Analysis of the molecular size distribution of polyethylene glycols by CE‐UV <i>versus</i> HPLC with evaporative light scattering detection

Brinz, Daniela; Holzgrabe, Ulrike

doi:10.1002/elps.200800066

Cited by 12 publications

(6 citation statements)

References 13 publications

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“…The putative contaminated cell cultures need to undergo a rapid and sensitive detection procedure prior to collecting contaminated cells [5]. A potential problem is that Mycoplasma cells are physically small (less than 1 mm), which makes it difficult to detect them with a conventional microscope.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Kim¹,

Jung²,

Song³

et al. 2009

Electrophoresis

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We present real-time, rapid detection of Mycoplasma pneumonia in PBS inside a Y-channel PDMS microfluidic device via optical fiber monitoring of latex immunoagglutination. The latex immunoagglutination assay was performed with serially diluted M. pneumonia solutions using highly carboxylated polystyrene particles of 390 and 500 nm diameter conjugated with monoclonal anti-M. pneumonia. Proximity optical fibers were located around the viewing cell of the device, which were used to measure the increase in 45 degrees forward light scattering of the aggregated particles. The detection limit are slightly less than 50 pg mL(-1) both for 390 and 500 nm microspheres and the detection time do not exceed 90 s.

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Section: Introductionmentioning

confidence: 99%

Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Kim¹,

Jung²,

Song³

et al. 2009

Electrophoresis

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“…This method was able to separate PEGs up to an average molar mass of 1500 with acceptable resolution values. However, it failed to be applied to PEGs with higher chain lengths . Later, an efficient technique was developed using a RP-HPLC gradient performed on a C18 column with a binary gradient of acetonitrile and water and a nebulization chamber at 30 °C.…”

Section: Characterizationmentioning

confidence: 99%

Striving for Uniformity: A Review on Advances and Challenges To Achieve Uniform Polyethylene Glycol

Bento,

Katz,

Santos

et al. 2024

Org. Process Res. Dev.

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Poly(ethylene glycol) (PEG) is the polymer of choice in drug delivery systems due to its biocompatibility and hydrophilicity. For over 20 years, this polymer has been widely used in the drug delivery of small drugs, proteins, oligonucleotides, and liposomes, improving the stability and pharmacokinetics of many drugs. However, despite the extensive clinical experience with PEG, concerns have emerged related to its use. These include hypersensitivity, purity, and nonbiodegradability. Moreover, conventional PEG is a mixture of polymers that can complicate drug synthesis and purification leading to unwanted immunogenic reactions. Studies have shown that uniform PEGylated drugs may be more effective than conventional PEGylated drugs as they can overcome issues related to molecular heterogeneity and immunogenicity. This has led to significant research efforts to develop synthetic procedures to produce uniform PEGs (monodisperse PEGs). As a result, iterative step-by-step controlled synthesis methods have been created over time and have shown promising results. Nonetheless, these procedures have presented numerous challenges due to their iterative nature and the requirement for multiple purification steps, resulting in increased costs and time consumption. Despite these challenges, the synthetic procedures went through several improvements. This review summarizes and discusses recent advances in the synthesis of uniform PEGs and its derivatives with a focus on overall yields, scalability, and purity of the polymers. Additionally, the available characterization methods for assessing polymer monodispersity are discussed as well as uniform PEG applications, side effects, and possible alternative polymers that can overcome the drawbacks.

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“…Brinz and Holzgrabe used free zone CE to assess the size distribution of polyethylene glycols (213). PEGs were derivatized with phthalic anhydride using a microwave assisted reaction.…”

Section: Applicationsmentioning

confidence: 99%

Capillary Electrophoresis

2010

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Analysis of the molecular size distribution of polyethylene glycols by CE‐UV versus HPLC with evaporative light scattering detection

Cited by 12 publications

References 13 publications

Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Rapid detection of Mycoplasma pneumonia in a microfluidic device using immunoagglutination assay and static light scattering

Striving for Uniformity: A Review on Advances and Challenges To Achieve Uniform Polyethylene Glycol

Capillary Electrophoresis

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