Analysis of the Salmonella typhimurium isolates from food-poisoning cases by molecular subtyping methods

Tsen, Hau‐Yang; Hu, Haihong; Lin, Jer-Sheng; Huang, Chieh-Chen; Wang, T.K

doi:10.1006/fmic.1999.0284

Cited by 31 publications

(26 citation statements)

References 28 publications

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“…The study showed, interestingly, that isolates of the same PT produced different PFGE patterns, and that the strains of different phage types had the same PFGE pattern For XbaI digested DNA, most of the strains belonged to the PFGE pattern X1 (10/15) and to the association X1/ PT4. Since the PFGE method has been shown to be highly discriminatory for subtyping of Salmonella spp including S. Enteritidis [41,42], strains with the same PFGE/phage type patterns, such as pattern X1/PT4, are likely to be closely related.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Ammari

Laglaoui²,

En-nanei

et al. 2009

J Infect Dev Ctries

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Objectives: This study aimed to investigate Salmonella Enteritidis strains isolated from human and food sources in the north of Morocco by means of phenotypic and genotypic methods. Methodology: Fifteen isolates from humans and food were submitted to phage typing, XbaI-macrorestriction (pulsed field gel electrophoresis [PFGE]), enterobacterial repetitive intergenic consensus (ERIC-PCR), antimicrobial susceptibility testing, and PCR assay targeting the spvR and invA genes. Results: Six fingerprinting profiles were obtained with the ERIC-PCR method, four with PFGE profiling, five with antimicrobial resistance, three with phage typing, and only one with plasmid profiling. spvR gene was detected in six strains, which did not harbour plasmids of 90 kb. Conclusions: The conclusions of this study are drawn from a limited number of isolates. It would be desirable to investigate a greater and more diverse population of Salmonella isolates. S. Enteritidis was genotyped and showed four different patterns by PFGE and six by ERIC-PCR. Accordingly, high genetic similarity and limited genetic diversity were found for these strains from north of Morocco.

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Section: Discussionmentioning

confidence: 99%

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Ammari

Laglaoui²,

En-nanei

et al. 2009

J Infect Dev Ctries

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“…In recent years, many DNA-based genotyping techniques have been utilized to assist in delineating epidemiological relationships between various isolates (Kostman, Edlind, LiPuma, & Stull 1992;Millemann, Lesage-Descauses, Lafont, & Chaslus-Dancla 1996;Tsen, Hu, Lin, Hung, & Wang 2000), being that PCR-ribotyping and PFGE have been applied in many studies because the former is considered not expensive and easy to perform, while the latter is currently the "gold standard" of the typing methods (Cerro, Soto, & Mendoza, 2003;Gatto et al, 2006;Oliveira et al, 2009;Soto, Guerra, Del Cerro, González-Hevia, & Mendoza, 2001;Swaminathan, Barret, Hunter, & Tauxe, 2001) .…”

Section: Introductionmentioning

confidence: 99%

Characterization of Salmonella Enteritidis isolated from human samples

Oliveira

Pasqualotto

Silva

et al. 2012

Food Research International

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“…Also, when 45 Salm. typhimurium strains were analysed, 26 PFGE patterns were obtained (Tsen et al . 2000).…”

Section: Introductionmentioning

confidence: 99%

Analysis of Salmonella enteritidis strains isolated from food-poisoning cases in Taiwan by pulsed field gel electrophoresis, plasmid profile and phage typing

Tsen¹,

Lin²

2001

J Appl Microbiol

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Aims: To establish the molecular typing data for Salmonella enteritidis due to its increasing role in Salmonella infections in Taiwan. Methods and Results: Sixty-three Salm. enteritidis strains isolated from related and unrelated patients suffering from food-borne poisoning during 1991±97 were collected and subjected to pulsed ®eld gel electrophoresis (PFGE), plasmid analysis and phage typing. For PFGE, XbaI, SpeI and NotI restriction enzymes were used for chromosomal DNA digestion. The results showed that, for these 63 Salmonella strains, 10 PFGE pattern combinations were found. Of these, pattern X3 S3 N3 was the major subtype, since 46 strains isolated from different locations at different times during 1991±97 showed this PFGE pattern. Plasmid analysis showed only three plasmid pro®les and phage typing showed that most of the Salmonella strains were of the phage type PT4. Conclusions: Most of the Salm. enteritidis strains circulating in Taiwan are of very similar genetic types or are highly related and that strains of PFGE pattern X3 S3 N3 are the prevalent and recirculating strains of Salm. enteritidis which caused food-poisoning cases in Taiwan in 1991±97. Signi®cance and Impact of the Study: This study provides information that in Salmonella infection, certain subtypes of Salm. enteritidis should be scrutinized.

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Analysis of the Salmonella typhimurium isolates from food-poisoning cases by molecular subtyping methods

Cited by 31 publications

References 28 publications

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Characterization of Salmonella Enteritidis isolated from foods and patients in northern Morocco

Characterization of Salmonella Enteritidis isolated from human samples

Analysis of Salmonella enteritidis strains isolated from food-poisoning cases in Taiwan by pulsed field gel electrophoresis, plasmid profile and phage typing

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