Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

Wilson, Eric; Hedges, Jodi F.; Butcher, Eugene C.; Briskin, Michael; Jutila, Mark A.

doi:10.4049/jimmunol.169.9.4970

Cited by 53 publications

(39 citation statements)

References 42 publications

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“…The gd T cells were the main recirculating subset in both afferent and efferent lymph, able to traffic from the blood through the skin back into the afferent and efferent lymph within 4 h. Both CD4 and CD8 T cells require CCR7 expression for their egress from resting extralymphoid tissues into draining LNs via the afferent lymph (16,17), and lymphatic endothelial cells of the afferent lymphatics constitutively express the CCR7 ligand CCL21 (13)(14)(15). CCL21 responsiveness in chemotaxis assays has been described for a subset of CD8 + gd T cells previously (27). CCR7 transcript expression in WC1 + gd T cell subsets, however, was found to be low by others (28).…”

Section: Discussionmentioning

confidence: 99%

γδ T Cell Homing to Skin and Migration to Skin-Draining Lymph Nodes Is CCR7 Independent

Vrieling

Santema

Rhijn

et al. 2012

The Journal of Immunology

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In most species, γδ T cells preferentially reside in epithelial tissues like the skin. Lymph duct cannulation experiments in cattle revealed that bovine dermal γδ T cells are able to migrate from the skin to the draining lymph nodes via the afferent lymph. For αβ T cells, it is generally accepted that epithelial and mucosal tissue egress is regulated by expression of the CCR7 chemokine receptor. In this study, we tracked the migratory route of bovine lymph-derived γδ T cells and examined their CCR7 cell surface expression in several compartments along this route. Total lymph cells from afferent and efferent origin were labeled with PKH fluorescent dyes and injected into the bloodstream. PKH+ cells already reappeared in the afferent lymph after 4 h. The vast majority of the PKH+ cells retrieved from the afferent lymph were of the WC1+ γδ T cell phenotype, proving that this PKH+ γδ T cell subset is able to home to and subsequently exit the skin. PKH+ γδ T cells from afferent and efferent lymph lack CCR7 surface expression and display high levels of CD62L compared with CD4 T cells, which do express CCR7. Skin homing receptors CCR4 and CCR10 in contrast were transcribed by both CD4 and γδ T cells. Our findings suggest that γδ T cell skin egress and migration into the peripheral lymphatics is CCR7-independent and possibly mediated by CD62L expression.

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Section: Discussionmentioning

confidence: 99%

γδ T Cell Homing to Skin and Migration to Skin-Draining Lymph Nodes Is CCR7 Independent

Vrieling

Santema

Rhijn

et al. 2012

The Journal of Immunology

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“…However, in all cases (n ϭ 8), Nanog T cells in the periphery also express integrin ␣4␤7, which is not detected in Nanog T cells in the cortex during the thymus atrophy phase ( Figure 6C). Integrin ␣4␤7 (LPAM-1) expressed in a subset of effector T cells or ␥␦T cells in the intestine 31,32 can mediate cell migration as a receptor for MAdCAM-1 and VCAM-1. [33][34][35] Therefore, the expression of integrin ␤7 in Nanog T cells BLOOD, 1 JULY 2007 ⅐ VOLUME 110, NUMBER 1 For personal use only.…”

Section: Discussionmentioning

confidence: 99%

Forced expression of Nanog in hematopoietic stem cells results in a γδT-cell disorder

Tanaka¹,

Era²,

Nishikawa

et al. 2007

Blood

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Nanog is a key molecule involved in the maintenance of the self-renewal of undifferentiated embryonic stem (ES) cells. In this work we investigate whether Nanog can enhance self-renewal in hematopoietic stem cells. Contrary to our expectation, no positive effect of Nanog transduction was detected in bone marrow reconstitution assays. However, recipients of Nanog-transduced (Nanog) hematopoietic stem cells (HSCs) invariantly develop a unique disorder typified by an atrophic thymus occupied by Nanogexpressing ␥␦T-cell receptor-positive (TCR ؉ ) cells (Nanog T cells). All thymi are eventually occupied by Nanog T cells with CD25 ؉ CD44 ؉ surface phenotype that home selectively to the thymus on transfer and suppress normal thymocyte development, which is partly ascribed to destruction of the microenvironment in the thymus cortex. Moreover, this initial disorder invariantly develops to a lymphoproliferative disorder, in which Nanog T cells undergo unlimited proliferation in the peripheral lymphoid tissues and eventually kill the host. This invariable end result suggests that Nanog is a candidate oncogene for ␥␦T-cell IntroductionThe self-renewal capacity and multipotency of embryonic stem (ES) cells can easily be maintained in culture. Several molecules have been implicated in the maintenance of these essential features of ES cells. Oct3/4 in association with the high mobility group (HMG) factor Sox2 is reported to be essential for self-renewal of ES cells, 1-3 and forced Nanog expression can maintain the immature state of ES cells in a LIF-Stat3-independent manner. 4,5 However, the exact molecular mechanism underlying the maintenance of the stem cell state is yet to be elucidated.Several reports have suggested that the core factors maintaining ES self-renewal can affect the activity of other stem cells. For example, Oct3/4 is expressed in mesenchymal stem cells derived from CD133 ϩ cells in mobilized peripheral blood and cord blood. 6 Nanog was also shown to be expressed in circulating CD14 ϩ CD34 low endothelial cells. 7 Although the role of these molecules in adult tissue stem cells remains unclear, it has been expected that it may be possible to use them to enhance the self-renewal of tissue stem cells. This expectation is supported by the recent report of Hochedlinger et al 8 showing that Oct3/4 overexpression enhances the proliferation of tissue stem cells.Compared with ES cells, it has been difficult to maintain the self-renewal of unmanipulated hematopoietic stem cells in vitro, although forced expression of HoxB4 or activated ␤-catenin confers enhanced self-renewal to hematopoietic progenitor cells (HPCs) in vitro. 9,10 Hence, it is worthwhile to determine whether the molecules involved in the self-renewal of ES cells can enhance that of HPCs. The initial aim of this work was to evaluate the effect of ectopic expression of Nanog on the behavior of hematopoietic stem cells. Although this expectation was not the case, we report here that Nanog overexpression induces an unusual ␥␦T-cell receptor-positive (TCR...

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“…TCR cells were CD2 ϩ and Ͼ90% CD8 ϩ , and the GD3.5 ϩ cells lacked CD2 and CD8 (data not shown) (8,16,20,29). Because of our past (16) and current focus on CD8, we refer to these subsets as being either CD8 ϩ or CD8 Ϫ ␥␦ T cells.…”

Section: High Speed Cell Sorting Of ␥␦ T Cell Subsets and Sage Librarmentioning

confidence: 99%

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

Meissner

Radke

Hedges

et al. 2003

The Journal of Immunology

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Gene expression profiles were compared in circulating bovine GD3.5+ (CD8−) and GD3.5− (predominantly CD8+) γδ T cells using serial analysis of gene expression (SAGE). Approximately 20,000 SAGE tags were generated from each library. A comparison of the two libraries demonstrated 297 and 173 tags representing genes with 5-fold differential expression in GD3.5+ and GD3.5− γδ T cells, respectively. Consistent with their localization into sites of inflammation, GD3.5+ γδ T cells appeared transcriptionally and translationally more active than GD3.5− γδ cells. GD3.5− γδ T cells demonstrated higher expression of the cell proliferation inhibitor BAP 37, which was associated with their less activated gene expression phenotype. The immune regulatory and apoptosis-inducing molecule, galectin-1, was identified as a highly abundant molecule and was higher in GD3.5+γδ T cells. Surface molecules attributed to myeloid cells, such as CD14, CD68, and scavenger receptor-1, were identified in both populations. Furthermore, expression of B lymphocyte-induced maturation protein, a master regulator of B cell and myeloid cell differentiation, was identified by SAGE analysis and was confirmed at the RNA level to be selectively expressed in γδ T cells vs αβ T cells. These results provide new insights into the inherent differences between circulating γδ T cell subsets.

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Bovine γδ T Cell Subsets Express Distinct Patterns of Chemokine Responsiveness and Adhesion Molecules: A Mechanism for Tissue-Specific γδ T Cell Subset Accumulation

Cited by 53 publications

References 42 publications

γδ T Cell Homing to Skin and Migration to Skin-Draining Lymph Nodes Is CCR7 Independent

γδ T Cell Homing to Skin and Migration to Skin-Draining Lymph Nodes Is CCR7 Independent

Forced expression of Nanog in hematopoietic stem cells results in a γδT-cell disorder

Serial Analysis of Gene Expression in Circulating γδ T Cell Subsets Defines Distinct Immunoregulatory Phenotypes and Unexpected Gene Expression Profiles

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