a compound.…”

Section: Gas-liquid Chromatographymentioning

confidence: 99%

Deuterium‐Labelling of sn‐Glycerol 3‐Phosphate during Metabolism of [1‐²H₂]Ethanol in Rats

Curstedt

1974

European Journal of Biochemistry

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The incorporation of deuterium into different positions of sn-glycerol 3-phosphate in liver and into glucose in plasma of rats given [1-'H,]ethanol has been determined. The labelling of sn-glycerol 3-phosphate reached a steady state after about 1 min. The deuterium excess was about 5 atoms % at C-1, 15 atoms% at C-2, and 5 atoms % at C-3. Plasma glucose contained less than 2 atoms % deuterium. Administration of glucose or fructose did not influence the labelling of sn-glycerol3-phosphate. The results are compared with those obtained for the glycerol moiety of phosphatidyl cholines. It is concluded that there is more than one pool of sn-glycerol3-phosphate in the liver, and that the major part has a glycolytic origin.Metabolism of ethanol leads to an increased concentration of NADH in the liver [l]. This results in increased concentrations of the reduced member of several redox couples. Thus, there is an increase of hepatic sn-glycerol 3-phosphate which might be the reason for the accumulation of lipids seen in the liver during ethanol metabolism [2,3]. To study this question, the transfer of deuterium from [ l-2H,]ethanol to the glycerol moiety of phosphatidyl cholines was measured and it was found that the deuterium excess at C-2 of the glycerol moiety became 2-3 times higher than that at C-2 of lactate and malate [4]. Since lactate, malate and sn-glycerol 3-phosphate are considered to be in equilibrium with a common'cytosolic NADH pool [5,6] this finding indicated a coupling between ethanol oxidation and formation of sn-glycerol 3-phosphate serving as precursor of the phosphatidyl cholines. It was therefore of importance to determine whether the deuterium excess at C-2 of hepatic sn-glycerol 3-phosphate resembled that of malate or that of the glycerol moiety of phosphatidyl cholines.In order to obtain further information about the origin of sn-glycerol 3-phosphate, the deuterium incorporation into a potential precursor, glucose, has also been determined. Furthermore, glucose and fruc-

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“…Further analysis was performed as reported previously (Shackleton & Gustafsson, 1971). Mass spectra were recorded on magnetic tape with the incremental mode of operation and were then processed in an IBM 1800 computer (Reimendal & Sjovall, 1972). A compound was considered identified onlyif it had the same mass spectrum and g.l.c.…”

Section: Methodsmentioning

confidence: 99%

The metabolism of steroids in the fatty liver induced by orotic acid feeding

Carrella

Björkhem

Gustafsson

et al. 1976

Biochemical Journal

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1. The metabolism of 4-[4-14C]androstene-3, 17-dione, 4-[4-14C]pregnene-3,20-dione, 5a-[4-14C]androstane-3a,17f8-diol, [4-4C]cholesterol, 7a-hydroxy4-6fi-3H]cholesten-3-one, 5fi-[7fi-3H]cholestane-3a,7U-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty livers and subnormal concentrations of cholesterol and triglycerides in serum. 3. The 61)-and 7a-hydroxylation of 4-androstene-3,17-dione, the 2a-, 21)-and 18-hydroxylation of 5a-androstane-3a,17fl-diol, and the 5a-reduction of 4-androstene-3,17-dione and 4-pregnene-3,20-dione were decreased by 40-50% in orotic acid-fed rats. Other oxidative and reductive reactions of the steroid hormones were not significantly affected. 4. The 12ax-hydroxylation of 7a-hydroxy4 cholesten-3-one was decreased by about 50 %, whereas the 7r-hydroxylation ofcholesterol and the 26-hydroxylation of 50i-cholestane-3a,7a-diol were not significantly decreased. The 6fi-hydroxylation of lithocholic acid was stimulated by 40%. 5. The r.esults are discussed in relation to present knowledge of the hepatic drug-metabolizing enzymes and to the recent findings of an abnormal bile acid metabolism in liver disease.

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Analysis of steroids by off-line computerized gas chromatography-mass spectrometry

Cited by 121 publications

References 19 publications

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Deuterium‐Labelling of sn‐Glycerol 3‐Phosphate during Metabolism of [1‐²H₂]Ethanol in Rats

The metabolism of steroids in the fatty liver induced by orotic acid feeding

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Analysis of steroids by off-line computerized gas chromatography-mass spectrometry

Cited by 121 publications

References 19 publications

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Clinical steroid mass spectrometry: A 45-year history culminating in HPLC–MS/MS becoming an essential tool for patient diagnosis

Deuterium‐Labelling of sn‐Glycerol 3‐Phosphate during Metabolism of [1‐2H2]Ethanol in Rats

The metabolism of steroids in the fatty liver induced by orotic acid feeding

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Deuterium‐Labelling of sn‐Glycerol 3‐Phosphate during Metabolism of [1‐²H₂]Ethanol in Rats