Analysis of structural changes in bleached keratin fibers (black and white human hair) using Raman spectroscopy

Kuzuhara, Akio

doi:10.1002/bip.20453

Cited by 74 publications

(88 citation statements)

References 23 publications

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“…Atomic interdiffusion quantum confinement stress effects enable Raman Spectroscopy. This is used to investigate the mechanism leading to the reduction of tensile strength of permanent waved human hair [36]; the internal structure changes in virgin human black hair keratin fibers that undergo aging [37]; the influence of chemical treatments (reduction, heating, and oxidation) on keratin fibers; and the structure of virgin white human hair resulting from a permanent hair straightening [10,38].…”

Section: Raman Spectroscopymentioning

confidence: 99%

“…Protein loss [11,12,[44][45][46][47][48]; Electrophoresis [13,25]; Scanning Electron Microscopy [5,[19][20][21][22]; Transmission Electron Microscopy [6,24,25]; Atomic Force Microscopy [7,26,[28][29][30][31]; Optical Coherence Tomography [8,33]; RAMAN spectroscopy [10,[32][33][34] Hair Shine-Gloss Diffuse reflectance spectrophotometry [14,33,43,46,50] Easy to comb Combing resistance [12,64,65] Strength properties Resistance to breakage [12,14,16,33,61,62] Photoprotection Photoluminescence spectroscopy [40][41][42][43] Long-lasting color Diffuse reflectance spectrophotometry [14,…”

Section: Damage Hair Reductionmentioning

confidence: 99%

“…Properties and effects of cosmetic products on hair fiber can be assessed through many methods, such as Scanning Electron Microscopy [5], Transmission Electron Microscopy [6], Atomic Force Microscopy [7], Optical Coherence Tomography [8], Infrared Spectroscopy [9], Raman Spectroscopy [10], Protein Loss [11,12], Electrophoresis [13], color and brightness [14], Thermal Analysis [15] and measuring mechanical resistance against combing and elasticity [12,16].…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Methodologies to Evaluate the Effects of Hair Care Products on Hair Fiber

2017

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Consumers use different hair care products to change the physical appearance of their hair, such as shampoos, conditioners, hair dye and hair straighteners. They expect cosmetics products to be available in the market to meet their needs in a broad and effective manner. Evaluating efficacy of hair care products in vitro involves the use of highly accurate equipment. This review aims to discuss in vitro methodologies used to evaluate the effects of hair care products on hair fiber, which can be assessed by various methods, such as Scanning Electron Microscopy, Transmission Electron Microscopy, Atomic Force Microscopy, Optical Coherence Tomography, Infrared Spectroscopy, Raman Spectroscopy, Protein Loss, Electrophoresis, color and brightness, thermal analysis and measuring mechanical resistance to combing and elasticity. The methodology used to test hair fibers must be selected according to the property being evaluated, such as sensory characteristics, determination of brightness, resistance to rupture, elasticity and integrity of hair strain and cortex, among others. If equipment is appropriate and accurate, reproducibility and ease of employment of the analytical methodology will be possible. Normally, the data set must be discussed in order to obtain conclusive answers to the test.

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Section: Raman Spectroscopymentioning

confidence: 99%

Section: Damage Hair Reductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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In Vitro Methodologies to Evaluate the Effects of Hair Care Products on Hair Fiber

2017

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“…The direct characterization of keratin fibers has been performed using X-ray diffraction, 1,[7][8][9][10][11][12] solid state NMR, 8,13 Raman, [14][15][16][17][18][19][20][21][22][23][24][25][26][27][28] and Infrared spectroscopy. 14,16,17 Information about the amorphous region (the cuticle and matrix) of…”

Section: Introductionmentioning

confidence: 99%

“…On the other hand, when directly characterizing the cuticle and cortex structure of a single keratin fiber, the analytical technique using a Raman microscope is effective as it can be measured at a spot diameter of 1 lm. [20][21][22][23][24][25][26] Most importantly, by using this method, information about the crystalline fibrous (microfibril) and amorphous (matrix) structures existing in the cortex region can be obtained. However, up to now, Raman spectroscopic analysis had been limited to white human hair, [20][21][22][23][24][25][26] which has no melanin granules, and blond human hair, 27,28 which has few melanin granules.…”

Section: Introductionmentioning

confidence: 99%

Analysis of internal structure changes in black human hair keratin fibers with aging using Raman spectroscopy

Kuzuhara¹,

Fujiwara²,

Hori³

2007

Biopolymers

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To investigate the internal structure changes in virgin black human hair keratin fibers due to aging, the structure of cross‐sections at various depths of virgin black human hair (sections of new growth hair: 2 mm from the scalp) from a group of eight Japanese females in their twenties and another group of eight Japanese females in their fifties were analyzed using Raman spectroscopy. For the first time, we have succeeded in recording the Raman spectra of virgin black human hair, which had been impossible due to high melanin granule content. The key points of this method are to cross‐section hair samples to a thickness of 1.50‐μm, to select points at various depths of the cortex with the fewest possible melanin granules, and to optimize laser power, cross slit width as well as total acquisition time. The reproducibility of the Raman bands, namely the α‐helix (α) content, the β‐sheet and/or random coil (β/R) content, the disulfide (SS) content, and random coil content of two adjoining cross‐sections of a single hair keratin fiber was clearly good. The SS content of virgin black human hair from the Japanese females in their fifties for the cortex region decreased compared with that of the Japanese females in their twenties. On the other hand, the β/R and α contents of the cortex region did not change. © 2007 Wiley Periodicals, Inc. Biopolymers 87: 134–140, 2007.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com

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Skin Pharmacology and Cosmetic Science Applications of IR Spectroscopy, Microscopy, and Imaging

Mendelsohn

Flach

Moore³

et al. 2010

Raman, Infrared, and Near‐Infrared Chemical Imaging

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The rapid growth in biomedical applications of vibrational microscopy and imaging is in large part due to the vast amount of information inherent in the complete IR or Raman spectrum acquired from each pixel during the measurement. For characterization of tissues and cells, this spectral information directly monitors elements of molecular and supramolecular structure in the sample constituents. This situation is in sharp contrast to imaging techniques based on electronic spectroscopy, that is, absorption or fluorescence, where the relationship between the spectral properties of the chromophore and the molecular structure of the tissue in which it is located is generally not available. As an example (described later in this chapter) of the relationship between the spectra and the molecular structure information, IR images acquired during the healing of wounded skin permit us to distinguish the spatial distribution of collagen from keratin, and even more important, permit us to distinguish between the various forms of keratin in cells that are activated during reepithelialization of the wounded area [1].For the past several years, our laboratories have utilized IR spectroscopy, microscopy, and imaging to monitor the biophysics and pharmacology of the skin barrier and the spatial distribution of the major constituents in hair [2][3][4][5][6][7]. This chapter presents an overview of how to effectively use these technologies to generate useful molecular and supramolecular structure information from tissues and cells. Our general approach is reductionist and predicated upon the reasonable assumption that molecular structure information extracted from IR spectra collected from purified tissue components provides an appropriate basis for interpretation of tissue spectra. To illustrate this approach for skin, we demonstrate the sensitivity of the IR spectra of purified ceramides (a major skin lipid class) to lipid chain conformation and packing. We next utilize this information to interpret a structural transition in intact full thickness stratum corneum (SC). This structural information is used to track the kinetics of the restoration of the skin barrier following its disruption by thermal perturbation. Finally, we move to the imaging mode and demonstrate the feasibility of imaging conformational order in exogenous lipid vesicles following their application to skin.Subsequent to studies of the permeability barrier in skin, we demonstrate the use of IR microscopy to track biochemical alterations in single cells through evaluation of natural moisturizing factor (NMF), an important hydration control mechanism in corneocyte biology. Finally, two applications of IR imaging are presented. The first is a cosmetic science experiment detailing the spatial distribution of the lipid and protein constituents in hair. The second is an application to the healing of skin wounds, in which we track temporal and spatial changes in the distribution of skin proteins that Raman, Infrared, and Near-Infrared Chemical Imaging Edited by Slobodan...

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Analysis of structural changes in bleached keratin fibers (black and white human hair) using Raman spectroscopy

Cited by 74 publications

References 23 publications

In Vitro Methodologies to Evaluate the Effects of Hair Care Products on Hair Fiber

In Vitro Methodologies to Evaluate the Effects of Hair Care Products on Hair Fiber

Analysis of internal structure changes in black human hair keratin fibers with aging using Raman spectroscopy

Skin Pharmacology and Cosmetic Science Applications of IR Spectroscopy, Microscopy, and Imaging

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