Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines

Sk, Naess; Bradeen, James M.; Sm, Wielgus; Gt, Haberlach; Jm, McGrath; Jp, Helgeson

doi:10.1007/s004380100465

Cited by 43 publications

(15 citation statements)

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“…Understandably, most attention has been devoted to mapping resistance to late blight (Phytophthora infestans), the most important potato disease and responsible for the infamous Irish Potato Famine in 1845-46. The most remarkable source for resistance to P. infestans is S. bulbocastanum (Naess et al 2001;Lokossou et al 2010). Resistance to P. infestans was also mapped in Solanum demissum (Jo et al 2011), S. venturii (Pel et al 2009), S. pinnatisectum (Kuhl et al 2001), S. avilesii (Verzaux et al 2011), S. paucisectum (Villamon et al 2005) and in S. phureja x S. stenotomum (Costanzo et al 2005;Simko et al 2006).…”

Section: Mapping Toolsmentioning

confidence: 99%

Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

2018

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Potato is the third most important food crop in the world and is crucial to ensure food security. However, increasing biotic and abiotic stresses jeopardize its stable production. Fortunately, breeders count on a rich pool of wild relatives that provide sources for disease resistance and tolerance to environmental stresses. To use such traits effectively, breeders require tools that facilitate exploration and exploitation of the genetic diversity of potato wild relatives. Introgression programs to incorporate such alien chromatin into the crop have so far relied on cytogenetic and genetic studies to tap desired traits from these wild resources. The available genetic and cytogenetic tools, supplemented with more recent genomic technologies, can assist in the use of potato relatives in pre-breeding. This information can also facilitate cisgenesis and genome editing to improve potato cultivars. Despite the abundant and rapidly growing genomic information of potato, that of its wild relatives is still limited. Resumen La papa es el tercer alimento más importante en el mundo y es crucial para garantizar la seguridad alimentaria. No obstante, el aumento de los factores adversos bióticos y abióticos pone en riesgo la estabilidad de la producción. Afortunadamente, los mejoradores cuentan con rico acervo de parientes silvestres que suministran fuentes de resistencia a enfermedades y tolerancia a factores ambientales adversos. Para utilizar tales caracteres efectivamente, los mejoradores requieren de herramientas que faciliten la exploración y explotación de la diversidad genética de los parientes silvestres de la papa. Los programas de introgresión para incorporar tal cromatina ajena al cultivo hasta ahora han recurrido a estudios citogenéticos y genéticos para captar caracteres deseables de esas fuentes silvestres. Las herramientas genéticas y citogenéticas disponibles, suplementadas con tecnologías genómicas más recientes, pueden asistir en el uso de los parientes de la papa en pre-mejoramiento. Esta información también puede facilitar la cisgénesis y la edición genómica para mejorar las variedades de papa. A pesar del abundante y rápido crecimiento de la información genómica de la papa, la de los parientes silvestres es aún limitada.

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Section: Mapping Toolsmentioning

confidence: 99%

Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

2018

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“…Картирование и идентификация генов/QTLs, детерминирующих целевые признаки, и разработка тесно сцепленных с ними маркеров. В результате косегрегаци онного анализа ВС популяций, полученных в возвратных скрещиваниях межвидовых соматических гибридов, бы ли картированы гены S. bulbocastanum: RMc1 (XI), детер минирующий устойчивость к нематоде Meliodogyne chit woodi (Brown et al, 1995(Brown et al, , 1996 и RB (VIII), контролиру ющий нерасоспецифичную устойчивость к Phytophthora infestans (Naess et al, 2000(Naess et al, , 2001, а также ген Rlr etb (IV) неклубненосного вида S. etuberosum, который детермини рует устойчивость к ВСЛК (Novy et al, 2002…”

Section: Gene Pool and Breeding Vavilov Journal Of Genetics And Breedunclassified

Interspecific hybridization of potato: theoretical and applied aspects

Гавриленко¹,

Yermishin²

2017

Vestn. VOGiS

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The common potato Solanum tuberosum is the fourth most important crop in the world and like many other cultivated plants is characterized by low level of genetic diversity. The most effective approach to broaden genetic diversity of breeding varieties is based on the introgression of genetic material of wild and cultivated species belonging to section Petota Dumort. of the genus Solanum L. This section, according to estimates by different taxonomists, includes from 112 to 235 species. At the same time, because of the barriers of prezygotic and postzygotic incompatibility, only about 10 % of wild potato species have been actively involved into the breeding process and their genetic material has been introgressed into the gene pool of varieties. To date, molecular genetics, genomics, and biotechnology have accumulated a large amount of information, allowing the problems of extension of the genetic diversity of common potato to be addressed. The article presents a review of modern data about the genetic factors determining prezygotic and postzygotic incompatibility, about the features of involvement in the hybridization process of the self-compatible and self-incompatible species of section Petota and species with different values of the endosperm balance number (EBN), as well as possible mechanisms of interaction between the parental genomes during the formation of the endosperm of the hybrid seed. Data are provided on the value of the theory of EBN for the further development of applied research aimed at increasing the genetic diversity of the breeding gene pool. This review discusses the traditional and modern approaches and methods of overcoming interspecific incompatibility and gives examples of successful studies on introgressive hybridization of potato. The article is preceded by information about the genetic diversity of potato species belonging to section Petota of the genus Solanum.Key words: Solanum; potato; interspecific incompatibility; introgressive hybridization.Возделываемый картофель Solanum tuberosum L. является четвертой по значимости продовольственной культурой в мире и, как многие другие культурные растения, характеризуется низким уровнем генетического разнообразия. Наиболее эффективный подход к расширению генетического разнообразия селекционных сортов основан на интрогрессии генетического материала диких и культурных видов картофеля, относящихся к секции Petota Dumort. рода Solanum L., которая, по оценкам разных систематиков, включает от 112 до 235 видов. В то же время из-за барьеров презиготической и постзиготической несовместимости в генофонд селекционных сортов был интрогрессирован генетический материал лишь около 10 % дикорастущих видов картофеля. К на стоящему времени в области молекулярной генетики, геномики и биотехнологии накоплен большой объем информации, позволяющий решать задачи кардинального расширения генетического разнообразия культурных растений. В статье приведен краткий обзор современных данных о генетических факторах, детерминирующих презиготическую и постзиготическую несовмес...

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“…Recently, a single dominant late blight resistance gene was found in Solanum bulbocastanum Dun. and quantitative resistance genes were found in Solanum berthaultii Hawkes (Ewing et al, 2000;Naess et al, 2000Naess et al, , 2001.…”

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confidence: 99%

Characterization of Late Blight Resistance Derived from Solanum pimpinellifolium L3708 against Multiple Isolates of the Pathogen Phytophthora infestans

Kim¹,

Mutschler²

2006

jashs

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Sixteen tomato [Solanum lycopersicum L. (syn. Lycopersicon esculentum Mill.)] genotypes (inbred lines or hybrids) were tested against five Phytophthora infestans (Mont.) deBary isolates to characterize race specificity of late blight resistance transferred to tomato from Solanum pimpinellifolium L. [syn. Lycopersicon pimpinellifolium (L.) Mill.] accession L3708. The effects of plant genotype, isolate, genotype × isolate, and isolate × replication interactions were highly significant (P = 0.001). Set of four sister lines fixed for late blight resistance (CU-R lines) exhibited full and equal resistance to the five pathogen isolates tested. In contrast, the heterozygous F1 hybrids, created by crossing the resistant CU-R lines with a susceptible parent, were resistant to US-11; partially resistant to US-17, NC-1, and DR4B; and susceptible to US-7. Differential responses were also observed across pathogen isolates on a set of resistant sister lines (CLN-R lines), which also were bred from L3708. The CLN-R lines were resistant to the DR4B, NC-1, and US-11 isolates, but showed significant disease-affected areas and sporangium numbers following inoculation with either US-7 or US-17. Restriction fragment length polymorphism (RFLP) analysis confirms that both CU-R and CLN-R are homozygous for the Ph-3 gene derived from L3708. Since progeny tests also confirmed that the CLN-R lines are fixed for their level of resistance, these results suggest that late blight resistance in the CU-R lines is not controlled by Ph-3 alone, and that at least one additional gene conferring late blight resistance is missing from the CLN-R lines. Results of genetic tests of the (CU-R × CLN-R) F1 and a (CU-R × CLN-R) F2 population with the pathogen isolate US-17 strongly support a model in which resistance of the CU-R lines requires genes in addition to Ph-3. The implications of this information in breeding for late blight resistance and using of the resulting resistant lines or hybrids are discussed.

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Analysis of the introgression of Solanum bulbocastanum DNA into potato breeding lines

Cited by 43 publications

References 18 publications

Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

Introgressive Hybridization in Potato Revealed by Novel Cytogenetic and Genomic Technologies

Interspecific hybridization of potato: theoretical and applied aspects

Characterization of Late Blight Resistance Derived from Solanum pimpinellifolium L3708 against Multiple Isolates of the Pathogen Phytophthora infestans

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