Analysis of T cell receptor V_β gene usage in primary mixed lymphocyte reactions : evidence for directive usage by different antigen‐presenting cells and Mls‐like determinants on T cell blasts

Abstract

The usage of four different T cell receptor (TcR) V beta gene families within normal, non-primed T cell populations in response to various types of antigen-presenting cells (APC) in primary mixed lymphocyte reaction has been studied. We demonstrate that distinct patterns of V beta gene usage are obtained within a given T cell population in response to different types of APC with the same allo-H-2. When responder T cells are stimulated with one type of allogeneic APC, from various H-2-disparate mice, the same V beta gene preference is observed. Furthermore, when H-2- and Mls-mismatched APC gene used as stimulators, the Mls-associated V beta 6 and V beta 8.1 gene families are highly elevated in response to both B and T cell blasts from certain Mls-positive strains. The results demonstrate that different types of allogeneic APC have the capacity to generate biases in TcR V beta gene usage and imply that functional Mls-like determinants are presented by T cell blasts. The findings are discussed with respect to TcR-major histocompatibility complex interactions in allostimulation.

CD4 expression is differentially required for deletion of MLS-1a-reactive T cells.

“…K. Kishihara and M. Schilham for critical reading of the manuscript. (18)(19)(20), and that the in vitro anti-Mls-1a response of CD8+ V06+ T cells is MHC class II dependent (21). Our results now demonstrate that CD4 expression is not absolutely required for Mls-1a-mediated clonal deletion of CD8+ V06+ T cells.…”

The mouse mammary tumour virus long terminal repeat encodes a type II transmembrane glycoprotein.

“…For example, CD8+ T cells have been shown to be potent inducers of tolerance to MlS-la (and Mls-3a) , while they are generally thought to be class II negative. Similarly, T cell blasts, presumably class II negative, can stimulate Mls responses (Larsson-Sciard et al, 1990). Finally, intercellular transfer of Mls antigens in vivo has been observed in irradiation bone-marrow chimeras (Pullen et al, 1988;Spieser et al, 1989), although contested by others (Webb et al, 1989).…”

Mls presentation by peritoneal cavity B cells

“…A variety of lymphoid and myeloid cells present endogenous SAgs and promote T cell activation or tolerance (Larsson-Sciard et al, 1990;Luther and AchaOrbea, 1997;Molina et al, 1989;Webb and Sprent, 1990;Webb et al, 1989). Although CD8 + and CD4 + T cells have been shown to present Mls (Larsson-Sciard et al, 1990;Webb and Sprent, 1990), most studies have focused upon the more traditional APC.…”