The temperature of bearings has a significant impact on their service life and flexibility, so it becomes more important to analyze the temperature field of bearings in high-speed electric spindle machining applications. Scholars at home and abroad have completed the thermal characteristics analysis of high-speed bearings from multiple perspectives such as frictional heat generation, heat transfer, and thermal analysis, but there is less consideration of the effects of spin, rotation speed, and external loads on the temperature field, and the complex motion between various components inside the bearing is also ignored. There are even fewer transient thermal analyses of high-speed bearings using the ANSYS finite element analysis method. Therefore, this paper carries out a temperature field analysis of high-speed bearings considering the interactive effects of frictional heat generation. Firstly, the motion laws of bearings and the mechanism of friction generation are analyzed, and a high-speed bearing frictional heat generation calculation analysis model that conforms to actual working conditions is established. Based on the ANSYS finite element analysis software, the temperature field of high-speed bearings is analyzed, and the internal temperature field distribution law is obtained by exploring the effects of spin, rotation speed, and external loads on their temperature field. Finally, the paper presents experimental analysis results, which verify the effectiveness of the proposed calculation method and the established model.