Analysis of the Activity and Expression of Cyclooxygenases COX1 and COX2 in THP-1 Monocytes and Macrophages Cultured with BiodentineTM Silicate Cement

Barczak, Katarzyna; Palczewska-Komsa, Mirona; Nowicka, Alicja; Chlubek, Dariusz; Buczkowska-Radlińska, Jadwiga

doi:10.3390/ijms21062237

Cited by 7 publications

(7 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…With respect to BD, the present study showed that it led to significantly higher stimulation of the monocytes and subsequent release of cytokines in comparison with other groups. Such finding contrasts with previous results from in vitro study (Barczak, Palczewska‐Komsa, Lipski, et al, 2020; Barczak, Palczewska‐Komsa, Nowicka, et al, 2020). In vivo studies showed that BD resulted in adequate biological response, that is as similar as to MTA.…”

Section: Discussioncontrasting

confidence: 99%

“…In respect to MTA, the present study showed that it was associated with significantly lower levels of inflammatory cytokines released by monocytes compared to Biodentine. Comparable findings were previously reported in an in vitro study where MTA product did not trigger THP‐1 Macrophages to release inflammatory mediators (Barczak, Palczewska‐Komsa, Lipski, et al, 2020; Barczak, Palczewska‐Komsa, Nowicka, et al, 2020). Contrasting results were found by another in vitro study where they found that MTA can trigger the release of pro‐inflammatory molecules namely IL‐1β, TNF‐α and IL‐6 by RAW 264.7 macrophages (Yuan et al, 2018).…”

Section: Discussionsupporting

confidence: 86%

See 1 more Smart Citation

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

et al. 2023

View full text Add to dashboard Cite

Aim Inflammatory‐regenerative cell interaction is believed to mediate hard tissue formation. This study aimed to investigate the interaction between human inflammatory monocytes with human regenerative fibroblasts after exposure to different calcium silicate materials. Methodology Human monocytes were cultured on three materials, polystyrene (PS), mineral trioxide aggregates (MTA) and biodentine (BD), in the presence or absence of lipopolysaccharide (LPS). Half of the monocyte‐conditioned media (MoCM) of each group was used to analyse inflammatory cytokine secretion, namely TNF‐α, IL‐1β, IL‐1RA and IL‐6. The remaining MoCM was used to culture recipient fibroblasts, measuring the cell number (proliferation) and levels of alkaline phosphatase (differentiation) and lactic acid dehydrogenase (cytotoxicity). Results In absence of LPS, MTA was associated with higher secretion of TNF‐α and lower secretion of IL‐1β, while BD triggered higher secretions of both cytokines when both materials were compared to control (PS). When LPS was added, higher levels of all analysed cytokines were observed in the PS and BD groups, whereas for the MTA group, only TNF‐α and IL‐6 were increased. Fibroblasts responded differently to the MoCM from the different groups, revealing significant increases in proliferation and differentiation capacities, particularly when cultured in CM from monocytes exposed to MTA. The morphological evaluation revealed different patterns of fibroblast shape and spread in the different MoCM groups. Conclusion Calcium silicate materials modulate the monocyte inflammatory response, which subsequently induce differential effects on the recipient fibroblasts. MTA appears to promote the secretion of prodifferentiation signals from the monocytes, which are received by fibroblasts, promoting their proliferation and differentiation. The model represents a promising tool to evaluate the interaction of different cells in response to different materials.

show abstract

Section: Discussioncontrasting

confidence: 99%

Section: Discussionsupporting

confidence: 86%

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

et al. 2023

View full text Add to dashboard Cite

show abstract

“…For THP-1 cells, our data showed the successful differentiation of these monocytes into macrophages based on the cell surface markers' expression as analyzed by flow cytometry, and these results are in alignment with a previous study [34]. Macrophages are important innate immune cells that are associated with two distinct types: a proinflammatory subset M1with prototypic macrophage functions such as inflammatory cytokine production and bactericidal activity and an anti-inflammatory subset M2 linked with wound healing and tissue repair processes [35].…”

Section: Biomed Research Internationalsupporting

confidence: 89%

The Immunomodulatory and Regenerative Effect of Biodentine™ on Human THP-1 Cells and Dental Pulp Stem Cells: In Vitro Study

Abuarqoub

Aslam

Zaza

et al. 2022

BioMed Research International

View full text Add to dashboard Cite

Background. Pulp tissue affected by deep caries and trauma can be protected by vital pulp therapies in which pulp regeneration success depends on the degree of pulp inflammation and the presence of regenerative signals. Reparative dentinogenesis requires dental pulp stem cell (DPSC) activity which can be stimulated by many bioactive molecules to repair the dentine, mediating a balance between the inflammatory response and the reparative events. Therefore, this study was performed in order to investigate the immune-inflammatory effect of Biodentine capping material on DPSCs and macrophages. Method. THP-1, a human monocytic cell line, was differentiated to macrophages, and flow cytometry was used to analyze the expressions of specific macrophage markers. LPS-mediated infection was created for macrophages and DPSCs followed by treatment with Biodentine. CBA array was used to investigate the cytokine secretion followed by qPCR. Migration potential of treated DPSCs was also determined. Results. Our results showed that THP-1 cell line was successfully differentiated into macrophages as shown by surface marker expression. CBA array and qPCR results showed that Biodentine-treated DPSCs and macrophages upregulated anti-inflammatory cytokines and downregulated proinflammatory cytokines. Also, Biodentine enhances the migration potential of treated DPSCs. Conclusion. Biodentine capping material mediated the polarization of M1 to M2 macrophages suggestive of tissue repair properties of macrophages and enhanced the anti-inflammatory cytokines of DPSCs responsible for dentine-pulp regeneration.

show abstract

“…Shi et al, 2014 compared cytotoxic activity of iRoot BP Plus and MTA ProRooton human gingival fibroblasts in medium in vitro and evaluated the cells vitality. The results obtained in the aforementioned study demonstrate that cell vitality at different concentrations of iRoot BP Plus and MTA amounted to from 77.3% to 113.8% with no cytotoxic effects recorded in either of the materials [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

Barczak

Palczewska-Komsa

Lipski

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

The aim of the present study was to investigate the new silicate cement mineral trioxide aggregate (MTA Repair HP) with respect to its effect on the inflammation process involving the tooth and periodontal tissues. The composition of MTA Repair HP was supplemented with plasticizer agents which can have a negative effect on the modulation of tooth inflammation. The silicate-based material in question is widely used in regeneration of the pulp-dentin complex, treatment of perforations of various locations in the tooth, as well as in surgical treatment of the complications of periapical tissue. The improved bioceramic restorative cement can affect the expression of metalloproteinases MMP-2 and MMP-9 in monocytes/macrophages involved in modulation of inflammation and regenerative processes of the tooth and periodontal tissues. The novel aspect of the present study lies in the application of the model of THP-1 monocyte/macrophage and applying the biomaterial in direct contact with the cells. Hence, it provides a representation of clinical conditions with respect to regenerative pulp and periodontal treatment with the use of MTA Repair HP. A lack of macrophage activation (as measured with flow cytometry) was found. Moreover, the study identified a lack of expression stimulation of the studied metalloproteinases (with the use of Western blotting and fluorescent microscopy). Similarly, no increase in MMP-2 and MMP-9 concentration was found (measured by ELISA method) in vitro when incubated with MTA Repair HP. Based on the results it can be concluded that new MTA Repair HP does not increase the inflammatory response in monocytes/macrophages associated with the activity of the described enzymes. It can also be speculated that they do not affect the process of dentin regeneration in which MMP-2 and MMP-9 play significant roles.

show abstract

Analysis of the Activity and Expression of Cyclooxygenases COX1 and COX2 in THP-1 Monocytes and Macrophages Cultured with BiodentineTM Silicate Cement

Cited by 7 publications

References 49 publications

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

Inflammatory and differentiation cellular response to calcium silicate cements: An in vitro study

The Immunomodulatory and Regenerative Effect of Biodentine™ on Human THP-1 Cells and Dental Pulp Stem Cells: In Vitro Study

The Influence of New Silicate Cement Mineral Trioxide Aggregate (MTA Repair HP) on Metalloproteinase MMP-2 and MMP-9 Expression in Cultured THP-1 Macrophages

Contact Info

Product

Resources

About