Analysis of the healing process in delayed tooth replantation after root canal filling with calcium hydroxide, Sealapex and Endofill: a microscopic study in rats

Abstract

The major concern in the therapeutics of tooth replantation refers to the occurrence of root resorption and different approaches have been proposed to prevent or treat these complications. The purpose of this study was to evaluate tissue response to delayed replantation of anterior rat teeth treated endodontically using calcium hydroxide, Sealapex, and Endofill without the placement of gutta-percha cones. Thirty rats had their right upper incisor extracted and maintained in dry storage for 60 min. After removal of the dental papilla, enamel organ, pulp tissue, and periodontal ligament remnants, the teeth were immersed in 2% sodium fluoride phosphate acidulated, pH 5.5, for 10 min. The root canals were dried with absorbent paper points and the teeth were assigned to three groups (n = 10) according to the filling material. Group I - calcium hydroxide and propyleneglycol paste, Group II - Sealapex, and Group III - Endofill. The sockets were irrigated with saline and the teeth were replanted. Replacement resorption, inflammatory resorption and ankylosis were observed in all groups. Although the occurrence of inflammatory resorption was less frequent in Group I, there were no statistically significant differences among the groups. It may be concluded that compared to the paste, filling the root canals with Sealapex and Endofill sealers without the placement of gutta-percha cones did not provide better results.

Efficacy of NiTi rotary instruments in removing calcium hydroxide dressing residues from root canal walls

“…Various biological properties have been attributed to this substance, such as antimicrobial activity, 1 high alkalinity, 2 inhibition of tooth resorption, 3 and tissue-dissolving ability. 4 To be effective, it has to be adequately placed and condensed in the root canal space.…”

Intracanal dressing and root canal filling materials in tooth replantation: a literature review

“…Gutta-percha is the most frequently used root canal filling material (9,28,36,(39)(40)(41)(42)(43) in combination with zinc oxide and eugenol-based sealers (21,22,34,44,45) and CH-based sealers, such as Sealapex (37,38,46). The use of mineral trioxide aggregate (MTA) has also been investigated in immediate tooth replantation (47).…”

“…Zinc oxide and eugenol-based sealers are the most widely used, despite the reports of intense/severe periapical inflammatory response to their use. In addition, these sealers have biocompatibility (52), which is an important propriety in cases of delayed replantation in which replacement resorption is expected and exposure of material is unavoidable (46). On the other hand, CH-based sealers may be a good option for replanted teeth because the gradual and slow release of hydroxyl and calcium ions from these materials maintains the high alkaline environment achieved with the placement of the intracanal dressing (71,72).…”

Evaluation of two formulations containing mineral trioxide aggregate on delayed tooth replantation: relevance of RANKL/RANK/OPG system

“…Various studies have also evaluated a series of substances as intracanal medications, including calcium hydroxide, Endofill and Sealapex [4], corticosteroids [5][6][7], bisphosphonates [8,9], and Ledermix paste Ò [10,11], although none of them was able to prevent root resorption.…”