Analysis of the Roles of the ISLR2 Gene in Regulating the Toxicity of Zearalenone Exposure in Porcine Intestinal Epithelial Cells

Abstract

Zearalenone (ZEN) is one of the mycotoxins that pose high risks for human and animal health, as well as food safety. However, the regulators involved in ZEN cellular toxicity remain largely unknown. Herein, we showed that cell viability of porcine intestinal epithelial cells (IPEC-J2) tended to decrease with increasing doses of ZEN by the cell counting kit-8 assay. Expression of the ISLR2 (immunoglobulin superfamily containing leucine-rich repeat 2) gene in IPEC-J2 cells was significantly downregulated upon ZEN exposure. Furthermore, we found the dose–effect of ZEN on ISLR2 expression. We then overexpressed the ISLR2 gene and observed that overexpression of ISLR2 obviously reduced the effects of ZEN on cell viability, apoptosis rate and oxidative stress level. In addition, ISLR2 overexpression significantly decreased the expression of TNF-α and IFN-α induced by ZEN. Our findings revealed the effects of ZEN on the ISLR2 gene expression and indicated the ISLR2 gene as a novel regulator of ZEN-induced cytotoxicity, which provides potential molecular targets against ZEN toxicity.

Transcriptomic and epigenomic insights into pectoral muscle fiber formation at the late embryonic development in pure chicken lines

“…ISLR2 (immunoglobulin superfamily containing leucine-rich repeat 2) belongs to the leucine-rich repeat and immunoglobulin ( LIG ) family of membrane proteins ( Mandai et al, 2009 ). It regulates the immune response by reducing the expression of inflammatory factors ( Bi et al, 2022 ). Moreover, cis-Mendelian Randomization analysis has identified a causal relationship between ISLR2 and muscle wasting syndrome ( Bi et al, 2022 ).…”

“…It regulates the immune response by reducing the expression of inflammatory factors ( Bi et al, 2022 ). Moreover, cis-Mendelian Randomization analysis has identified a causal relationship between ISLR2 and muscle wasting syndrome ( Bi et al, 2022 ). Additionally, the LIG family protein encoded by ISLR2 is expressed in a subset of developing motor neurons and interacts with RTK to control axonal extension and branching ( Mandai et al, 2009 ).…”

Effect of atractylenolide III on zearalenone-induced Snail1-mediated epithelial–mesenchymal transition in porcine intestinal epithelium

“…IPEC-J2 cells were plated at a density of 3 × 10 4 cells/well in a 96-well plate. They were cultured for 24 h and subsequently treated with ZEA at concentrations of 0, 5, 10, 20, 40, and 80 μg/mL for 48 h [24]. The cells were then incubated for 2 h with water-soluble tetrazolium 1 (WST-1) (Roche Diagnostics GmbH, Mannheim, Germany).…”

Study of the ability of organic and mineral sorbents to sorption of secondary Fusarium metabolites

“…In low doses, T-2 toxin can have an immunotoxic effect on hematopoietic organs and cause leukopenia in the body. There is evidence of increased toxicity of the mycotoxins zearalenone and T-2 toxin when ingested together in animals [5,17,18]. One of the modern approaches to the problem of reducing the harmful effects of ecotoxicants is the use of various kinds of sorbents (clays, bentonites, zeolites, etc.…”