Analysis of the thaumatin-like genes of Rosa chinensis and functional analysis of the role of RcTLP6 in salt stress tolerance

Su, Lin; Zhao, Xiaojuan; Geng, Lifang; Fu, Lufeng; Lu, Yizeng; Liu, Qinghua; Jiang, Xinqiang

doi:10.1007/s00425-021-03778-y

Cited by 11 publications

(8 citation statements)

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“…The promoters of all PR5 family AsTLP genes contained cis -regulatory elements associated with the activation of immune mechanisms through responses to stresses (anaerobic conditions, dehydration, low temperature, salinization, heavy metals, and wounding), elicitors, and hormones, ( Figure 6 ). A similar set of elements has also been found in the promoters of Gossypium barbadense and Rosa chinensis TLP genes [ 73 , 74 ] and of the other garlic PR1–5 genes [ 50 , 60 ]. Accordingly, the expression of AsTLP genes may be triggered by phytohormones (ethylene, salicylic acid, ABA, and MeJA), and stresses.…”

Section: Discussionmentioning

confidence: 61%

Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection

Anisimova

Кочиева

Щенникова

et al. 2022

Plants

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Plant antifungal proteins include the pathogenesis-related (PR)-5 family of fungi- and other stress-responsive thaumatin-like proteins (TLPs). However, the information on the TLPs of garlic (Allium sativum L.), which is often infected with soil Fusarium fungi, is very limited. In the present study, we identified 32 TLP homologs in the A. sativum cv. Ershuizao genome, which may function in the defense against Fusarium attack. The promoters of A. sativumTLP (AsTLP) genes contained cis-acting elements associated with hormone signaling and response to various types of stress, including those caused by fungal pathogens and their elicitors. The expression of AsTLP genes in Fusarium-resistant and -susceptible garlic cultivars was differently regulated by F. proliferatum infection. Thus, in the roots the mRNA levels of AsTLP7–9 and 21 genes were increased in resistant and decreased in susceptible A. sativum cultivars, suggesting the involvement of these genes in the garlic response to F. proliferatum attack. Our results provide insights into the role of TLPs in garlic and may be useful for breeding programs to increase the resistance of Allium crops to Fusarium infections.

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Section: Discussionmentioning

confidence: 61%

Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection

Anisimova

Кочиева

Щенникова

et al. 2022

Plants

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“…RcMYB8 improved drought and salt tolerance by influencing ROS dynamic changes and proline accumulation. In addition, previous studies have shown a connection between RcMYB8 and RcPR5/1 (Su et al 2021 ), and RcPR5/1 was proved to enhance salinity resistance in rose (Su et al 2023 ). We next examined the relevant gene expression corresponding to ROS scavenging, proline synthase and pathogenesis-related-5 (PR5) in both RcMYB8 -silenced and RcMYB8 -overexpressing plants.…”

Section: Resultsmentioning

confidence: 92%

RcMYB8 enhances salt and drought tolerance in rose (Rosa chinensis) by modulating RcPR5/1 and RcP5CS1

Zhang,

Yu,

Niu

et al. 2024

Mol Horticulture

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Plant Myeloblastosis (MYB) proteins function crucially roles upon variegated abiotic stresses. Nonetheless, their effects and mechanisms in rose (Rosa chinensis) are not fully clarified. In this study, we characterized the effects of rose RcMYB8 under salt and drought tolerances. For induction of the RcMYB8 expression, NaCl and drought stress treatment were adopted. Rose plants overexpressing RcMYB8 displayed enhanced tolerance to salinity and drought stress, while silencing RcMYB8 resulted in decreased tolerance, as evidenced by lowered intra-leaf electrolyte leakage and callose deposition, as well as photosynthetic sustainment under stressed conditions. Here, we further show that RcMYB8 binds similarly to the promoters of RcPR5/1 and RcP5C51 in vivo and in vitro. Inhibiting RcP5CS1 by virus-induced gene silencing led to decreased drought tolerance through the reactive oxygen species (ROS) homeostatic regulation. RcP5CS1-silenced plants showed an increase in ion leakage and reduce of proline content, together with the content of malondialdehyde (MDA) increased, lowered activities of Catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD). Our study highlights the transcriptional modulator role of RcMYB8 in drought and salinity tolerances, which bridges RcPR5/1 and RcP5CS1 by promoting ROS scavenging. Besides, it is probably applicable to the rose plant engineering for enhancing their abiotic stress tolerances. Graphical Abstract

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“…The expression of TLPs can respond to a variety of stimuli, including salt, drought ( Su et al, 2021 ), SA, MeJA, ABA, and ethephon ( Singh et al, 2013 ), as well as host infections from a pathogenic virus ( Cornelissen et al, 1986 ) or fungus ( Wang et al, 2010 ). The fact that TLPs ’ expression was inducible by multiple signals suggests their respective transcription is under the control of a diverse set of cis -regulatory elements.…”

Section: Discussionmentioning

confidence: 99%

“…In Rosa chinensis , seven RcTLPs exhibited greater expression when exposed to drought or salinity stress. Three RcTLPs showed specific expression in leaves infected with Botrytis cinerea for 48 h ( Su et al, 2021 ). In wheat, 50 TaTLPs were differentially expressed when exposed to Blumeria graminis or Puccinia striiformis infection ( Sharma et al, 2022 ).…”

Section: Discussionmentioning

confidence: 99%

“…Thaumatin-like proteins are associated with plant defense responses against both biotic and abiotic stresses. The transcription of TLP genes can be induced significantly after exposure to drought ( Misra et al, 2016 ), freezing ( Kuwabara et al, 2002 ), and salinity ( Su et al, 2021 ) treatments, and transgenic lines overexpressing TLP genes could simultaneously exhibit resistance to biotic and abiotic stresses ( Chowdhury et al, 2017 ). When expressed in vitro , TLPs inhibited the growth of fungal pathogens by lysing spores, impeding spore germination, and hindering hyphal growth ( Abad et al, 1996 ; Rajam et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

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Genome-wide analysis of the Thaumatin-like gene family in Qingke (Hordeum vulgare L. var. nudum) uncovers candidates involved in plant defense against biotic and abiotic stresses

Wang

Yin

et al. 2022

Front. Plant Sci.

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Thaumatin-like proteins (TLPs) participate in the defense responses of plants as well as their growth and development processes, including seed germination. Yet the functioning of TLP family genes, in addition to key details of their encoded protein products, has not been thoroughly investigated for Qingke (Hordeum vulgare L. var. nudum). Here, a total of 36 TLP genes were identified in the genome of Qingke via HMM profiling. Of them, 25 TLPs contained a signal peptide at the N-terminus, with most proteins predicted to localize in the cytoplasm or outer membrane. Sequence alignment and motif analysis revealed that the five REDDD residues required for β-1,3-glucanase activity were conserved in 21 of the 36 Qingke TLPs. Phylogenetically, the TLPs in plants are clustered in 10 major groups. Our analysis of gene structure did not detect an intron in 15 Qingke TLPs whereas the other 21 did contain 1–7 introns. A diverse set of cis-acting motifs were found in the promoters of the 36 TLPs, including elements related to light, hormone, and stress responses, growth and development, circadian control, and binding sites of transcription factors, thus suggesting a multifaceted role of TLPs in Qingke. Expression analyses revealed the potential involvement of TLPs in plant defense against biotic and abiotic stresses. Taken together, the findings of this study deepen our understanding of the TLP family genes in Qingke, a staple food item in Tibet, which could strengthen future investigations of protein function in barley and its improved genetic engineering.

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Analysis of the thaumatin-like genes of Rosa chinensis and functional analysis of the role of RcTLP6 in salt stress tolerance

Cited by 11 publications

References 50 publications

Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection

Thaumatin-like Protein (TLP) Genes in Garlic (Allium sativum L.): Genome-Wide Identification, Characterization, and Expression in Response to Fusarium proliferatum Infection

RcMYB8 enhances salt and drought tolerance in rose (Rosa chinensis) by modulating RcPR5/1 and RcP5CS1

Genome-wide analysis of the Thaumatin-like gene family in Qingke (Hordeum vulgare L. var. nudum) uncovers candidates involved in plant defense against biotic and abiotic stresses

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