Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

Zhang, Jingjing; Zhang, Quanbin; Wang, Jing; Shi, Xuelian; Zhang, Zhongshan

doi:10.1007/s00343-009-9205-0

Cited by 95 publications

(50 citation statements)

References 6 publications

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“…For the determination of the sugar composition, the acid-hydrolyzed glycoses were converted to the corresponding 1-phenyl-3-methyl-5-pyrazolone derivatives (PMP) and separated by HPLC chromatography (Zhang et al, 2009;Jin et al, 2013). The molecular weights of the samples were determined by gel permeation chromatography (GPC) on a TSK gel PWxl 3000 column (7.8 mm×300 mm, 7 μm), which was eluted with 0.2 mol/L Na 2 SO 4 at a fl ow rate of 0.5 mL/min at 30°C .…”

Section: Composition Methodsmentioning

confidence: 99%

Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Zhang

Sun²,

Zhao

et al. 2015

Chin. J. Ocean. Limnol.

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A rapid, sensitive and reproducible high performance liquid chromatography (HPLC) method with post-column fl uorescence derivatization has been developed to determine the amount of low-molecularweight sulfated polysaccharide (GFS) in vivo . The metabolism of GFS has been shown to fi t a two component model following its administration by intravenous injection, and its pharmacokinetic parameters were determined to be as follows: half-time of distribution phase ( t 1/2α )=11.24±2.93 min, half-time of elimination phase ( t 1/2β )=98.20±25.78 min, maximum concentration ( C max )=110.53 μg/mL and peak time ( T max )=5 min. The pharmacokinetic behavior of GFS was also investigated following intragastric administration. However, the concentration of GFS found in serum was too low for detection, and GFS could only be detected for up to 2 h after intragastric administration (200 mg/kg body weight). Thus, the bioavailability of GFS was low following intragastric administration because of the metabolism of GFS. In conclusion, HPLC with postcolumn derivatization could be used for quantitative microanalysis and pharmacokinetic studies to determine the presence of polysaccharides in the serum following intravenous injection.

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Section: Composition Methodsmentioning

confidence: 99%

Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Zhang

Sun²,

Zhao

et al. 2015

Chin. J. Ocean. Limnol.

Self Cite

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“…Among them, catalytic decomposition is the most widely used method, due to its advantages of mild reaction conditions, high efficiency and low cost. Numerous catalysts have been investigated, including transition metal oxides [14][15][16][17][18][19][20][21], activated carbon [22,23] and noble metals [24][25][26]. Among these catalysts, manganese oxides (MnO x ) usually show good catalytic performance in ozone decomposition.…”

Section: Introductionmentioning

confidence: 99%

Decomposition of high-level ozone under high humidity over Mn–Fe catalyst: The influence of iron precursors

Lian

2015

Catalysis Communications

114

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a b s t r a c t a r t i c l e i n f oManganese-iron mixed oxide, an efficient and stable catalyst, has been successfully used in the decomposition of ozone. The influence of different iron precursors on the catalytic decomposition activity of high-level ozone under high-humidity over manganese-iron catalysts prepared using a hydrothermal approach was studied. The catalytic performance over MnFe 0.5 O x -Fe(NO 3 ) 3 was much better than that of MnFe 0.5 O x -FeSO 4 and MnFe 0.5 O x -FeCl 3 . From the results of characterization by N 2 physical adsorption, XRD, XPS and SEM, it was concluded that the largest specific surface area, the lowest crystallinity, the most evenly distributed particle size and the most surface-active Mn 2+ and Mn 3+ led to the best catalytic activity for the MnFe 0.5 O x -Fe(NO 3 ) 3 catalyst.

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“…The hydrolysis of polysaccharides was performed using previously described methods [17]. PRP (20 mg) was hydrolyzed in TFA (2 M, 5 mL) for 5 h at 110 °C in a sealed test tube.…”

Section: Monosaccharide Composition Analysismentioning

confidence: 99%

Extraction optimization and characterization of polysaccharide antioxidants from <i>Pinellia ternata</i> (Thunb) Breit rhizome

Mo¹,

Liu²,

Tao³

et al. 2017

Trop. J. Pharm Res

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Analysis of the monosaccharide composition of fucoidan by precolumn derivation HPLC

Cited by 95 publications

References 6 publications

Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Microanalysis and preliminary pharmacokinetic studies of a sulfated polysaccharide from Laminaria japonica

Decomposition of high-level ozone under high humidity over Mn–Fe catalyst: The influence of iron precursors

Extraction optimization and characterization of polysaccharide antioxidants from <i>Pinellia ternata</i> (Thunb) Breit rhizome

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