Analysis of the lipid profile of saliva in ovarian and endometrial cancer by IR fourier spectroscopy

Bel’skaya, Lyudmila V.; Sarf, Elena A.; Solomatin, Denis V.; Kosenok, Victor K.

doi:10.1016/j.vibspec.2019.102944

Cited by 10 publications

(13 citation statements)

References 78 publications

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“…Unstimulated saliva (2 ml) was collected from each volunteer in the interval from 8 to 10 am in accordance with circadian rhythms. 35,36 Subjects rinsed their mouth with water 10–15 min before sampling. Saliva samples were centrifuged (10 000 × g for 10 min) to remove cell debris and reduce saliva turbidity.…”

Section: Methodsmentioning

confidence: 99%

Age and Gender Characteristics of the Infrared Spectra of Normal Human Saliva

2020

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The comparison of the characteristics of the infrared (IR) spectra of saliva of healthy volunteers was carried out based on gender and age. It is shown that statistically significant differences between male and female groups are observed for the absorption bands of proteins and lipids. At the same time, the absorbance of the bands assigned to proteins and nucleic acids is higher for males, whereas the absorbance of the bands assigned to lipids is higher in the group of females. It is established that the correlation relationships of the characteristics of the spectra and age are weakly expressed. Thus, when forming the criteria of the norm and pathology for saliva, it is necessary to take into account the gender of the subjects, while there are no strict requirements for taking into account age periodization. Nevertheless, the revealed patterns are valid only for the composition of the saliva of healthy volunteers, the extension of the results to groups of patients with various diseases, as well as other biological fluids, requires additional testing.

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Section: Methodsmentioning

confidence: 99%

Age and Gender Characteristics of the Infrared Spectra of Normal Human Saliva

2020

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“…Lipids from saliva samples were extracted using Folch solution (chloroform:ethanol = 2:1, vol.) [37,38]. When analyzing biological material, 200 µL of saliva was diluted with 800 µL of 0.9% NaCl, then the samples were extracted twice with 2 mL of Folch solution.…”

Section: Saliva Analysis By Ir Spectroscopymentioning

confidence: 99%

“…Previously, we simulated solutions with different lipid content and established absorption bands in the IR spectrum, the characteristics of which correlate with the level of lipids [37]. As analytical absorption bands, we selected the bands corresponding to the stretching (ν s -symmetric, ν as -asymmetric) and deformation (δ) vibrations of the methyl and methylene groups of lipids and fatty acids: 1396 cm −1 (δCH 3 ), 1458 cm −1 (δCH 2 ), 2853 cm −1 (ν s CH 3 ), 2923 cm −1 (ν as CH 2 ) and 2957 cm −1 (ν as CH 3 ) [37,38]. In parallel, we measured the intensity (H) and area (S) of the corresponding peaks in the IR spectra of the samples (Figure 1).…”

Section: Saliva Analysis By Ir Spectroscopymentioning

confidence: 99%

“…We have developed a method for the quantitative determination of lipids in saliva, in which, after extraction with a chloroform/ethanol mixture, lipids are determined by IR spectroscopy [37,38]. The bands corresponding to the stretching and deformation vibrations of the methyl and methylene groups of lipids and fatty acids were selected as analytical absorption bands: 1396, 1458, 2853, and 2923 cm −1 .…”

Section: Introductionmentioning

confidence: 99%

“…A multifactor regression model was constructed, which allows experimental data to be described with an error not exceeding 12%. On the example of ovarian and endometrial cancer, it was shown that in cancer, the lipid content in saliva decreases and a ratio of the intensities of absorption bands 2923/2957 cm −1 was proposed, the decrease in which is statistically significant both at the initial and at the advanced stages of the disease and can be used as a potential diagnostic criterion [38]. We also showed earlier that the composition of saliva has pronounced sex differences [39], which are also manifested in IR spectra [40].…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Saliva Lipids in Breast and Prostate Cancer by IR Spectroscopy

2021

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We have developed a method for studying the lipid profile of saliva, combining preliminary extraction and IR spectroscopic detection. The case–control study involved patients with a histologically verified diagnosis of breast and prostate cancer and healthy volunteers. The comparison group included patients with non-malignant pathologies of the breast (fibroadenomas) and prostate gland (prostatic intraepithelial neoplasia). Saliva was used as a material for biochemical studies. It has been shown that the lipid profile of saliva depends on gender, and for males it also depends on the age group. In cancer pathologies, the lipid profile changes significantly and also depends on gender and age characteristics. The ratio of 1458/1396 cm−1 for both breast and prostate cancer has a potential diagnostic value. In both cases, this ratio decreases compared to healthy controls. For prostate cancer, the ratio of 2923/2957 cm−1 is also potentially informative, which grows against the background of prostate pathologies. It is noted that, in all cases, changes in the proposed ratios are more pronounced in the early stages of diseases, which increases the relevance of their study in biomedical applications.

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