Analysis of the Seasonal Variations of the Concentration of air Functional Spores in the External Space of the School of Microbiology of the University of Costa Rica

Brizuela-Hernández, Marisol; Jaikel-Víquez, Daniela; Riggioni-Cordero, Olman; Escalante-Martínez, Ana Patricia; Gross, Norma T

doi:10.31080/asmi.2019.02.0369

Cited by 4 publications

(3 citation statements)

References 9 publications

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“…The ideal sampling period for these rooms was 15 min per location. The spores obtained, in situ, were counted and analyzed using a light microscope at 400X in order to examine their structure and to identify them 66 , 67 .…”

Section: Methodsmentioning

confidence: 99%

Multidisciplinary approach to the study of large-format oil paintings

Calderón-Mesén

Jaikel-Víquez

Barrantes-Madrigal

et al. 2023

Sci Rep

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Cultural heritage has become a keystone for comprehending our society, as it represents and reflects our origins, passions, beliefs and traditions. Furthermore, it provides fundamental information about specific temporary spaces, materials’ availability, technology, artist’s intention, and site weather conditions. Our aim was to develop a multidisciplinary approach with a main focus on investigating two Italian large-format paintings located in highly diverse environments such as the National Theater of Costa Rica. We monitored environmental conditions and quantified fungal aerial spores. Then, we determined regions of possible biodeterioration with the software MicroorganismPattern and used the software PigmentArrangement to elucidate the apparent colour of the paintings based on distribution and arrangement of the pigment crystals. Finally, we characterized eight genera of calcareous nannofossils found in the ground layers of the artwork. The former Men’s Canteen at the National Theater of Costa Rica presented a mean air temperature of 23.5 $$^{\circ }$$ ∘ C, a relative humidity of 72.7% and a concentration of CO$$_{2}$$ 2 of 570 ppm. The fungal aerial concentration was 1776 spores/m$$^{3}$$ 3 . The software MicroorganismPattern identified 32 sampling regions, out of which 11 were positive for microbial contamination. The software PigmentArrangement determined that the blue crystals (ultramarine pigment) had the shortest distances between themselves (29 $$\upmu$$ μ m). Finally, the nanofossils identified enabled us to restrict the age of the material to a biostratigraphic interval ranging from Coniacian to Maastricthian ages. By using a multidisciplinary approach we were able to explore the diptych, suggest a set of minimally invasive perspectives in tropical environments to be used worldwide and obtain key information about the artist’s artistic process, materials used along with better understand its state of conservation.

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Section: Methodsmentioning

confidence: 99%

Multidisciplinary approach to the study of large-format oil paintings

Calderón-Mesén

Jaikel-Víquez

Barrantes-Madrigal

et al. 2023

Sci Rep

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“…Cladosporium fungi is one of the largest, most heterogeneous genera of hyphomycetes, comprising more than 772 species, and including endophytic, fungicolous, human pathogenic, phytopathogenic, and saprobic species (Brizuela‐Hernandez et al, 2019). The aim of this study was to determine the potential of the Cladosporium species to biodegrade petroleum hydrocarbon and also to assess the effect of surfactant Tween 80 as an adjunct on the biodegradation process.…”

Section: Discussionmentioning

confidence: 99%

Assessing some Cladosporium species in the biodegradation of petroleum hydrocarbon for treating oil contamination

Bakri

2022

Journal of Applied Microbiology

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Aim: Biodegradation is a cost-effective and eco-friendly treatment for oilcontaminated materials using microorganisms. Bacteria and fungi can degrade petroleum by using it as an energy source and this may provide an enormous scope to remediate soils contaminated with petroleum and oil. This study aimed to assess the biodegradation of petroleum hydrocarbons by certain Cladosporium species.Methods and results: By using traditional and spectroscopic assessment analysis, qualitative screening was carried out using Cladosporium spores isolated from air and cultured on mineral salt medium supplemented with petroleum hydrocarbon as the sole carbon source, followed by quantitative assessment using gas chromatographymass spectroscopy. Nineteen Cladosporium strains from a total of 212 isolates exhibited remarkable capability to degrade petroleum hydrocarbon, representing four species (C. herbarum, C. macrocarpum, C. sphaerospermum, and C. cladosporioides).The results were expressed in terms of biodegradation percentage and optical density of hydrocarbon using a standard calibration curve. The highest reduction of petroleum hydrocarbon was observed with five Cladosporium strains belonging to two species (C. sphaerospermum and C. cladosporioides). Conclusion:This study succeeded in isolating several Cladosporium strains (from the air) with a high ability to degrade crude oil that can be used as biological agents to control petroleum pollution in soils and seas. The addition of a surfactant (Tween 80) enhanced the degradation of crude oil reaching a final concentration of 0.4%. Based on these results, the present study could indicate some unique prospects in the field of bioremediation and biodegradation of petroleum-contaminated soil.Significance and impact of study: This study gives unique prospects in the field of bioremediation and biodegradation of petroleum-contaminated soil.

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“…Es sustancial resaltar que los principales géneros fúngicos encontrados en el aire de centros educativos de las provincias de Heredia y San José (en nuestro país) fueron Cladosporium, Aspergillus y Penicillium (Jaikel-Víquez et al 2015;Brizuela-Hernández et al, 2019); situación que también ha sido reportada en otros países (Cooley et al, 1998). Estos géneros han sido asociados con reacciones alérgicas como el asma bronquial y el síndrome del edificio enfermo (Sick Building Syndrome) (Cooley et al, 1998;Joshi, 2008;Jaikel-Víquez y Riggioni-Cordero, 2012) y son agentes etiológicos de micosis superficiales como la onicomicosis (Tosti et al, 2000;Ramírez-Hobak et al, 2017) y sistémicas (Kousha et al, 2011;Chen et al, 2013;Sandoval-Denis et al, 2016).…”

Section: Microorganismosunclassified

La ciencia al servicio del arte: observación de dos obras pictóricas en acuarela de Fausto Pacheco por medio de análisis múltiple

Rivera-Romero

Jaikel-Víquez²,

Cob-Delgado³

et al. 2023

Herencia

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La conservación de las obras de arte es un aspecto fundamental de la inmensidad de la cultura humana y la expresión artística. Se analizaron dos obras en acuarela: Paisaje con casa campesina y El puente, pertenecientes a la colección del Banco Central de Costa Rica atribuidas al artista nacional Fausto Pacheco Hernández. Particularmente, el acercamiento exclusivo para estudiar dichas obras de arte está caracterizado por ser un análisis múltiple, donde se aplicaron técnicas no invasivas como la fotografía multiespectral. A partir de dicha técnica y el uso de herramientas computacionales novedosas como RegionsOfInterest y Golden Artists Colors se determinó información sobre la paleta pictórica y el proceso creativo del artista. El análisis múltiple permitió identificar diferentes patrones que podrían estar asociados con el crecimiento microbiano. Este estudio pionero corresponde al punto de partida para establecer indicadores que permitan, en el futuro, valorar la posible autenticidad de las pinturas con base en el establecimiento de la paleta pictórica del artista, los materiales utilizados, los procesos creativos y el contexto histórico. La aplicación de la ciencia para conocer las características de los materiales contribuye significativamente a determinar la manera más eficiente para conservar el patrimonio cultural.

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Analysis of the Seasonal Variations of the Concentration of air Functional Spores in the External Space of the School of Microbiology of the University of Costa Rica

Cited by 4 publications

References 9 publications

Multidisciplinary approach to the study of large-format oil paintings

Multidisciplinary approach to the study of large-format oil paintings

Assessing some Cladosporium species in the biodegradation of petroleum hydrocarbon for treating oil contamination

La ciencia al servicio del arte: observación de dos obras pictóricas en acuarela de Fausto Pacheco por medio de análisis múltiple

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