Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae

Inanime, Julia M.; Loechel, Steve; Hu, Ping-Chuan

doi:10.1016/0378-1119(88)90323-x

Cited by 75 publications

(66 citation statements)

References 19 publications

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“…If this were so then only a few nucleotides need be expected between the stop codons and start codons of consecutive coding sequences. In this respect the pMGA system of M. gallisepticum differs substantially from the Pl system of the phylogenetically related pathogen M. pneumoniae in which the Pl gene is encoded by a polystronic RNA which encodes two additional polypeptides [5]. Recent Northern blot experiments from this laboratory have confirmed that the length of pMGA mRNA is consistent with a monocistronic RNA (M.D.…”

Section: ------Aagmgtpcttaggag?tctgggcitttmentioning

confidence: 98%

The organisation of the multigene family which encodes the major cell surface protein, pMGA, of Mycoplasma gallisepticum

et al. 1994

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The genome of the avian pathogen Mycoplasma galhsepticum contains a number of related genes for putative adhesion molecules @MGA). Cloning and sequence analysis of several pMGA genes suggested that all of them might be transcriptionally and translationally functional. Analysis of the gene sequence encoding the sole pMGA variant expressed in vitro in the S6 strain (pMGAl.1) revealed no unambiguous feature that could account for its unique expression. It is estimated that the pMGA gene family may contain up to 50 members, and its possible role is discussed herein.

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Section: ------Aagmgtpcttaggag?tctgggcitttmentioning

confidence: 98%

The organisation of the multigene family which encodes the major cell surface protein, pMGA, of Mycoplasma gallisepticum

et al. 1994

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“…Genetic differences of M. pneumoniae patient isolates were first described by Su et al (1990). They used the gene P1 (MPN141), which encodes the main adhesin P1 (Inamine et al, 1988;Su et al, 1987) as a discriminating probe. Based on sequence differences of the P1 gene and restriction fragment length polymorphism of genomic DNA, the species M. pneumoniae was divided into two subtypes.…”

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confidence: 99%

Cross-complementation between the products of the genes P1 and ORF6 of Mycoplasma pneumoniae subtypes 1 and 2

Catrein

Dumke²,

Weiner

et al. 2004

Microbiology

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The genes P1 (MPN141) and ORF6 (MPN142) are essential for the successful colonization of the human respiratory tract by Mycoplasma pneumoniae. Both genes are located in the P1 operon, which consists of three genes. The P1 gene is the second gene in the operon, followed by the ORF6 gene. The P1 gene contains two (RepMP2/3, RepMP4) and the ORF6 gene one (RepMP5) specific repetitive DNA sequence, of which seven to nine similar but not identical copies are dispersed on the genome. Despite this large potential pool for genetic variation, M. pneumoniae isolates from patients contain only one of two distinct combinations of the genes P1 and ORF6. To analyse the functions of the repetitive DNA sequences, two 'new' combinations of the genes P1 and ORF6 were constructed, keeping the P1 gene constant but exchanging RepMP5 copies of the ORF6 gene. M. pneumoniae was transformed with these constructs and the transformants were tested for their ability to grow and survive under in vitro conditions and in guinea pigs. The two transformants colonized the respiratory tract of guinea pigs and showed no obvious differences in their growth behaviour compared to M. pneumoniae isolates from patients. The results indicate that the subtype-specific combinations of the repetitive elements in the P1 and ORF6 genes are not essential for the successful adherence of M. pneumoniae to host cells and the colonization of the respiratory tract of guinea pigs.

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“…If this structure is not properly asserhbled-if, for instance, the P1 protein is missing-then the bacterium is avirulent. The P1 protein is coded for by the P1 gene (13, 35), which is part of the Pl operon (14). According to DNA sequencing data, the P1 operon consists of three open reading frames, ORF4 and ORF6 flanking the Pl gene (ORF5).…”

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confidence: 99%

Sequence divergence in the ORF6 gene of Mycoplasma pneumonia

1994

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The OR1F6 gene product of Mycopklsma pneumonae is involved in a yet-unknown manner in the adhesion of the bacterium to its host cell. Part of the ORF6 gene is a repetitive DNA sequence (RepMP5), about 1,900 bp Iong. Seven additiohal similar copies of RcpMPS are dispersed on the genome. In the independently isolated strains M. pneumoniae M129 and FH, the RepMP5 .opies residing in the ORF6 gene are not identical. Two conserved regions, ranging from nucleotides 1 to 799 and from nucleotide 1795 to the end-of the gene, border a vairiable region, ran,lng from nucleotides 800 to 1794. This variable region. differs in DNA sequence and by 201 bp. Analysis,of R'pMP5 copies outside the ORF6 geine shewed that both M. pneumoniae M129 and M.pneumoniae FH carry a RepMP5 copy on a 6-kbp EcoRI fragment which has the same DNA sequence as the yariable region of RepMP5 in the M. pneumoniae FH ORF6 gene. According to these data, a switch from the M. pneumoniae M129 bRF6 gene to the M. pneumoniae FH ORF6 gene could take place by gene conversion.The bacterium Mycoplasma pneumoniae is the causative agent of a primary atypical pneumonia in humans (16,36). As a surface parasite, it has to ensure a firm interaction with its host cell and to find ways to evade the immune response of the host. The mecharlisms M. pneumoniae and other mycoplasma species use to cope with the immune system of the host are still not understood, but it is well documented that M. pneumoniae persists in humans, even after successful chemothhrApy (15).The prerequisite for persistence is the ability of these bacteria to adhere to their host cells (22). Adherence of M. pneumoniae is mediated by adhesins which bind to the host receptor. They are still ill-defined. There is more than one type of receptor.Sialoglycoproteins (19,20,23), sulfated glycolipids.(17), or a sialic acid-free glycoprotein (10) have been identified as receptors or as components of receptors.The major bacterial adhesin is the 170-kDa protein P1 (11,12). In addition, a 30-kDa protein has been sdggested to be involved in adherence (5). Both proteins are part of a tip-like organelle (2, 7). If this structure is not properly asserhbled-if, for instance, the P1 protein is missing-then the bacterium is avirulent. The P1 protein is coded for by the P1 gene (13, 35), which is part of the Pl operon (14). According to DNA sequencing data, the P1 operon consists of three open reading frames, ORF4 and ORF6 flanking the Pl gene (ORF5 are located in the P1 gene (RepMP2/3 and RepMP4) (26,32,38), and a third one (RepMP5) is located in the ORF6 gene (see Fig. 1) (4, 26). Su et al. (33,34) classified the species M. pneumoniae into two groups (I and II) based upon different hybridization patterns of the Pl gene carrying DNA fragments in Southern blots of restricted genomic DNA of independently isolated strains. The prototype of group I is M. pn"eutnoniae M129, and that of group II is M. pneumoniae FH.DNA sequence comparison between the P1 gene of M.pneumoniae FH and that of M. pneumoniae M129 showed differences at t...

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Analysis of the nucleotide sequence of the P1 operon of Mycoplasma pneumoniae

Cited by 75 publications

References 19 publications

The organisation of the multigene family which encodes the major cell surface protein, pMGA, of Mycoplasma gallisepticum

The organisation of the multigene family which encodes the major cell surface protein, pMGA, of Mycoplasma gallisepticum

Cross-complementation between the products of the genes P1 and ORF6 of Mycoplasma pneumoniae subtypes 1 and 2

Sequence divergence in the ORF6 gene of Mycoplasma pneumonia

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