Analysis of the active components and metabolites of Taohong Siwu decoction by using ultra high performance liquid chromatography quadrupole time‐of‐flight mass spectrometry

Abstract

Taohong Siwu Decoction is a classic Chinese medicine prescription for treatment of cerebral ischemia and gynecological diseases. However, the active ingredients of Taohong Siwu Decoction have not been identified. In this study, a ultra performance liquid chromatography quadrupole time-of-flight mass system was used to analyze the active components and metabolites of Taohong Siwu Decoction absorbed into the blood and the brain. A total of 39 active compounds and 90 metabolites were identified in the blood and brain by comparing retention times, accurate masses, fragmentation patterns, and literature data. The results showed that flavonoids (Carthamus tinctorius L), aromatic organic acids, and benzoquinones (Angelica sinensis (Oliv.) Diels and Ligusticumchuanxiong hort) were prominent active ingredients in Taohong Siwu Decoction. Furthermore, hydrolysis, glucuronidation, and sulfation were identified as the main metabolic pathways of Taohong Siwu Decoction in vivo. This was the first study to characterize the active components and metabolites of Taohong Siwu Decoction in the blood and brain using ultra performance liquid chromatography quadrupole time-of-flight mass.

Development and evaluation of poly adenosine 5′‐diphosphate‐ribose polymerase 1 immobilization‐based receptor chromatography

“…Sinapine and tubeimoside‐1, the bioactive compounds in Sinapis semen and Bolbostemma paniculatum , exhibit reversed multidrug resistance and suppress metastasis in BC through NF‐κB activation [7,8]. The extract of Cinnamomum cassia demonstrates high anti‐proliferative activity against BC cells [9–11]. These cases support the view that YHD could be a promising TCM for the treatment of BC.…”

“…These cases support the view that YHD could be a promising TCM for the treatment of BC. However, further studies are needed to explore the active ingredients and elucidate the in‐depth molecular mechanisms [10].…”

Systematic characterization of chemical constituents in Mahuang decoction by UHPLC tandem linear ion trap‐Orbitrap mass spectrometry coupled with feature‐based molecular networking

“…Traditional Chinese medicine (TCM) and its prescriptions, especially the classical prescriptions, have long been used in clinics in China, and are revitalized recently due to people's increasing health needs [1, 2]. Multiple approaches have been attempted to dig into the chemical components of TCM prescriptions [3–7]. LC‐MS, especially UHPLC‐high‐resolution MS (UHPLC‐HRMS), stands out from the other techniques in the chemical analysis of the complex systems, due to the advantages of high sensitivity, specificity, and abundant information [8–12].…”

A strategy for practical authentication of medicinal plants in traditional Chinese medicine prescription, paeony root in ShaoYao‐GanCao decoction as a case study

“…UHPLC, the advanced technology of LC, allows more rapid analysis and less consumption of organic solvent with higher efficiency and higher throughput [10,11]. On the one hand, state-of-the-art tandem high-resolution MS provides more accurate molecular weights and compelling data for the characterization of chemical compounds, which is one of preferable approaches in the deep analysis of TCM [12,13]. On the other hand, to get more practicability and cut costs, miniature mass spectrometers have been fully developed and play an important role in screening of illegal adulterations and chemical markers [14][15][16].…”