Analysis of the Genetic Distance of Several Generations of Barley (Hordeum valulgare L) by RAPD-PCR Technique

Abstract

Random-amplified-polymorphic-DNA(RAPD) was assayed to detect the genetic variation of 6 barley generations from Iraq. Four primers generated a total of 17 scoreable bands in RAPD analysis) and resolving power, the three polymorphic primers differed (Rp). The use of RAPD marker systems to detect the genetic distance among barley generation was discovered to be beneficial. The RAPD dendrograms indicate a diverse grouping of 6 barely specimens, although we did see that certain groups were identical in several cases. As a result, the RAPD molecular markers reveal two genetic groups in the few specimens examined. Keywords. Barley, Genetic variation, RAPD-PCR.

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“…Therefore, species that are genetically close to each other show a similar distribution of bands, and species that are genetically distant from each other show a different distribution of bands (12). Alsaffar (13) showed, in a study of the genetic distance between 6 generations of barley, that he used the RAPD test using 17 random primers, and between the tests, 4 primers were able to combine and reveal the presence of genetic variation between the varieties. When Fadel (14) studying genetic variation in five barley varieties (Samir, Aksad, Amal, Buraq, and IPA265), used a PCR-RAPD assay with 12 random primers.…”