Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8

MacHugh, Niall D.; Bensaid, Albert; Howard, Chris; Davis, William C.; Morrison, William J.

doi:10.1016/0165-2427(91)90096-u

Cited by 40 publications

(19 citation statements)

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“…Additionally, there are differences in the type of CD8 molecule found on ␥␦ T cells from adult humans vs those in newborn ruminants, with respect to the type of CD8 molecule they express, which likely impacts these results. In humans, many CD8 ϩ ␥␦ T cells express the CD8␣␣ homodimer (41); whereas, most circulating CD8 ϩ ␥␦ T cells in young cattle express the CD8␣␤ heterodimer (42). Thus, if a human correlate to the results reported in this study exists, it may reside in a subset of the overall CD8 ϩ pool of ␥␦ T cells (those that express the CD8␣␤ heterodimer, for example) or at different stages of development (i.e., in neonates).…”

Section: Discussionmentioning

confidence: 99%

Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

Wilson

Hedges

Butcher

et al. 2002

The Journal of Immunology

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Subsets of γδ T cells localize to distinct tissue sites in the absence of exogenous Ag stimulation or development of effector/memory cells. Selective lymphocyte homing from the blood into tissues is controlled by a multistep process involving vascular and lymphocyte adhesion molecules, and G protein-linked chemokine receptors. The role of these mechanisms in the tissue tropism of γδ T cells is still poorly understood. In this study, we demonstrate that a subset of γδ T cells, most of which express an antigenically distinct TCR and are characterized by coexpression of CD8, selectively accumulated in tissues that expressed high levels of the mucosal vascular addressin, mucosal addressin cell adhesion molecule 1. These cells expressed higher levels of α4β7 integrins than other γδ T cell subsets and selectively migrated to the CCR7 ligand secondary lymphoid-tissue chemokine (CCL21). Integrin activation by CCL21 selectively increased CD8+γδ T cell binding to recombinant mucosal addressin cell adhesion molecule 1. These results suggest that the tropism of circulating CD8+γδ T cells for mucosal tissues is due, at least in part, to selective developmental expression of adhesion molecules and chemokine receptors.

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Section: Discussionmentioning

confidence: 99%

Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

Wilson

Hedges

Butcher

et al. 2002

The Journal of Immunology

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“…Lymphocyte-subset purities were monitored by using the following mAbs: CC8 (IgG2a, anti-bovine CD4) (Howard et al, 1989;Morrison et al, 1994); CC58 (IgG1, anti-bovine CD8 a/b) (MacHugh et al, 1991); CC63 (IgG2a, anti-bovine CD8 a/b and a/a chain) (MacHugh et al, 1991); CC15 (IgG2a, anti-bovine WC1) (Fikri et al, 2000); and GB21A (IgG2b, anti-bovine cd T-cell receptor) (Davis et al, 1996). The purity of the CD8 + T cells was tested by flow cytometry prior to each assay and .95 % of the cells separated by MACS were found to be CD8 + ab T cells.…”

Section: Methodsmentioning

confidence: 99%

An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV

Guzman¹,

Taylor²,

Charleston³

et al. 2008

Journal of General Virology

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Foot-and-mouth disease virus (FMDV) causes a highly contagious disease of cloven-hooved animals that carries enormous economic consequences. CD8 + cytotoxic T lymphocytes play an important role in protection and disease outcome in viral infections but, to date, the role of the CD8 + T-cell immune response to FMDV remains unclear. This study aimed to investigate major histocompatibility complex (MHC) class I-restricted CD8 + T-cell responses to FMDV in vaccinated and in infected cattle. An in vitro assay was used to detect antigen-specific gamma interferon release by CD8 + T cells in FMDV-infected cattle of known MHC class I genotypes.A significant MHC class I-restricted CD8 + T-cell response was detected to both FMDV strain O1 BFS and a recombinant fowlpox virus expressing the structural proteins of FMDV. Antigen-specific MHC class I-restricted CD8 + T-cell responses were also detected in cattle vaccinated with inactivated FMDV. These responses were shown to be directed, at least in part, to epitopes within the structural proteins (P12A region) of the virus. By using mouse cells expressing single cattle MHC class I alleles, it was possible to identify the restriction elements in each case. Identification of these epitopes will facilitate the quantitative and qualitative analysis of FMDV-specific memory CD8 + T cells in cattle and help to ensure that potential vaccines induce a qualitatively appropriate CD8 + T-cell response.

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“…The strain of M. bovis used (T/91/1378) was isolated originally in Northern Ireland from a field case of bovine tuberculosis. Blood samples were taken from each animal on the following days: Ϫ1, 0, 1,4,7,11,14,19,21,25,28,32,35, and 39. PBMC were isolated and used on each day both for assessment of cell-mediated immune functions (including IFN-␥ production) and for FCA.…”

Section: Methodsmentioning

confidence: 99%

Modulation of Immune Responses toMycobacterium bovisin Cattle Depleted of WC1⁺γδ T Cells

Kennedy

Welsh²,

Bryson³

et al. 2002

Infect Immun

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؉ -depleted animals exhibited decreased antigen-specific lymphocyte proliferative response, an increased antigen-specific production of interleukin-4, and a lack of specific immunoglobulin G2 antibody. This suggests that WC1 ؉ ␥␦ TCR ؉ cells contribute, either directly or indirectly, toward the Th1 bias of the immune response in bovine tuberculosis-a hypothesis supported by the decreased innate production of IFN-␥, which was observed in WC1 ؉ -depleted calves.

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Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8

Cited by 40 publications

References 1 publication

Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV

Modulation of Immune Responses toMycobacterium bovisin Cattle Depleted of WC1⁺γδ T Cells

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Analysis of the reactivity of anti-bovine CD8 monoclonal antibodies with cloned T cell lines and mouse L-cells transfected with bovine CD8

Cited by 40 publications

References 1 publication

Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

An MHC-restricted CD8+ T-cell response is induced in cattle by foot-and-mouth disease virus (FMDV) infection and also following vaccination with inactivated FMDV

Modulation of Immune Responses toMycobacterium bovisin Cattle Depleted of WC1+γδ T Cells

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Modulation of Immune Responses toMycobacterium bovisin Cattle Depleted of WC1⁺γδ T Cells