Analysis of the plasmid content of three Î²-lactamase-producing<i>Neisseria gonorrhoeae</i>strains

Fayet, Olivier; Froment, Yvette; Piffaretti, Jean-Claude

doi:10.1111/j.1574-6968.1982.tb00012.x

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Bacterial Strains and Bacteriophage Divas2

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1986

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“…The N. gonorrhoeae strain used in this study, GX, is a clinical isolate. The media and growth conditions have been described previously [7].…”

Section: Bacterial Strains and Bacteriophage Divasmentioning

confidence: 99%

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DNA methylase activities in aNeisseria gonorrhoeaeextract

Prère¹,

Fayet²

1986

FEMS Microbiology Letters

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Resistance of Neisseria gonorrhoeae DNA to cleavage by various restriction endonucleases suggests the existence of modification enzymes which protect the corresponding recognition sequences. We indeed found methylase activities in N. gonorrhoeae extracts. These activities lead to the methylation of adenine and cytosine residues in bacteriophage λ DNA and DNA from an Escherichia coli Dam− strain. They also result in partial protection of λ DNA to cleavage by the restriction endonucleases HaeII, HaeIII, BamHI and, SacII.

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“…The N. gonorrhoeae strain used in this study, GX, is a clinical isolate. The media and growth conditions have been described previously [7].…”

Section: Bacterial Strains and Bacteriophage Divasmentioning

confidence: 99%

“…coli strain GM99 (F-, dam4 ma1354, tsx, L. Caro collection) was used as a source of methyl-adenine-free DNA. Chromosomal DNA was purified as described previously [7].…”

Section: Bacterial Strains and Bacteriophage Divasmentioning

confidence: 99%