Analysis of the molecular structure of human enamel with fluorosis using micro-Raman spectroscopy

Abstract

) were obtained to analyze structural differences among the specimens. Although the differences were not statistically significant (P > 0.05), the mean of integral areas of ν 1 phosphate peak among groups indicated greater mineralization in the severe fluorosis group. However, there were statistically significant differences in the intensities, and the integral areas of B-type carbonate peak among groups (P < 0.05). Therefore, mineralization of the carbonate peak at 1070 cm −1 decreased significantly in fluorotic groups, suggesting that carbonate ions are easily dissolved in the presence of fluoride. Although structurally fluorotic teeth are not more susceptible to dental caries, serious alteration in its surface topography may cause retention of bacterial plaque and formation of enamel caries. Micro-Raman spectroscopy is a useful tool for analyzing the molecular structure of healthy and fluorotic human enamel. (J Oral Sci 54, 93-98, 2012)

Applications of Raman Spectroscopy in Dentistry: Analysis of Tooth Structure

“…Raman spectroscopy helps in characterizing hydroxyapatite crystals (Ca 10 (PO 4 ) 6 (OH) 2 (3,36,39). Several studies strongly recommend Raman spectroscopy as an effective and relatively easy tool for early diagnosis of dental caries (32,(40)(41)(42)(43)(44)(45)(46)(47)(48)(49)(50) because during the caries process the inorganic matter of the tooth is gradually replaced by organic matter, which shows stronger organic peaks than normally seen. These changes are characterized by studying material compositions by exciting chemical bonds in the specimen (51).…”

“…Zavala-Alonso et al (49) studied the molecular structure of human enamel affected by fluorosis. They studied mild, moderate, and severe cases of fluorosis using micro-Raman spectroscopy and compared the structural difference in phosphate (960 cm −1 ) and B-type carbonate (1070 cm −1 ) peaks with healthy enamel.…”

Phase Transformations in a Human Tooth Tissue at the Initial Stage of Caries

“…Micro-Raman was used to characterize dental lesions chemically in this investigation. The study of chemical composition of tooth tissue structures is important in biomedical materials because of the sensitivity of biological systems to the local atomic order [ 15 ].…”

Chemical imaging of human teeth by a time-resolved Raman spectrometer based on a CMOS single-photon avalanche diode line sensor

“…In dentistry, Raman spectroscopy has been used in the early detection and characterization of dental caries, analysis of fluorosis-affected teeth and chemical profiling of adhesive/caries-affected dentin interface, for example. [11][12][13][14][15] Raman spectroscopy have major advantages over other optical spectroscopy techniques, such as IR spectro-scopy, when considering biological specimens. These include minimal sample preparation and the weak Raman interference with water.…”