Analysis of the immunomodulatory activity of excreted and secreted products from Haemonchus placei transition infective larvae (xL3)

Maza-Lopez, Jocelyn; Contreras-Ochoa, Carla; Reyes-Guerrero, David Emanuel; Encarnación‐Guevara, Sergio; Hernández‐Ortíz, Magdalena; Olmedo-Juárez, Agustín; Arellano, María Eugenia López

doi:10.1016/j.vetpar.2021.109512

Cited by 4 publications

(1 citation statement)

References 39 publications

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“…Teladorsagia circumcincta , Haemonchus placei and H. contortus are all parasitic on the fourth gastric mucosa of ruminants, and all are acid tolerant and resistant to gastrin digestion [ 49 , 50 ]. Multiple sequence alignment and phylogenetic analysis showed that the HcGOB gene is highly related to that of T. circumcincta and H. placei , respectively.…”

Section: Discussionmentioning

confidence: 99%

In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs)

et al. 2021

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Background Trehalose-6-phosphate phosphatase (TPP6) is a key enzyme in the trehalose biosynthesis pathway. The accumulation of TPP6 inside the body is harmful to the pathogen, but almost nothing is currently known about the function of TPP6 from Haemonchus contortus (CRE-GOB-1). Methods The H. contortus CRE-GOB-1 (HcGOB) gene was cloned and recombinant protein of GOB (rHcGOB) was expressed; transcription of the HcGOB gene at different developmental stages of H. contortus was then studied. The spatial expression pattern of the HcGOB gene in adult female and male worms was determined by both quantitative real-time PCR (qPCR) and immunofluorescence. The binding of the rHcGOB protein to goat PBMCs was assessed by immunofluorescence assay. The immunomodulatory impacts of rHcGOB on cell proliferation, nitric oxide generation and cytokine secretion were assessed by co-culture of rHcGOB protein with goat PBMCs. Results The HcGOB protein was transcribed in eggs, infective third-stage larvae (iL3s) and adults of H. contortus, with the highest transcript levels found in the egg stage. The transcript levels were significantly elevated in iL3s after manual desheathing. HcGOB was widely distributed in adult worms where it was mainly localized in the gut and gonads. rHcGOB was observed to bind to PBMCs and also to be recognized by sera collected from a goat infected with H. contortus. rHcGOB significantly activated the interleukin-10/transforming growth factor β/signal transducer and activator of transcription 3 (IL-10/TGF-β/STAT3) pathway in PBMCs while suppressing the transcription and expression of IL-4 and IL-17. Conclusions These results suggest that the HcGOB gene plays an important role in the development, parasitism and reproduction of H. contortus. The rHcGOB protein affected the immunomodulatory function of PBMCs in the in vitro study, suggesting that this protein would be a promising vaccine target. Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs)

et al. 2021

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In silico analysis of two Haemonchus spp. serine protease peptides (S28) and their immunomodulatory activity in vitro

Camas-Pereyra

Bautista-García

Ávila

et al. 2023

Molecular and Biochemical Parasitology

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Selection of Molecules with Immunological Potential from Excretory and Secretory Products from the Nematode Haemonchus placei by Cell Proliferation and Gene Expression Assays

Maza-Lopez¹,

Camas-Pereyra²,

López-Arellano³

et al. 2023

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The nematode Haemonchus placei is a pathogenic parasite, the most seriously affecting ruminant’s health and being responsible for enormous economic losses all over the world. The present protocol describes different in vitro techniques to select potential candidate antigens with immune-protective activity from excretory and secretory products (ESP) from H. placei transitory infective larvae (xL 3 ). ESP from xL 3 were obtained from the in vitro infective larvae (L 3 ) maintained in Hank’s medium at 37 °C with 5% CO 2 for 48 h. Then, the presence of ESP proteins was confirmed by SDS-PAGE to be used in an in vitro proliferation assay with bovine peripheral blood mononuclear cells (PBMCs). The ESP were exposed to the PBMCs during two different periods (24 and 48 h). Genes associated with immune response against the nematode were analyzed using relative gene expression and bioinformatic tools. These are simple, economic, and helpful tools to identify potential immune-protective molecules under in vitro conditions for confirming the efficacy of future in vivo assays. Graphical overview

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Analysis of the immunomodulatory activity of excreted and secreted products from Haemonchus placei transition infective larvae (xL3)

Cited by 4 publications

References 39 publications

In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs)

In vitro characterization of Haemonchus contortus trehalose-6-phosphate phosphatase and its immunomodulatory effects on peripheral blood mononuclear cells (PBMCs)

In silico analysis of two Haemonchus spp. serine protease peptides (S28) and their immunomodulatory activity in vitro

Selection of Molecules with Immunological Potential from Excretory and Secretory Products from the Nematode Haemonchus placei by Cell Proliferation and Gene Expression Assays

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