Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis

Lin, Min; Surujballi, Om; Nielsen, Klaus; Nadin-Davis, Susan A.; Randall, Geoffrey

doi:10.1128/iai.65.10.4355-4359.1997

Cited by 13 publications

(6 citation statements)

References 31 publications

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“…It is a serogroup‐specific assay, but interpretation of the MAT is complicated by the high degree of cross‐reaction that occurs between different serogroups, especially in acute‐phase samples. This “paradoxical” reaction, in which the highest titres are detected to a serogroup unrelated to the infecting one, are also common and studied (Blanco, dos Santos, Galloway, & Romero, ; Lin, Surujballi, Nielsen, Nadin‐Davis, & Randall, ). The broad cross‐reactivity in the acute phase is followed by relative serogroup specificity in convalescent‐phase samples (Levett, ).…”

Section: Discussionmentioning

confidence: 93%

Serogroups and genotypes ofLeptospiraspp. strains from bovine aborted foetuses

Delooz

Czaplicki²,

Grégoire³

et al. 2017

Transbound Emerg Dis

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Leptospirosis is a global disease of animals, with potential major economic impact on livestock industry and important zoonotic capacities. The disease represents a major challenge in the developing countries as humans and animals frequently live in close association. The serovar Hardjo of Leptospira whose primary host is cattle has been studied extensively, but few data exist on other current circulating or emerging serotypes. To better understand the disease in cattle and how to prevent and/or control it, it is necessary to identify the genotype and the serotype of circulating Leptospira. This study presents results of several investigations performed on a historical Belgian collection of congenital jaundice in bovine aborted foetuses coming from the leptospirosis emerging episode of 2014 (Delooz et al., Transboundary and Emerging Diseases, 62, 2015, 124). The results revealed that L. Grippotyphosa and L. Australis were the most prevalent serogroups with, respectively, 17/42 and 13/42 positive microscopic agglutination test (MAT) during this emerging event associated with the same clinical pattern. The study also confirms that congenital jaundice is associated with L. kirscheneri and L. interrogans and provides the genotyping of DNA obtained from these two species.

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Section: Discussionmentioning

confidence: 93%

Serogroups and genotypes ofLeptospiraspp. strains from bovine aborted foetuses

Delooz

Czaplicki²,

Grégoire³

et al. 2017

Transbound Emerg Dis

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“…This gene encodes for flagellin, a class B polypeptide subunit of the periplasmic flagella. The sequence of flaB is also highly conserved among pathogenic serovars of Leptospira [68]. https://doi.org/10.1371/journal.pntd.0008074.g008 Similar to the lipL32 gene, the absence of flaB in the saprophytic strains allows this gene to be a good target for detecting pathogenic leptospires [50,69].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis

Lam

Low²,

Chee

2020

PLoS Negl Trop Dis

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BackgroundLeptospirosis is often difficult to diagnose because of its nonspecific symptoms. The drawbacks of direct isolation and serological tests have led to the increased development of nucleic acid-based assays, which are more rapid and accurate. A meta-analysis was performed to evaluate the diagnostic accuracy of genetic markers for the detection of Leptospira in clinical samples. Methodology and principle findingsA literature search was performed in Scopus, PubMed, MEDLINE and non-indexed citations (via Ovid) by using suitable keyword combinations. Studies evaluating the performance of nucleic acid assays targeting leptospire genes in human or animal clinical samples against a reference test were included. Of the 1645 articles identified, 42 eligible studies involving 7414 samples were included in the analysis. The diagnostic performance of nucleic acid assays targeting the rrs, lipL32, secY and flaB genes was pooled and analyzed. Among the genetic markers analyzed, the secY gene showed the highest diagnostic accuracy measures, with a pooled sensitivity of 0.56 (95% CI: 0.50-0.63), a specificity of 0.98 (95% CI: 0.97-0.98), a diagnostic odds ratio of , and an area under the curve of summary receiver operating characteristics curves of 0.94. Nevertheless, a high degree of heterogeneity was observed in this meta-analysis. Therefore, the present findings here should be interpreted with caution.

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“…The gene encoding the FlaB virulence factor, flaB , can be amplified from the genomic DNA of several pathogenic serovars. Cloning and sequence analysis indicated that flaB is suitable in the detection of infection by pathogenic leptospires [ 93 ].…”

Section: Recombinant Protein Vaccinesmentioning

confidence: 99%

Leptospirosis vaccines

2007

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Leptospirosis is a serious infection disease caused by pathogenic strains of the Leptospira spirochetes, which affects not only humans but also animals. It has long been expected to find an effective vaccine to prevent leptospirosis through immunization of high risk humans or animals. Although some leptospirosis vaccines have been obtained, the vaccination is relatively unsuccessful in clinical application despite decades of research and millions of dollars spent. In this review, the recent advancements of recombinant outer membrane protein (OMP) vaccines, lipopolysaccharide (LPS) vaccines, inactivated vaccines, attenuated vaccines and DNA vaccines against leptospirosis are reviewed. A comparison of these vaccines may lead to development of new potential methods to combat leptospirosis and facilitate the leptospirosis vaccine research. Moreover, a vaccine ontology database was built for the scientists working on the leptospirosis vaccines as a starting tool.

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Identification of a 35-kilodalton serovar-cross-reactive flagellar protein, FlaB, from Leptospira interrogans by N-terminal sequencing, gene cloning, and sequence analysis

Cited by 13 publications

References 31 publications

Serogroups and genotypes ofLeptospiraspp. strains from bovine aborted foetuses

Serogroups and genotypes ofLeptospiraspp. strains from bovine aborted foetuses

Diagnostic accuracy of genetic markers and nucleic acid techniques for the detection of Leptospira in clinical samples: A meta-analysis

Leptospirosis vaccines

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