Analysis of the flagellin (hag) gene of alkalophilic Bacillus sp. C-125

Sakamoto, Yoh-Ichiro; Sutherland, Katharine J.; Támaoka, Jin; Kobayashi, Tetsuo; Kudo, Toshiaki; Horikoshi, Koki

doi:10.1099/00221287-138-10-2159

Cited by 13 publications

(4 citation statements)

References 29 publications

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“…This indicated that the central region of the leptospiral FlaB protein is highly variable in amino acid sequence and could be species specific. A similar sequence pattern was also found with other flagellins (25,29). The information contained in the central region may be useful for the design of a specific peptide or DNA probe to detect infection with leptospires.…”

supporting

confidence: 67%

Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis

Lin¹,

Surujballi²,

Nielsen³

et al. 1997

Infect Immun

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During the screening of antibodies to pathogenic leptospires, a murine monoclonal antibody (designated M138) was found to react with various serovars. An antigen of approximately 35 kDa from Leptospira interrogans serovar pomona, which reacted strongly with M138, was characterized by N-terminal amino acid sequencing and identified as a flagellin, a class B polypeptide subunit (FlaB) of the periplasmic flagella. The gene encoding the FlaB protein, flaB, was amplified from the genomic DNA of several pathogenic serovars by PCR with a single pair of oligonucleotide primers, suggesting that FlaB is highly conserved among these serovars. Cloning and sequence analysis of flaB from serovar pomona revealed that it contains an 849-bp open reading frame with a G ؉ C content of 46.88% which encodes a 283-amino-acid protein with a calculated molecular mass of 31.297 kDa and a predicted pI of 9.065. A sequence comparison of flagellin proteins revealed that the amino acid sequence is most variable in the central portion of the serovar pomona FlaB, which is believed to contain specific sequence information and which may thus be useful in the design of DNA or synthetic peptide probes suitable for the detection of infection with pathogenic leptospires.

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supporting

confidence: 67%

Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis

Lin¹,

Surujballi²,

Nielsen³

et al. 1997

Infect Immun

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“…The molecular mass of C. difficile flagellin, 39 kDa, is in the middle of the range of other characterized flagellin molecules, which have been reported to have molecular masses ranging from 15 to 62 kDa (Arnold et al, 1998 ;Joys, 1988 ;Sakamoto et al, 1992 ;Wilson & Beveridge, 1993). C. difficile flagellins did not display heterogeneity between the different strains studied nor did they have multiple molecular masses.…”

Section: Discussionmentioning

confidence: 88%

“…Prediction of antigenic determinants in FliC of C. difficile using the Hopp and Woods algorithm (Hopp & Woods, 1981) revealed the highest probability for the presence of such motifs in the central, variable region between aa 100 and 130. The central region has been proposed to be the region that is exposed to the outer environment (Sakamoto et al, 1992). Like other flagellins, the central portion has a surplus of acidic residues over basic residues and, by implication, a net negative charge.…”

Section: Discussionmentioning

confidence: 99%

A Clostridium difficile gene encoding flagellin The GenBank accession numbers for the sequences reported in this paper are AF065259 (strain 79-685) and AF077341 (strain VPI 10463).

et al. 2000

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“…DNA probes for sec Y (Kang et al 1992) and rpoA from strain C-125 hybridized to the same fragments 4A and 3S' ( Table 4). It was also found that probes for groEL (Xu et al 1996) and hag (Sakamoto et al 1992) hybridized to 3A and 4S, and llA and IS, respectively. 7).…”

Section: Mapping Of Genetic Locimentioning

confidence: 94%