Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS

Dai, Lulu; Guo, Nian; Liu, Yaqin; Shen, Shanshan; Ge, Qiu-Fu; Pan, Yuanjiang

doi:10.1016/j.cclet.2017.12.023

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…Use of a high‐resolution liquid‐phase mass spectrometer can effectively reduce errors and is thus one of the most effective methods to verify peptide sequences (Dai et al , ). In this study, HPLC‐MS/MS analysis was used to identify the peptide sequences of the peptide fractions before and after fermentation, and the results are summarised in Table S1 and Table , respectively.…”

Section: Resultsmentioning

confidence: 99%

Characterisation of antibacterial peptide fractions extracted from pomelo nucleus co‐incubated with Lactobacillus

Liu

Song

et al. 2020

Int J of Food Sci Tech

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Pomelo have been widely reported for its unique flavour and high nutritive value, whereas the antibacterial activity of pomelo nucleus peptides was still poorly understood. We characterised a co-incubation system of pomelo nucleus and Lactobacillus amylolyticus L6 to identify peptides of high application value. We first analysed the structure of pomelo nucleus peptides (GP) and co-incubated peptides (C-GP) by scanning electron microscopy, high-performance gel permeation chromatography, liquid chromatographytandem mass spectrometry and amino acid analysis. The results showed that the molecular weights, 89% of peptide sequences, and amino acid composition were different in the C-GP compared with the GP fraction, and the spatial structures were quite diverse; the C-GP peptide fraction presented irregular, amorphous and interwoven flocs. Notably, only C-GP had antimicrobial activity against Escherichia coli (minimum inhibitory concentration of 12.50 lg/mL). Further assessment of the mechanism suggested that the hydrophobic groups in the C-GP peptide fraction were inserted into the hydrophilic sites on the surface of the E. coli cell membrane, leading to the formation of holes and bending. These findings suggest the potential value of pomelo nucleus as a nutrient source.

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Section: Resultsmentioning

confidence: 99%

Characterisation of antibacterial peptide fractions extracted from pomelo nucleus co‐incubated with Lactobacillus

Liu

Song

et al. 2020

Int J of Food Sci Tech

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“…LC/MS/MS 30–35 is currently the most sensitive and selective method for detecting organic acids 36–40 . However, CCAs present a class of strongly polar compounds with no UV‐sensitive group in their structure, which is a challenge to analysts 41 .…”

Section: Introductionmentioning

confidence: 99%

“…However, no relevant investigation on the analysis of CCAs in rice has been reported so far. LC/MS/MS [30][31][32][33][34][35] is currently the most sensitive and selective method for detecting organic acids. [36][37][38][39][40] However, CCAs present a class of strongly polar compounds with no UV-sensitive group in their structure, which is a challenge to analysts.…”

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confidence: 99%

Determination of trace chelating carboxylic acids in rice by green extraction combined with liquid chromatography–mass spectrometry analysis and its application in the evaluation of old and new rice

Kang,

Wang,

Feng

et al. 2024

Rapid Comm Mass Spectrometry

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RationaleAccurate identification of old rice samples from new ones benefits their market circulation and consumers. However, the current detection methods are still not satisfactory because of their insufficient accuracy or (and) time‐consuming process.MethodsChelating carboxylic acids (CCAs) were selectively extracted from rice, by stirring with chelating resin and a dilute Na2CO3 solution. The green analytical chemistry guidelines for sample preparation were investigated by using the green chemistry calculator AGREE prep. The extractant was determined by liquid chromatography–mass spectrometry (LC/MS), and statistical analysis of the analytical data was carried out to evaluate the significance of the difference by ChiPlot.ResultsThe limit of quantitation for the CCAs is in the range of 1 to 50 ng/mL, with a reasonable reproducibility. The CCAs in 23 rice samples were determined within a wide concentration range from 0.03 to 1174 μg/g. Intriguingly, the content of citric acid, malonic acid, α‐ketoglutaric acid and cis‐aconite acid in new rice was each found to be distinctively higher than that in old rice by several times. Even mixtures of old and new rice were found to show much difference in the concentration of citric acid and malic acid.ConclusionA green analytical method has been developed for the simultaneous determination of CCAs by LC/MS analysis, and the identification of old rice samples from new ones was easily carried out according to their CCA content for the first time. The results indicated that the described method has powerful potential for the accurate identification of old rice samples from new ones.

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“…So far, the techniques used to detect 5ʹ-GMP are mainly ion chromatography, capillary electrophoresis and high-performance liquid chromatography [4][5][6]. Compared with traditional instrumental analytical techniques, electrochemical techniques offer attractive advantages such as higher sensitivity, faster response time, simpler instrumentation, and easier on-line detection without complicated pretreatment, expensive equipment, skilled personnel, and long analysis times.…”

Section: Introductionmentioning

confidence: 99%

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ-GMP

Huan

Wang

et al. 2022

Appl Biol Chem

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A sensitive electrochemical sensor for the selective detection of 5ʹ-guanylic acid (5ʹ-GMP) was prepared by combining sulfonated-multiwalled carbon nanotubes (SMWCNTs) and [Ru(bpy)2dpp]Cl2, which were dripped on the surface of a glass carbon electrode (GCE) immobilized with gold nanoparticles. The 5ʹ-GMP electrochemical biosensor was fabricated using [Ru(bpy)2dpp]2+/SMWCNTs/Au/GCE as working, Ag/AgCl as reference and Pt as auxiliary electrode connected by an electrochemical workstation. The modified electrode was characterized by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The results showed the sensor’s response current had the best peak shape and maximum peak when the pH of electrolyte was 3, scan speed of CV was in the range of 100 to 180 mV/s, and the enrichment time was in the range of 200 to 300 s. Under the optimum conditions, a linear analytical curve was obtained for 5ʹ-GMP concentrations in the range of 0.01 to 0.5 mmol L−1, with a detection limit of 0.0014 mmol L−1. The analytical results of the 5ʹ-GMP sensor were exhibited good consistent with the data from liquid chromatography. The sensor has good reproducibility, long-term stability and strong immunity to interference, and may be a powerful device for 5ʹ-GMP detection, with great advantages such as simple preparation and operation, low equipment cost.

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Analysis of the binding sites with NL-101 to amino acids and peptides by HPLC/MS/MS

Cited by 9 publications

References 18 publications

Characterisation of antibacterial peptide fractions extracted from pomelo nucleus co‐incubated with Lactobacillus

Characterisation of antibacterial peptide fractions extracted from pomelo nucleus co‐incubated with Lactobacillus

Determination of trace chelating carboxylic acids in rice by green extraction combined with liquid chromatography–mass spectrometry analysis and its application in the evaluation of old and new rice

An electrochemical sensor based on [Ru(bpy)2dpp]2+/SMWCNTs/Au modified glassy carbon electrode for the detection of 5ʹ-GMP

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