Analysis of the Interaction Between Stem Cells and Polylactic Acid (Pla) Scaffolds for Applications in Tissue Engineering

Biagini, G.; Marcon, BH; Pereira, Tarciso; Berti, LF; Senegaglia, AC; Correa, Alejandro; Stimamiglio, Marco Augusto

doi:10.1016/j.jcyt.2021.02.015

Cited by 4 publications

(4 citation statements)

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“…The percentage of cells in G0/G1, S, G2/M phase was calculated from the histogram plots. G0/G1 refers to the quiescent nondividing phase of cells, while S refers to the DNA synthesis phase and G2/M phase indicate the proliferating stage 46,47 . Cells cultured on CCF scaffolds shows the highest percentage of actively proliferating cells (55.6%) followed by CC (50.8%) and then C (47.6%).…”

Section: Resultsmentioning

confidence: 99%

“…G0/G1 refers to the quiescent nondividing phase of cells, while S refers to the DNA synthesis phase and G2/M phase indicate the proliferating stage. 46,47 Cells cultured on CCF scaffolds shows the highest percentage of actively proliferating cells (55.6%) followed by CC (50.8%) and then C (47.6%). Cells cultured on both CC and CCF scaffolds, indicate significant increase (p < .05) in dividing cells as compared with that of C scaffold, which has a higher percentage of cells at rest phase.…”

Section: Healing Progression Assessment In Vivomentioning

confidence: 98%

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Chitosan‐collagen‐fibrinogen uncrosslinked scaffolds possessing skin regeneration and vascularization potential

Dasgupta

Gope

Mukhopadhyay

et al. 2022

J Biomedical Materials Res

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Clinical success of regenerative medicine for treating deep‐tissue skin injuries depends on the availability of skin grafts. Though bioengineered constructs are tested clinically, lack of neovascularization provide only superficial healing. Thus constructs, which promotes wound healing and supports vascularization has gained priority in tissue engineering. In this study, chitosan‐collagen‐fibrinogen (CCF) scaffold was fabricated using freeze‐drying method without using any chemical crosslinkers. CCF scaffolds proved cytocompatibility and faster healing in in vitro scratch assay of primary human adult dermal fibroblasts cells with progressively increasing vascular endothelial growth factor‐A and reducing vascular endothelial growth factor receptor 1 expressions. Skin regeneration evaluated on in vivo full thickness wound model confirmed faster remodeling with angiogenic signatures in CCF scaffold‐implanted mice. Histopathological observations corroborated with stereo‐zoom and SS‐optical coherence tomography images of wound sites to prove the maturation of healing‐bed, after 12 days of CCF implantation. Therefore, it is concluded that CCF scaffolds are promising for skin tissue regeneration and demonstrates pro‐angiogenic potential.

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Section: Resultsmentioning

confidence: 99%

Section: Healing Progression Assessment In Vivomentioning

confidence: 98%

Chitosan‐collagen‐fibrinogen uncrosslinked scaffolds possessing skin regeneration and vascularization potential

Dasgupta

Gope

Mukhopadhyay

et al. 2022

J Biomedical Materials Res

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“…In particular, polymers have attracted special focus for designing biomaterial scaffolds due to their appropriate mechanical properties, fabrication versatility, and ability for multiple functionalization [8,[85][86][87]. Thus, either alone or in composites, a broad range of natural (e.g., chitosan [84,88,89], alginate [2,90], agarose [91], gelatin [92,93], hyaluronic acid [94,95], collagen [83,84,96,97]) and synthetic (e.g., PLGA [98][99][100], PLA [101,102], PEG [103], polycaprolactone (PCL) [104][105][106], polysialic acid (PSA) [106]) polymers have been investigated for creating cell-free and cell-seeded scaffolds for spinal cord regeneration.…”

Section: Biomaterials Scaffoldsmentioning

confidence: 99%

Novel Strategies for Spinal Cord Regeneration

Costăchescu

Niculescu

Dabija

et al. 2022

IJMS

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A spinal cord injury (SCI) is one of the most devastating lesions, as it can damage the continuity and conductivity of the central nervous system, resulting in complex pathophysiology. Encouraged by the advances in nanotechnology, stem cell biology, and materials science, researchers have proposed various interdisciplinary approaches for spinal cord regeneration. In this respect, the present review aims to explore the most recent developments in SCI treatment and spinal cord repair. Specifically, it briefly describes the characteristics of SCIs, followed by an extensive discussion on newly developed nanocarriers (e.g., metal-based, polymer-based, liposomes) for spinal cord delivery, relevant biomolecules (e.g., growth factors, exosomes) for SCI treatment, innovative cell therapies, and novel natural and synthetic biomaterial scaffolds for spinal cord regeneration.

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“…As a biodegradable material, polylactic acid (PLA) has good biocompatibility and degradability, which makes it widely used in medical material, 1,2 drug delivery, 3 and food packaging 4,5 . Recently, the production technology of PLA is becoming more and more mature 6 and many countries are actively improving the production capacity of PLA, 7–9 which provides a foundation for the large‐scale application of PLA.…”

Section: Introductionmentioning

confidence: 99%

Sustained antioxidant properties of epigallocatechin gallate loaded halloysite for PLA as potentially durable materials

Yao

Gong

et al. 2022

J of Applied Polymer Sci

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Halloysite nanotube (HNT) was etched by hydrochloric acid, and epigallocatechin gallate (EGCG) was loaded into the etched HNT (HH) to prepare loaded antioxidant (HH-E) for polylactic acid (PLA) with improved thermal-oxidative stability. The loading process was confirmed by TGA and FT-IR, and the loading capacity was 16.15%. The presence of EGCG on the surface of etched HNT was further confirmed by nitrogen adsorption and TEM. The effect of loaded EGCG on thermal-oxidative stability of PLA was characterized by the change in OOT values of PLA after the aging process under 140 C. The results showed that HH-E increased OOT values of PLA from 231.2 C to 293.6 C at first, indicating that HH-E provided PLA with excellent thermal-oxidative stability. Furthermore, as can be seen from the trend of OOT changing over aging time, during the 14 days aging process, OOT values of PLA composites with HH-E decreased more slowly than that of PLA composites with EGCG alone, endowing PLA with sustainable thermal-oxidative stability. Further kinetic analysis showed that the apparent activation energy of the degradation process (E a ) of PLA composites with HH-E changed less than that of PLA composites with EGCG alone, which showed that the loading system provided PLA better sustainable stability. Moreover, the isothermal crystallization process of modified PLA was characterized by DSC and POM. The results showed that HH-E provided heterogeneous crystal nucleus for PLA, reducing the half crystallization time (t 0.5 ) and improvement of crystallization rate for PLA. These results prove that HH-E exhibited excellent performance in improving the thermal stability and crystallization property of PLA, showing multifunctional characterization.

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Analysis of the Interaction Between Stem Cells and Polylactic Acid (Pla) Scaffolds for Applications in Tissue Engineering

Cited by 4 publications

References 0 publications

Chitosan‐collagen‐fibrinogen uncrosslinked scaffolds possessing skin regeneration and vascularization potential

Chitosan‐collagen‐fibrinogen uncrosslinked scaffolds possessing skin regeneration and vascularization potential

Novel Strategies for Spinal Cord Regeneration

Sustained antioxidant properties of epigallocatechin gallate loaded halloysite for PLA as potentially durable materials

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